Optimization of liquid culture conditions of Philippine wild edible mushrooms as potential source of bioactive lipids

Dulay, Rich Milton R.; Ray, Karen; Hou, Ching T.

doi:10.1016/j.bcab.2015.04.003

Cited by 25 publications

(24 citation statements)

References 24 publications

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“…The present study suggests that the three V. volvacea strains have greater preference for the combination of peptone and dextrose in the medium. This finding conforms with the study of Dulay et al [9] who reported that Sabouraud dextrose broth produced the highest biomass yield of Schizophyllum commune, Ganoderma lucidum, V. volvacea, and Pleurotus cystidiosus in submerged culture. However, in the evaluation of mycelial growth using indigenous culture media, the two strains of V. volvacea (Rang-ayan and CLSU) showed the maximum mycelial growth on potato sucrose gulaman (PSG) and corn grit gulaman (CGG) [8].…”

Section: Effect Of Culture Media and Phsupporting

confidence: 93%

“…Similarly, Rang-ayan and CLSU strains of V. volvacea showed favorable mycelial growth at a wide range of pH with very thick mycelia at pH 7.5-8.0 [8]. In liquid culture, the optimum pH of V. volvacea was at pH 6.0 [9]. However, other species of Volvariella such as V. esculenta could produce good quality mycelia at pH 6 [11], and V. speciosa mycelia could grow at pH 3-9 [12].…”

Section: Effect Of Culture Media and Phmentioning

confidence: 92%

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Effects of culture media and physical factors on the mycelial growth of the three wild strains of Volvariella volvacea from Ecuador

Abon¹,

Dulay²,

Kalaw³

et al. 2020

J App Biol Biotech.

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Volvariella volvacea is an edible and nutraceutical tropical mushroom species. This study reports the optimal culture conditions for efficient growth of mycelia of the three wild strains of V. volvacea (La Clementina, Vinces, and Montalvo) from Ecuador. The mycelial growths were evaluated on the three culture media, pH, aeration, illumination, and temperature. Mycelia of the three strains grew best on Sabouraud dextrose agar at pH 6.5-7.5 when incubated in either sealed and unsealed conditions at 26-28°C and 32°C. Vinces and Montalvo strains favored dark and lighted conditions, respectively, whereas La Clementina favored both. Therefore, the three exotic strains of V. volvacea could be commercially cultivated in the Philippines and utilized in various applications. Fruiting body of V. volvacea has grayish-to-black egg-shaped volva at young and rupture to expand the pileus up to nearly flat. Nutritionally, the fruiting bodies contain carbohydrates, sugar, protein, crude fiber, ash, fats, vitamins, organic acids, and bioactive metabolites that contribute immensely to umami taste and aroma and notable biological

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Section: Effect Of Culture Media and Phsupporting

confidence: 93%

Section: Effect Of Culture Media and Phmentioning

confidence: 92%

Effects of culture media and physical factors on the mycelial growth of the three wild strains of Volvariella volvacea from Ecuador

Abon¹,

Dulay²,

Kalaw³

et al. 2020

J App Biol Biotech.

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“…Outra classe de compostos bastante presente em corpos de frutificação de basidiomicetos são os lipídeos. Segundo Dulay 33 , aproximadamente 5,7% do peso seco dos cogumelos correspondem às substâncias oleosas, dentre elas os óleos, terpenoides, esteroides e ácidos graxos, com grande potencial farmacológico.…”

Section: Resultsunclassified

Chemical Composition and Biological Potential of Mycelium Extract from Basidiomicete Tyromyces pulcherrimus (Rodway) G. Cunn. Cultivated in Liquid Medium

