Optimization of implantation in the in-vitro fertilization laboratory

Veiga, Anna; Torelló, M.J.; Boiso, Irene; Sandalinas, M.; Busquets, A.; Calderón, Gloria; Barri, Pedro N.

doi:10.1093/humrep/10.suppl_2.98

Cited by 8 publications

(5 citation statements)

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“…The range of protein concentrations in our samples was: 0.23 mg/mL to 6.11 mg/mL, a 26 fold range. This range may reflect variable dilution of the vaginal fluid and mucus during sample collection and expected variability in protein content due to the many changes that occur in the female cycle in response to hormones as well as differences in the water and ion content [ 29 , 32 – 38 ]. Therefore, protein concentration was used to standardize the samples.…”

Section: Methodsmentioning

confidence: 99%

Impact of Bacterial Vaginosis, as Assessed by Nugent Criteria and Hormonal Status on Glycosidases and Lectin Binding in Cervicovaginal Lavage Samples

et al. 2015

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The objective of this study was to evaluate the impact of hormonal status and bacterial vaginosis (BV) on the glycosidases present and glycosylation changes as assessed by lectin binding to cervicovaginal lavage constituents. Frozen cervicovaginal lavage samples from a completed study examining the impact of reproductive hormones on the physicochemical properties of vaginal fluid were utilized for the present study. In the parent study, 165 women were characterized as having BV, intermediate or normal microflora using the Nugent criteria. The presence of glycosidases in the samples was determined using quantitative 4-methyl-umbelliferone based assays, and glycosylation was assessed using enzyme linked lectin assays (ELLA). Women with BV had elevated sialidase, α-galactosidase, β-galactosidase and α-glucosidase activities compared to intermediate or normal women (P<0.001, 0.003, 0.006 and 0.042 respectively). The amount of sialic acid (Sambucus nigra, P = 0.003) and high mannose (griffithsin, P<0.001) were reduced, as evaluated by lectin binding, in women with BV. When the data were stratified according to hormonal status, α-glucosidase and griffithsin binding were decreased among postmenopausal women (P<0.02) when compared to premenopausal groups. These data suggest that both hormonal status and BV impact the glycosidases and lectin binding sites present in vaginal fluid. The sialidases present at increased levels in women with BV likely reduce the number of sialic acid binding sites. Other enzymes likely reduce griffithsin binding. The alterations in the glycosidase content, high mannose and sialic acid binding sites in the cervicovaginal fluid associated with bacterial vaginosis may impact susceptibility to viruses, such as HIV, that utilize glycans as a portal of entry.

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Section: Methodsmentioning

confidence: 99%

Impact of Bacterial Vaginosis, as Assessed by Nugent Criteria and Hormonal Status on Glycosidases and Lectin Binding in Cervicovaginal Lavage Samples

et al. 2015

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“…The use of sequential media (Ménézo et al, 1998a;Gardner et al, 1998) allows development of the embryo to the blastocyst stage, and blastocyst transfer has proved to be useful for certain groups of patients (Veiga et al, 1995(Veiga et al, , 1999. Culture to the blastocyst stage of PGD untransferable embryos provides information on the developmental capacity of biopsied embryos, the characteristics of blastocysts arising from such embryos and on the confirmation of the diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Confirmation of diagnosis in preimplantation genetic diagnosis (PGD) through blastocyst culture: preliminary experience

et al. 1999

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Three cases of preimplantation genetic diagnosis (PGD) (two for sexing and one for aneuploidy screening) are presented. Embryo biopsy was performed at day 3 and diagnosis was established with fluorescent in situ hybridization (FISH). Embryos not used for replacement were cultured in sequential media for blastocyst development. Blastocyst rate was 39.3 per cent. Confirmations of diagnosis were established with FISH in blastocysts and arrested embryos. Mosaicism was observed in 7/8 blastocysts (mean number of cells analysed: 55.5) and 5/8 arrested embryos. The percentage of abnormal cells was 17.1 per cent for blastocysts and 54 per cent for arrested embryos. Polypoid cells were observed in 4/8 blastocysts. Confirmation of diagnosis at the blastocyst stage is a useful tool in PGD.

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“…Embryos diagnosed as normal females for the chromosomes studied (46 XX, 1818) were replaced in the uterine cavity of the patients, on the evening of day 3, using a Wallace catheter (1816 Edwards-Wallace embryo replacement catheter; Simcare, West Sussex, England), while nontransferable embryos (normal male embryos 46 XY, 1818 and abnormal embryos) were kept in coculture in B2 medium (Lab CCD, Paris, France) with a cell support of Vero cells (29) to evaluate the capacity of laser-biopsied embryos to reach the blastocyst stage. Normal male blastocysts were frozen according to the method described by Menezo and Veiga (30).…”

Section: Methodsmentioning

confidence: 99%