Optimization of lipospheres production by factorial design and their performances on a dielectrophoretic lab-on-a-chip platform

Tosi, Azzurra; Mazzitelli, Stefania; Capretto, Lorenzo; Guerrieri, R.; Nastruzzi, Claudio

doi:10.1016/j.colsurfa.2009.03.005

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…CA microspheres (CAM) and Eudragit RS microspheres (ERSM), lipospheres (LS) and corn oil based-emulsion (COBE) were prepared following the experimental procedures described in the ESI †. CAM were prepared by solvent evaporation technique, 21 ERSM by an in-liquid drying process from an oil-in-oil system, 22 LS by melt dispersion technique 23 and COBE by a microfluidic flow focusing technique. 24 Drug release experiments were performed as previously described.…”

Section: Production Of Drug Delivery Systems (Dds)mentioning

confidence: 99%

“…The DDS included into the alginate microfibres were produced and characterized in our laboratory, namely: CAM, 21 ERSM, 22 (Fig. 3A-C), LS 23 and COBE. 24 In addition, different cell types were also embedded into the microfibres, they include the human myeloid leukemia K562 (representing a stable cell line growing in suspension) and the Wharton's Jelly Mesenchymal Stem Cells (WJMSC) (representing a primary cell population isolated from the umbilical cord) (Fig.…”

Section: Production Of Multifunctional Alginate Microfibres By Single...mentioning

confidence: 99%

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Optimised production of multifunctional microfibres by microfluidic chip technology for tissue engineering applications

et al. 2011

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This paper describes a method for the production of alginate microfibres using glass-based microfluidic chips fabricated by a photolithography-wet etching procedure. The main focus of the work is the fabrication of a cell containing multifunctional microfibres which have great potential for applications in drug release formulations and tissue engineering scaffolds (to guide the regeneration of tissues in predefined sizes and shapes) providing cell structural support and immunoisolation. The key parameters, which critically influence the formation of microfibres and their geometries, were identified by a classical intuitive approach COST (Changing One Separate factor a Time). In particular, their effects on the microfibre diameter were investigated, which are directly associated with their functionalities relating to the implantation site, the nutrient availability and diffusion/transport of oxygen, essential nutrients, growth factors, metabolic waste and secretory products. The interplay between the alginate solution concentration, pumping rate and gelling bath concentration in controlling the diameter of the produced microfibres was investigated with a statistical approach by means of a "design of the experiments" (DoEs) optimization and screening. Finally, the processing impacts on cell viability, the cellular effect of wall thickness consistency and the spatial distribution of cells within the alginate microfibre were examined. We provide an approach for the production of alginate microfibres with controlled shape and content, which could be further developed for scaling up and working towards FDA approval.

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Section: Production Of Drug Delivery Systems (Dds)mentioning

confidence: 99%

Section: Production Of Multifunctional Alginate Microfibres By Single...mentioning

confidence: 99%

Optimised production of multifunctional microfibres by microfluidic chip technology for tissue engineering applications

et al. 2011

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“…[ 19 ] Cytotoxic effects after phagocytosis, toxic effects of organic residues after the production of polymers and lack of industrial-scale up made their practice restricted. [ 67 ]…”

Section: Limitationsmentioning

confidence: 99%

A perspective overview on lipospheres as lipid carrier systems

Dudala¹,

Yalavarthi²,

Vadlamudi³

et al. 2014

Int J Pharma Investig

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Both hydrophilic and lipophilic therapeutics can be delivered successfully into deep and peripheral tissues such as cerebrospinal fluid and central nervous system by encapsulating them with crystalline lipids as lipospheres. The advent of lipospheres was meant to deliver both therapeutic moieties with enhanced efficacy and added stability to reach out intended tissue areas. Although extensive information is available on physicochemical, analytical and biopharmaceutical aspects of lipospheres, there was no specific order pertaining to critical composition and rationale of component selection available for academic and pilot scale processing of lipospheres. With the interest of compiling key points in a typical formulation of lipid lipospheres, this article was intrigued to discuss melt method, co-solvent, microemulsion, super critical fluid, spray drying and spray congealing techniques that were employed to scale up lipospheres. The selection criteria for both the drugs and lipids in liposphere formulations were demonstrated here. The quality assessment with variables like loading capacity and entrapment efficiency was explained. A note on preliminary screening factors to determine the liposphere formation such as liposphere dimensions with morphological scenario was detailed in this article. This article also includes the stability and storage issues with reference to photolysis. The marked differential in enhancing solubility and permeability characteristics of Class II and IV drug candidates by liposphere delivery systems with an evident of in vivo outcomes were emphasized.

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“…Many lipids form micelles, others organize themself as lipid bilayers with the hydrophobic tails lined up against one another and the hydrophilic head-group facing the water on both sides. These unique features make lipids most suitable to be used as excipients for advanced drug delivery formulations, since it is increasingly apparent that formulations based on natural and/or synthetic lipids represent a viable means for enhancing the drug bioavailability of poorly water-soluble, highly lipophilic drugs [12].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Drug Release Profiles from Formulations Based on Micro and Nano Systems

Esposito

Mazzitelli

Cortesi

et al. 2012

CAC

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The development of drug delivery systems in experimental therapy usually requires in vitro release models, that should posses specific characteristics including: low cost, simple procedure, high reproducibility and very importantly resemble as strictly as possible the in vivo behaviour. In this respect, the paper describes the effects of the use of different experimental procedures on the drug release profiles from controlled delivery formulations based on nano and micro systems. As examples of micro and nanosystems, microparticles constituted of poly-lactide-co-glycolide (PEM) or gelatine (GEM) and nanostructured lipid carriers (NLC) or cubosomes (CBS) were selected, respectively. All the analysed formulations contained bromocriptine (BC) that represents a poorly water-soluble drug. The influence of the experimental release method and of release media has been investigated using different experimental setup including direct and reverse dialysis, flow-through cell, USP XXII paddle and Franz cell methods.

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Optimization of lipospheres production by factorial design and their performances on a dielectrophoretic lab-on-a-chip platform

Cited by 7 publications

References 21 publications

Optimised production of multifunctional microfibres by microfluidic chip technology for tissue engineering applications

Optimised production of multifunctional microfibres by microfluidic chip technology for tissue engineering applications

A perspective overview on lipospheres as lipid carrier systems

Analysis of the Drug Release Profiles from Formulations Based on Micro and Nano Systems

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