et al. 2020

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The exploration of microorganisms as a source of biologically active substances have been performed since the discovery of the first fungal metabolite of great medical importance, the penicillin. Thus, the main objective of this study was to evaluate the chemical components in the extract of mycelium of Tyromyces pulcherrimus, and to evaluate the potential in vitro antibacterial, antioxidant and inhibitory activities of alpha-glucosidase and acetylcholinesterase enzymes. The classes of secondary metabolites detected by thin layer chromatography (TLC) were flavonoids, phenols and lipids. An analysis by high-performance liquid chromatography coupled with mass spectrometry (HPLC-ESI-MS/ MS) comparing to the 47 standards of phenolic compounds, led to the identification of ellagic acid (4.55 ± 0.15 μg g-1). The extract showed weak antibacterial activity for Pseudomonas aeruginosa (ATCC 27853) and showed no antibacterial activity against Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923). In anticholinesterase activity, 99 ± 0.1% of the enzyme was inhibited at a concentration of 1 mg mL-1. At the same concentration, the sample showed weak antioxidant activity on the DPPH assay (18.18 ± 0.04%), and weak inhibitory activity of the enzyme alphaglycosidase IC50 of 15.19 ± 5.52%. This study suggests that the anticholinesterase activity in this species is promising and may be involved with the compounds detected by TLC and mass spectrometry. A exploração dos microorganismos como fonte de substâncias biologicamente ativas tem sido realizada desde a descoberta do primeiro metabólito fúngico de importância na medicina, a penicilina. O objetivo principal deste estudo foi avaliar os componentes químicos presentes no extrato do micélio fúngico da espécie Tyromyces pulcherrimus, e avaliar as potenciais atividades antibacteriana, antioxidante e inibitória das enzimas alfaglicosidase e acetilcolinesterase in vitro presentes na espécie. As classes de metabólitos secundários detectados por cromatografia em camada delgada (CCD) foram flavonoides, fenóis e lipídios. Uma análise por cromatografia líquida de alta eficiência acoplada a espectrometria de massas (HPLC-ESI-MS/MS), em comparação a 47 padrões de compostos fenólicos, permitiu a detecção do ácido elágico (4,55 ± 0,15 μg g-1). O extrato mostrou fraca atividade antibacteriana para Pseudomonas aeruginosa (ATCC 27853) e não mostrou atividade antibacteriana frente à Escherichia coli (ATCC 25922) e Staphylococcus aureus (ATCC 25923). Na atividade anticolinesterásica, houve inibição de 99 ± 0,1% da enzima na concentração de 1 mg mL-1. Nesta mesma concentração, a amostra apresentou fraca atividade antioxidante no ensaio com o radical livre DPPH (18,18 ± 0,04%) e fraca atividade inibitória da enzima alfa-glicosidase (15,19 ± 5,52%). Este estudo sugere que a atividade anticolinesterásica nesta espécie é promissora e pode estar envolvida com os compostos detectados por CCD e espectrometria de massas.

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“…The precipitated biomass was washed three times with distilled water and freeze-dried to give the biomass dry mass. Finally, the biomass dry mass of mycelium was measured [9][10].…”

Section: Determination Of the Biomass In Fermentation Liquidmentioning

confidence: 99%

Using A New Mushroom Shake Flask Culture Device to Develop Suillellus Luridus

Hu¹,

Duan²,

You³

et al. 2017

Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

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Abstract-A new shake flask culture device was introduced in this paper, whose main part was comprised with mouth, body and bottom. Besides, it also included cork; pump; small bacteria filter and etc. So, the utility model had the advantages of simple structure, artful design, and it was more convenient to use. Compared with the ordinary shake flask, it solved the problem of using shaking table, occupying too much space, and avoided the low yield of mycelium. Furthermore, the culture success rate was significantly higher than that of the shaking table culture. This device can also prevent the sealing gauze from being easily polluted by medium in the fermentation process, ensuring good ventilation from fermentation flask and avoiding pollution at the same time. Suillellus luridus were cultured by the two kinds of fermentation devices respectively, and the highest mycelia biomass from the new shake flask culture device could achieve 2.32 g/L, while that of the normal fermentation device only could achieve 2.11 g/L.

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Optimization of liquid culture conditions of Philippine wild edible mushrooms as potential source of bioactive lipids

Cited by 25 publications

References 24 publications

Effects of culture media and physical factors on the mycelial growth of the three wild strains of Volvariella volvacea from Ecuador

Effects of culture media and physical factors on the mycelial growth of the three wild strains of Volvariella volvacea from Ecuador

Chemical Composition and Biological Potential of Mycelium Extract from Basidiomicete Tyromyces pulcherrimus (Rodway) G. Cunn. Cultivated in Liquid Medium

Using A New Mushroom Shake Flask Culture Device to Develop Suillellus Luridus

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