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Boada, Montserrat; Carrera, Marta; Iglesia, C. de la; Sandalinas, M.; Barri, P. N.; Veiga, Anna

doi:10.1023/a:1022548612107

Cited by 84 publications

(6 citation statements)

References 32 publications

(32 reference statements)

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“…Currently, PGS is performed in samples biopsied from polar bodies [ 10 - 13 ], cleavage embryos [ 14 , 15 ] or blastocysts [ 16 , 17 ]. It has been found that blastocysts have less mosaicism than cleavage embryos, thus most laboratories prefer blastocyst biopsy, in which multiple cells from the trophectoderm (TE) are biopsied and used for screening [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Arrested human embryos are more likely to have abnormal chromosomes than developing embryos from women of advanced maternal age

Liang

Xian

et al. 2014

J Ovarian Res

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BackgroundAneuploidy is one of the major factors that result in low efficiency in human infertility treatment by in vitro fertilization (IVF). The development of DNA microarray technology allows for aneuploidy screening by analyzing all 23 pairs of chromosomes in human embryos. All chromosome screening for aneuploidy is more accurate than partial chromosome screening, as errors can occur in any chromosome. Currently, chromosome screening for aneuploidy is performed in developing embryos, mainly blastocysts. It has not been performed in arrested embryos and/or compared between developing embryos and arrested embryos from the same IVF cycle.MethodsThe present study was designed to examine all chromosomes in blastocysts and arrested embryos from the same cycle in patients of advanced maternal ages. Embryos were produced by routine IVF procedures. A total of 90 embryos (45 blastocysts and 45 arrested embryos) from 17 patients were biopsied and analyzed by the Agilent DNA array platform.ResultsIt was found that 50% of the embryos developed to blastocyst stage; however, only 15.6% of the embryos (both blastocyst and arrested) were euploid, and most (84.4%) of the embryos had chromosomal abnormalities. Further analysis indicated that 28.9% of blastocysts were euploid and 71.1% were aneuploid. By contrast, only one (2.2%) arrested embryo was euploid while others (97.8%) were aneuploid. The prevalence of multiple chromosomal abnormalities in the aneuploid embryos was also higher in the arrested embryos than in the blastocysts.ConclusionsThese results indicate that high proportions of human embryos from patients of advanced maternal age are aneuploid, and the arrested embryos are more likely to have abnormal chromosomes than developing embryos.

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Section: Introductionmentioning

confidence: 99%

Arrested human embryos are more likely to have abnormal chromosomes than developing embryos from women of advanced maternal age

Liang

Xian

et al. 2014

J Ovarian Res

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“…Zona drilling using a laser is currently proposed as a technically easier method compared to mechanical and chemical means. Although there are reports that laser drilling does not impair embryonic development to the blastocyst stage and implantation [ 13 , 14 , 33 , 34 ], it has been postulated to pose potential harm to embryos in previous studies [ 31 , 35 , 36 ]. Furthermore, a laser is not cost-effective for IVF laboratories where PGD procedures are not performed frequently.…”

Section: Discussionmentioning

confidence: 99%

Outcomes of preimplantation genetic diagnosis using either zona drilling with acidified Tyrode's solution or partial zona dissection

Kim¹,

Kim²,

Lee³

et al. 2012

Clin Exp Reprod Med

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ObjectiveTo review the outcomes of preimplantation genetic diagnosis (PGD) using zona drilling with acid Tyrode's solution (chemical zona pellucida drilling, chemical ZD) and those of partial zona dissection (PZD).MethodsClinical outcomes of seventy-one couples undergoing 85 PGD cycles from January 2005 to December 2010 were included. Blastocyst formation and the hatching rate, clinical pregnancy rate, ongoing pregnancy rate, implantation rate, and fetal gender ratio of the PZD and chemical ZD groups were compared.ResultsApplication of PZD resulted in a significantly higher rate of clinical pregnancy (40.7% vs. 15.4%, p=0.022), ongoing pregnancy (35.6% vs. 11.5%, p=0.023), and implantation (18.1% vs. 5.7%, p=0.007) compared with chemical ZD. Among non-transferred embryos, the rate of blastocyst formation on day 5 (49.1% vs. 39.5%, p=0.016) and hatching on day 6 (47.2% vs. 26.5%, p<0.001) were also significantly higher in the PZD group.ConclusionThe mechanical zona dissection method showed better outcomes than chemical ZD in terms of the blastocyst development and pregnancy rate. In this study, the fact that chemical ZD was conducted in different period from mechanical method should be considered in interpreting the result.

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“…In countries where the genetic analysis of embryos is prohibited, polar body biopsy [47] (Figure 2b) is the only method of tissue sampling to screen for maternal mutations. If there are no legal restrictions, embryo biopsy at the cleavage stage (blastomere biopsy [48]) or at later stages of preimplantation development (trophectoderm [TE] biopsy [6]) can be performed.…”

Section: Ultrashort Laser Microsurgery Of Preimplantation Embryos For Application In Artmentioning

confidence: 99%

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

Ilina

Ситников

2021

Diagnostics

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Although the use of lasers in medical diagnosis and therapies, as well as in fundamental biomedical research is now almost routine, advanced laser sources and new laser-based methods continue to emerge. Due to the unique ability of ultrashort laser pulses to deposit energy into a microscopic volume in the bulk of a transparent material without disrupting the surrounding tissues, the ultrashort laser-based microsurgery of cells and subcellular components within structurally complex and fragile specimens such as embryos is becoming an important tool in developmental biology and reproductive medicine. In this review, we discuss the mechanisms of ultrashort laser pulse interaction with the matter, advantages of their application for oocyte and preimplantation embryo microsurgery (e.g., for oocyte/blastomere enucleation and embryonic cell fusion), as well as for nonlinear optical microscopy for studying the dynamics of embryonic development and embryo quality assessment. Moreover, we focus on ultrashort laser-based approaches and techniques that are increasingly being applied in the fundamental research and have the potential for successful translation into the IVF (in vitro fertilization) clinics, such as laser-mediated individual embryo labelling and controlled laser-assisted hatching.

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Abstract: These results demonstrate the safety and usefulness of laser methodology in preimplantation genetic diagnosis.

Cited by 84 publications

References 32 publications

Arrested human embryos are more likely to have abnormal chromosomes than developing embryos from women of advanced maternal age

Arrested human embryos are more likely to have abnormal chromosomes than developing embryos from women of advanced maternal age

Outcomes of preimplantation genetic diagnosis using either zona drilling with acidified Tyrode's solution or partial zona dissection

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

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