Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions

Oliveira, Felisbela Maria Araújo; Souza, Carlos Eduardo Conceição de; Peclat, Veronica Regina Oliveira Lopes; Salgado, José Manuel; Ribeiro, Bernardo Dias; Coelho, Maria Alice Zarur; Venâncio, Armando; Belo, Isabel

doi:10.1016/j.fbp.2017.01.007

Cited by 64 publications

(29 citation statements)

References 51 publications

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“…The use of olive pomace as the sole substrate in SSF is difficult; thus, wheat bran is frequently used as a supplement to improve enzyme production. [36][37][38] However, the cost of wheat bran is high relative to other agro-industrial wastes, such as winery wastes.…”

Section: Effect Of the Substrates On Enzyme Production By Ssfmentioning

confidence: 99%

Improved lignocellulolytic enzyme production and antioxidant extraction using solid‐state fermentation of olive pomace mixed with winery waste

Filipe¹,

Fernandes²,

Castro³

et al. 2019

Biofuels Bioprod Bioref

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Olive pomace is characterized by its low nutritional value and high phenolic content, which hinders its direct use as animal feed, fertilizer, or as a substrate in bioprocesses such as solid-state fermentation (SSF). A possible strategy for bioprocessing olive pomace by SSF is the mixture of olive mill wastes with other wastes produced in the same region, such as winery wastes. This may improve the production of bioactive compounds like enzymes and antioxidant phenolics. A simplex-centroid design was used to evaluate the use of olive mill and winery wastes alone or in combination as a substrate for SSF with Aspergillus niger and Aspergillus ibericus. Synergistic effects of combinations of crude olive pomace (COP), exhausted olive pomace (EOP), vine trimming shoots (VTS), and exhausted grape marc (EGM) were observed in the production of xylanases, cellulases, β-glucosidases, and in the variation in total phenolics and antioxidant activity of SFF extracts. A multiple response optimization was carried out, leading to the following optimal mixture of substrates: for A. niger, 23% (w/w) COP, 30% EGM, 33% VTS, 14% EOP; for A. ibericus, 30% EGM, 36% VTS, 34% EOP. The scale-up to tray bioreactor with optimal substrate made it possible to achieve the maximum xylanase, cellulase, and β-glucosidase production of 189.1 ± 26.7, 56.3 ± 2.1 and 10.9 ± 0.8 U/g, respectively. The antioxidant activity of fermented wastes was also improved 2.2-fold as compared with unfermented wastes. Thus, a combination of olive mill and winery wastes in SSF is a potential strategy to increase their value and to develop a circular strategy in these industries.

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Section: Effect Of the Substrates On Enzyme Production By Ssfmentioning

confidence: 99%

Improved lignocellulolytic enzyme production and antioxidant extraction using solid‐state fermentation of olive pomace mixed with winery waste

Filipe¹,

Fernandes²,

Castro³

et al. 2019

Biofuels Bioprod Bioref

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“…These substrates have low commercial value and even impose attention and care regarding their environmental impact. Because growth substrates constitute a major share (approximately 40%) in the production costs, usage of low‐cost substrates clearly can foremost contribute to a lower enzyme price .…”

Section: Introductionmentioning

confidence: 99%

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Salim

Grbavčić

Šekuljica

et al. 2019

Biotech and App Biochem

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Protease, cellulase, and α-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and α-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity (ࣙ7.5 IU).

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“…Lipases are ubiquitous in nature and are produced by plants, animals and microorganisms [4,10]. However, microbial lipases form the most widely used class of enzymes due to their selectivity, lower production cost, ease of genetic manipulation, high productivity, regiospecificity, stereospecificity, broad substrate specificity and ability to catalyze heterogeneous reactions at the interface of water soluble and water insoluble systems [8,[11][12][13]. Microorganisms such as bacteria, yeast, fungi and actinomycetes have been reported as potential producers of lipase [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

Adetunji

Olaniran

2018

Biotechnology & Biotechnological Equipment

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Lipases are enzymes that hydrolyze fats into fatty acids and glycerol at the water-lipid interface and are also involved in a variety of bioconversion reactions in non-aqueous and micro-aqueous environments. In this study, we optimized the culture conditions for extracellular lipase production by an indigenous lipase-producing bacterial strain isolated from lipid-rich wastewater, using response surface methodology. The studied isolate was identified as Bacillus aryabhattai SE3-PB by polymerase chain reaction and analysis of 16S rDNA. Sunflower oil was found to induce maximum lipase production. Face centered central composite design revealed that temperature (40 C), pH (7.6), inoculum volume (2.8%, v/v), agitation (193 rpm) and inducer oil concentration (2%, v/v) significantly influenced lipase production at the respective optimum conditions. The coincidence of observed lipase production (264.02 ± 1.94 U/mL) with predicted lipase yield (265.82 U/mL) coupled with a high correlation coefficient (R 2 ¼ 0.9919, P < 0.01) confirmed the validity of the model. A 7.2-fold increase in lipase production was obtained in the optimized medium compared to the basal medium. These findings provide the first report on lipase production and optimization by B. aryabhattai SE3-PB and suggest a rational choice of optimum processing conditions for commercial lipase production by B. aryabhattai SE3-PB.

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Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions

Cited by 64 publications

References 51 publications

Improved lignocellulolytic enzyme production and antioxidant extraction using solid‐state fermentation of olive pomace mixed with winery waste

Improved lignocellulolytic enzyme production and antioxidant extraction using solid‐state fermentation of olive pomace mixed with winery waste

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

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