Optimization of media constituents for the production of lipase in solid state fermentation by Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)

Babu, I. Sarat; Kumari, Karanam Sita; Rao, Garapati Hanumantha

doi:10.4236/abb.2010.12016

Cited by 31 publications

(18 citation statements)

References 15 publications

(22 reference statements)

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“…Treichel et al [ 30 ] found that lipase production by C. rugosa in solid-state fermentation was optimal using substrates with 70% initial moisture. Similarly, other yeast strains and filamentous fungi also produced high lipase levels with 70% initial moisture [ 33 , 37 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

et al. 2016

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The aims of this work were to establish improved conditions for lipase production by Candida viswanathii using agroindustrial wastes in solid-state cultivation and to purify and evaluate the application of this enzyme for poultry fat hydrolysis. Mixed wheat bran plus spent barley grain (1 : 1, w/w) supplemented with 25.0% (w/w) olive oil increased the lipase production to 322.4%, compared to the initial conditions. When olive oil was replaced by poultry fat, the highest lipase production found at 40% (w/w) was 31.43 U/gds. By selecting, yeast extract supplementation (3.5%, w/w), cultivation temperature (30°C), and substrate moisture (40%, w/v), lipase production reached 157.33 U/gds. Lipase was purified by hydrophobic interaction chromatography, presenting a molecular weight of 18.5 kDa as determined by SDS-PAGE. The crude and purified enzyme showed optimum activity at pH 5.0 and 50°C and at pH 5.5 and 45°C, respectively. The estimated half-life at 50°C was of 23.5 h for crude lipase and 6.7 h at 40°C for purified lipase. Lipase presented high activity and stability in many organic solvents. Poultry fat hydrolysis was maximum at pH 4.0, reaching initial hydrolysis rate of 33.17 mmol/L/min. Thus, C. viswanathii lipase can be successfully produced by an economic and sustainable process and advantageously applied for poultry fat hydrolysis without an additional acidification step to recover the released fatty acids.

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Section: Discussionmentioning

confidence: 99%

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

et al. 2016

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“…The growing demand for palm oil has resulted in an increased supply of the waste product palm kernel cake (PKC). Of particular interest, this solid material contains 16.1% (w/w) protein and, when supplemented with glucose, was able to support the growth of Y. lipolytica ( Imandi et al, 2010 ). Under these conditions, lipase yields of 18.58 U/g of PKC were possible.…”

Section: Lipidsmentioning

confidence: 99%

Alternative Substrate Metabolism in Yarrowia lipolytica

et al. 2018

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Recent advances in genetic engineering capabilities have enabled the development of oleochemical producing strains of Yarrowia lipolytica. Much of the metabolic engineering effort has focused on pathway engineering of the product using glucose as the feedstock; however, alternative substrates, including various other hexose and pentose sugars, glycerol, lipids, acetate, and less-refined carbon feedstocks, have not received the same attention. In this review, we discuss recent work leading to better utilization of alternative substrates. This review aims to provide a comprehensive understanding of the current state of knowledge for alternative substrate utilization, suggest potential pathways identified through homology in the absence of prior characterization, discuss recent work that either identifies, endogenous or cryptic metabolism, and describe metabolic engineering to improve alternative substrate utilization. Finally, we describe the critical questions and challenges that remain for engineering Y. lipolytica for better alternative substrate utilization.

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“…Many substrates such as rice bran [80], coconut oil cake [65], olive cake and sugar cane bagasse [81], gingelly oil cake [67], babassu oil cake [74], wheat bran [82], Jatropha curcas seed cake [83], niger seed oil cake [84], palm kernal cake [84], groundnut oil cake [85] and mustard oil cake [86] have been used successfully for lipase production. The most important process parameters that have been investigated and optimized up to now are the incubation time, inoculum age, inoculum size, initial moisture content, nature and concentration of carbon and nitrogen sources as well as the C/N ratio of the medium [87].…”

Section: Solid Media Solid Support and Ambient Factors Used For Lipamentioning

confidence: 99%

Fungal Lipase Production by Solid-State Fermentation

Ramos-Sánchez¹,

Cujilema-Quitio²,

Julián-Ricardo³

et al. 2015

J Bioprocess Biotech

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Lipases are one of the most promising enzymes in the chemical and biopharmaceutical industries. Numerous applications have been reported including fine chemistry, detergents formulation and biodiesel synthesis. Lipases are commonly produced by a wide variety of yeasts and filamentous fungi in submerged fermentation or solid-state fermentation. Filamentous fungi and yeasts usually behave more efficiently in solid-state fermentation and show greater productivities when compared to submerged fermentation. Although filamentous fungi adequately growth in solid-state fermentation, there are some limitations for cultivating them by this process. Here, a review is made about lipase production in solid-state fermentation. This includes the analysis of solid-state fermentation as a promising technology, characterization of growth media and ambient factors such as moisture, pH and temperature.

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Optimization of media constituents for the production of lipase in solid state fermentation by Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)

Cited by 31 publications

References 15 publications

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

Alternative Substrate Metabolism in Yarrowia lipolytica

Fungal Lipase Production by Solid-State Fermentation

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