Optimization of lipase production by Candida cylindracea in palm oil mill effluent based medium using statistical experimental design

Salihu, Aliyu; Alam, Md. Zahangir; AbdulKarim, M. Ismail; Salleh, Hamzah Mohd.

doi:10.1016/j.molcatb.2010.12.012

Cited by 114 publications

(84 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2b). The same results were observed by Salihu et al (2011), who reported a significant reduction in the lipolytic activity of Candida cylindracea after the insertion of NH 4 Cl to the culture medium. In the study by Bora and Bora (2012), it was reported that various inorganic nitrogen sources including ammonium sulphate and urea enhanced lipase production of Bacillus sp.…”

Section: Resultssupporting

confidence: 85%

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Ameri¹,

Shakibaie²,

Amirpour-R³

et al. 2015

Biotechnology

View full text Add to dashboard Cite

A B S T R A C T A thermoalkalophilic lipase producing bacterial strain, identified asBacillus atrophaeus FSHM2 using 16S rDNA sequencing analysis was isolated from salty soil and its lipase was partially purified and characterized. The obtained results revealed that glucose, hazelnut oil, urea and calcium ion positively affected the lipase production by increasing the lipolytic activity to 13582.5, 6270, 4442 and 5505 U LG 1 , respectively compared to that of basal medium (4150 U LG 1 ). The partially purified lipase acted optimally at pH 9 and retained 88.2% of its initial activity after 1 h of incubation at 100°C. A two fold increase in the relative activity of the partially purified lipase was obtained in the presence of 4 M of NaCl. Application of the partially purified lipase for the synthesis of ethyl and methyl valerate in the organic solvent medium (xylene) resulted in 81.6 and 62.4% esterification, respectively, after 24 h of incubation.

show abstract

Section: Resultssupporting

confidence: 85%

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Ameri¹,

Shakibaie²,

Amirpour-R³

et al. 2015

Biotechnology

View full text Add to dashboard Cite

show abstract

“…It was also observed that beyond this optimum temperature, the water content in the medium was reduced which badly affected the synthesis of enzyme and microbial growth. The enzyme also gets denatured as also observed by other workers [31,32].…”

Section: Effect Of Temperaturesupporting

confidence: 77%

Production of an extracellular lipase by Candida utilis NRRL-Y-900 using agro-industrial by-products

Rehman¹,

Rasool²,

Mukhtar³

et al. 2014

Turk J Bioch

View full text Add to dashboard Cite

“…Candida cylindracea cultured in palm oil mill effluent (Salihu et al 2011) and olive mill effluent (Brozzoli et al 2009;D'Annibale et al 2006) showed appreciable levels of lipase production. Similarly, grease waste has been used as a substrate for lipase production by P. chrysogenum under solid-state fermentation (Kumar et al 2011).…”

Section: Molecular Studiesmentioning

confidence: 99%

“…Rhizopus delemar (Acıkel et al 2010), A. carneus (Kaushik et al 2006), Aspergillus awamori (Basheer et al 2011), Geotrichum sp. (Burkert et al 2004), C. cylindracea (Salihu et al 2011), Burkholderia sp. (Gupta et al 2007), Stenotrophomonas sp.…”

Section: The Use Of Statistical Tools For Optimizing Lipase Productionmentioning

confidence: 99%

An insight into microbial lipases and their environmental facet

Kanmani¹,

Aravind²,

Kumaresan³

2014

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Lipases are serine hydrolases that catalyze the hydrolysis and synthesis of esters formed from glycerol and long-chain fatty acids, by acting at the oil-water interface. Lipases from microbial sources have received heightened attention for an array of industrial applications, and these enzymes have been well exploited in the environmental sector as well. In this article, we present an overview of microbial lipase, including the microorganisms from which it could be produced; the application of recombinant DNA technology tools to produce lipase with enhanced properties, the effective use of waste materials as substrates for lipase production; the usage of statistical tools to efficiently optimize the production medium; lipase purification strategies; and the immobilization of the enzyme on a variety of support materials. The next section of the article provides a gist of its application in diversified spheres and focusses exclusively on the environmentally relevant ones. Lipasecatalyzed esterification, transesterification, and interesterification reactions, an emerging area of green chemistry; lipase-mediated in vitro biopolymer synthesis and degradation; and the application of lipase for remediating fat and oil constituents in wastewater are dealt with in-depth. When its full potential is harnessed, the enzyme could play a pivotal role in environmental management.

show abstract

Optimization of lipase production by Candida cylindracea in palm oil mill effluent based medium using statistical experimental design

Cited by 114 publications

References 31 publications

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Production of an extracellular lipase by Candida utilis NRRL-Y-900 using agro-industrial by-products

An insight into microbial lipases and their environmental facet

Contact Info

Product

Resources

About