Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

Abstract

Lipases are enzymes that hydrolyze fats into fatty acids and glycerol at the water-lipid interface and are also involved in a variety of bioconversion reactions in non-aqueous and micro-aqueous environments. In this study, we optimized the culture conditions for extracellular lipase production by an indigenous lipase-producing bacterial strain isolated from lipid-rich wastewater, using response surface methodology. The studied isolate was identified as Bacillus aryabhattai SE3-PB by polymerase chain reaction and analysis of 16S rDNA. Sunflower oil was found to induce maximum lipase production. Face centered central composite design revealed that temperature (40 C), pH (7.6), inoculum volume (2.8%, v/v), agitation (193 rpm) and inducer oil concentration (2%, v/v) significantly influenced lipase production at the respective optimum conditions. The coincidence of observed lipase production (264.02 ± 1.94 U/mL) with predicted lipase yield (265.82 U/mL) coupled with a high correlation coefficient (R 2 ¼ 0.9919, P < 0.01) confirmed the validity of the model. A 7.2-fold increase in lipase production was obtained in the optimized medium compared to the basal medium. These findings provide the first report on lipase production and optimization by B. aryabhattai SE3-PB and suggest a rational choice of optimum processing conditions for commercial lipase production by B. aryabhattai SE3-PB.

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

“…The F-value of 58.97 indicates that the model is significant and the Lack of Fit (LOF) F-value of 2.56 is insignificant. A coefficient of determination (R 2 ) closer to 1 indicates a better correlation between the predicted and observed values, and a smaller coefficient of variation (C.V.%) implies a higher reliability of the experiment [35,44]. In this study, the R 2 of 0.9823, predicted R of 0.9538 and adjusted R of 0.9826 were consistent, and the predicted R and adjusted R were within 0.20 of each other.…”

Optimization of Lactobacillus Plantarum Activities in the Biosinthesis of Lipase Enzymes

“…Corn oil has the same fat content as olive oil and palm oil, although the amount is different [17]. Palm oil and olive have used to induce maximum lipase production by Bacillus aryabhattai SE3-PB [18].…”

Thermostable acidic lipase of Bacillus glycinifermentans-MK840989 isolated from contaminated environment; its optimization, purification and exploring potential applications

“…B. glycinifermentans-MK840989 cells were induced by olive oil (Figure 2) which agrees with Liaqat et al 19 but contradicts with the findings of Iqbal and Rehman, 9 who observed slow growth of B. subtilis I-4 cells in the presence of olive oil. Adentunji and Olaniran, 30…”