Optimization of <i>Boesenbergia rotunda</i> Extract-Loaded Polyvinyl Alcohol Hydrogel Wound Dressing by Box-Behnken Design

Abstract

The objective of this study was to optimize fabrication variables that affected desirable properties of dressings. Boesenbergia rotunda extract incorporated PVA hydrogels for wound dressings were fabricated by freeze-thaw method. The fabrication variables including PVA concentration (15, 17.5 and 20 % w/w), freeze-thaw cycle (2, 3 and 4 cycles) and extract loading (30, 40 and 50 % w/w) were studied and optimized. Effects of variables on the hydrogel wound dressing properties were determined by using Box-Behnken design and response surface method. Hydrogel properties such as tensile strength, elongation at break, Young’s Modulus, water content, swelling and erosion were measured and used as the designed responses. From statistical data analysis (p &lt;0.05), the polynomial quadratic equation which indicated the significant effects of fabrication variables on the hydrogel properties was generated. In conclusion, desirable B. rotunda extract loaded PVA hydrogel dressing was favorably designed. The optimized PVA concentration, freeze-thaw cycle and extract loading were 17.07 % w/w, 3.86 cycles and 50 % w/w, respectively.

Design of PVA/Chitosan Loaded with Chromolaena Odorata Extract as Wound Dressings

“…For erosion, it could be seen that the similar trend of increasing erosion rate as the PVA concentration increases. As being reported by Eakwaropas [19], erosion rate of the fabricated hydrogel were corresponding with the increased of PVA concentration as well as higher extract loading in the formulations.…”

“…Erosion percentage represents the crosslinking strength of hydrogel [19]. Samples with the size of 10 mm x 10 mm were weighed and immersed in PBS, pH 7.4.…”

“…The samples of size 10 mm x10mm were cut and weighed and its initial weight were recorded according to the method described by Eakwaropas [19]. Samples were immersed in simulated wound fluid (SWF) pH 7.4 and incubated at 37°C.…”

Antioxidant Activity, Antimicrobial Activities, and Effect on the Viability of Fibroblast Cells of <i>Boesenbergia rotunda</i>- Loaded Hydrogel Patches

“…B. rotunda extract-loaded hydrogel patches were prepared by a freeze-thaw method following the method described previously. The optimal hydrogel patches contained PVA and B. rotunda extract solution at the concentrations of 17.07 and 50 % w/w, respectively [9]. Antioxidant activity of hydrogel patches.…”