Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments

Abstract

BackgroundFungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As laccase production is highly dependent on medium components and cultural conditions, optimization of the same is essential for efficient product production.ResultsProduction of laccase by fungal strain Marasmiellus palmivorus LA1 under solid state fermentation was optimized by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L8) was designed using Qualitek-4 software to study the interactions and relative influence of the seven selected factors by one factor at a time approach. The optimum condition formulated was temperature (28 °C), pH (5), galactose (0.8%w/v), cupric sulphate (3 mM), inoculum concentration (number of mycelial agar pieces) (6Nos.) and substrate length (0.05 m). Overall yield increase of 17.6 fold was obtained after optimization. Statistical optimization leads to the elimination of an insignificant medium component ammonium dihydrogen phosphate from the process and contributes to a 1.06 fold increase in enzyme production. A final production of 667.4 ± 13 IU/mL laccase activity paves way for the application of this strain for industrial applications.ConclusionStudy optimized lignin degrading laccases from Marasmiellus palmivorus LA1. This laccases can thus be used for further applications in different scales of production after analyzing the properties of the enzyme. Study also confirmed the use of taguchi method for optimizations of product production.

Fungal Laccase Production from Lignocellulosic Agricultural Wastes by Solid-State Fermentation: A Review

“…However, their application in biotechnological processes has been limited because of high production costs resulting from low enzyme activity and low yield. Increasing research attention has been paid to effective laccase production strategies associated with increased activity and reduced cost [9,10].…”

Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora‐L168 and its application in bioremediation of triaryl‐methane dye

“…Appropriate selection of substrate is important factor in enzyme production with SSF technique, which determines the success of the process . The utility of low cost substrate for laccase production was highlighted by various researchers . Agro‐waste which otherwise have little or no economic value can be explored for this purpose.…”

“…38 The utility of low cost substrate for laccase production was highlighted by various researchers. 12,39 Agro-waste which otherwise have little or no economic value can be explored for this purpose. In present investigation, the laccase production using red gram and groundnut was considerably higher 540.87 and 550.15 U/g, respectively.…”

“…Both the methods have their own pros and cons, but SSF is more lucrative; since it can add value to the agro‐industrial residues . Various agricultural substrates/by products such as sugarcane bagasse, wheat straw, orange peels, rice bran, barley, and wheat bran have been successfully used in SSF for laccase production using white‐rot fungi . The laccase production is influenced by several factors including method of cultivation, source and quality of nutrients .…”

“…10 Various agricultural substrates/by products such as sugarcane bagasse, wheat straw, orange peels, rice bran, barley, and wheat bran have been successfully used in SSF for laccase production using white-rot fungi. 11,12 The laccase production is influenced by several factors including method of cultivation, source and quality of nutrients. 1 To enhance commercial laccase production, the optimization of physicochemical parameters is essential.…”