Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Ameri, Atefeh; Shakibaie, Mojtaba; Amirpour-R, Sahar; Ameri, Alieh; Adeli-Sard, Mahboubeh; Khazaeli, Payam; Rahmani, Hamid Reza; Forootanfa, Hamid

doi:10.3923/biotech.2015.154.164

Cited by 6 publications

(2 citation statements)

References 31 publications

(67 reference statements)

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“…The colonies of lipase producing bacteria were showed halos of orange fluorescent during 48hrs of incubation at 37°C, This appearance may be due to the hydrolysis of substrate, or due to the construction of conjugate of a Rhodamine -B long chain fatty acid. The results in the present study agree with study of 21,13,[22][23][24][25][26] . Qualitative screening of Pseudomonas sp LSK25 lipase showed positive evidence of lipase production.…”

Section: Investigation Screening Of Lipase Producing Bacteriasupporting

confidence: 93%

Determination the Lipase Activity of Staphylococcus sp. Strain Isolated from Clinical Specimens

Alkabee¹,

Al-Salami²,

Ansari³

2020

J. Pure Appl. Microbiol.

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Bacterial lipases are the most important collection of biocatalysts used for a variety of biotechnological applications. In the current study the morphological, biochemical and Molecular characteristics to the extracellular lipase producing bacteria were identified. The bacterial isolates were selected as lipase producing bacteria using Rhodamine-B agar plate. the production of Lipase from the organism were determined with varying incubation temperature (20 to 50°C) and range of incubation time (16-120) hrs. 16 bacterial isolates were identified as Staphylococcus hemolyticus strain SHKS1 with Genbank acc. MK977614.1. By comparing the observed DNA sequences of these local samples with the retrieved DNA sequences (GenBank acc. MG595372.1) as showed high lipase activity according to experimental conditions about 140 U/ml. The current study showed that enzymatic activity of crude lipase extract was maximum in 72 hrs incubation period and 37°C.

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Section: Investigation Screening Of Lipase Producing Bacteriasupporting

confidence: 93%

Determination the Lipase Activity of Staphylococcus sp. Strain Isolated from Clinical Specimens

Alkabee¹,

Al-Salami²,

Ansari³

2020

J. Pure Appl. Microbiol.

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“…The operation of biotechnological techniques at high thermal levels offers several benefits, and one example is temperature increase has a considerable effect on the solubility and bioavailability of organic compounds (Ameri et al, 2015). Consequently, several thermo-active amylases have been isolated and characterised, such as from Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterisation of Thermophilic Bacillus licheniformis SUNGC2 as Producer of α-Amylase from Malaysian Hot Spring

Msarah¹,

Firdose²,

Ibrahim³

et al. 2020

JST

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Screening of new source of novel and industrially useful enzymes is a key research pursuit in enzyme biotechnology. The study aims to report the characteristics of novel thermophilic microorganisms isolated from Sungai Klah (SK) Hot Spring, Perak, Malaysia, that can produce α-amylase. The morphological and biochemical properties were examined for SUNGC2 sample. The isolate was further screened for amylase, followed by 16S rRNA and analytical profile index (API) test. This isolate was further subjected to pH optimisation for α-amylase production. It was found that SUNGC2 was an α-amylase producer and was identified as Bacillus licheniformis SUNGC2 with NCBI accession numbers MH062901. The enzyme was found to exhibit an optimum temperature of 50°C and a pH of 7.0. The relative activity of the enzyme was obtained based on the improvement of the culture conditions. The highest amount of amylase production was 24.65 U/mL at pH 7.0, consecutively the growth was also highest at pH 7.0 with a 9.45-fold increase in specific activity by ammonium phosphate precipitation of 80% (w/v). The results showed that the bacteria isolated from the hot spring are a significant source of thermophilic enzymes that are highly promising in biotechnology.

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Enhanced halophilic lipase secretion by Marinobacter litoralis SW‐45 and its potential fatty acid esters release

et al. 2018

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An approach was made to enhance the halophilic lipase secretion by a newly isolated moderate halophilic Marinobacter litoralis SW‐45, through the statistical optimization of Plackett‐Burman (PB) experimental design and the Face Centered Central Composite Design (FCCCD). Initially, PB statistical design was used to screen the medium components and process parameters, while the One‐factor‐at‐a‐time technique was availed to find the optimum level of significant parameters. It was found that MgSO4 · 7H2O, NaCl, agitation speed, FeSO4 · 7H2O, yeast extract and KCl positively influence the halophilic lipase production, whereas temperature, carbon source (maltose), inducer (olive oil), inoculum size, and casein‐peptone had a negative effect on enzyme production. The optimum level of halophilic lipase production was obtained at 3.0 g L−1 maltose, 1% (v/v) olive oil, 30 °C growth temperature and 4% inoculum volume (v/v). Further optimization by FCCCD was revealed 1.7 folds improvement in the halophilic lipase production from 0.603 U ml−1 to 1.0307 U ml−1. Functional and biochemical characterizations displayed that the lipase was significantly active and stable in the pH ranges of 7.0–9.5, temperature (30–50 °C), and NaCl concentration (0–21%). The lipase was maximally active at pH 8.0, 12% (w/v) NaCl, and 50 °C temperature. Besides, M. litoralis SW‐45 lipase was found to possess the promising industrial potential to be utilized as a biocatalyst for the esterification.

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Partial Purification and Characterization of a Thermoalkalophilic Lipase Originated from Bacillus atrophaeus FSHM2 and its Application for Ester Synthesis

Cited by 6 publications

References 31 publications

Determination the Lipase Activity of Staphylococcus sp. Strain Isolated from Clinical Specimens

Determination the Lipase Activity of Staphylococcus sp. Strain Isolated from Clinical Specimens

Isolation and Characterisation of Thermophilic Bacillus licheniformis SUNGC2 as Producer of α-Amylase from Malaysian Hot Spring

Enhanced halophilic lipase secretion by Marinobacter litoralis SW‐45 and its potential fatty acid esters release

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