Optimization of HS-SPME conditions for quantification of 2-acetyl-1-pyrroline and study of other volatiles in Pandanus amaryllifolius Roxb.

Wakte, Kantilal V.; Thengane, Ratnakar J.; Jawali, Narendra; Nadaf, Altafhusain

doi:10.1016/j.foodchem.2009.12.056

Cited by 40 publications

(36 citation statements)

References 25 publications

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“…The 2AP peak was identified by matching 2AP standard as a reference material. Quantification of 2AP from the seeds, germinating and vegetative stages of SMB was done following the method of Mathure et al (2011) and Wakte et al (2010) respectively from our laboratory.…”

Section: Plant Materialsmentioning

confidence: 99%

Identification of aroma volatiles and understanding 2-acetyl-1-pyrroline biosynthetic mechanism in aromatic mung bean (Vigna radiata (L.) Wilczek)

Attar

Hinge

Zanan

et al. 2017

Physiol Mol Biol Plants

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Mung bean having high food value and easily digestible proteins, is one of the socioeconomically important crop of India. Among the varied cultivars, Sona mung is having aroma and hence popularly cultivated in the pockets of Ganga river basin at Bhutnir char village of Malda District in the West Bengal state. In the present study, aroma volatiles with special reference to 2-acetyl-1-pyrroline (2AP) were analyzed using HS-SPME-GCMS from Sona mung bean and compared with non-scented mung bean (PHULE M-9339). 26 volatiles in seeds of Sona mung and 20 in non-scented mung bean were identified, in which 3,7-dimethyl-6-octenal, (2E)-2-decen-1-ol, 2-ethyl-1-dodecanol and 3,5,5-trimethyl-2-cyclohexene-1-one are first time reported. 0.19 ± 0.001 ppm 2AP was recorded in Sona mung seeds whereas it was not detected in nonscented mung bean. PCA analysis indicated that 2AP, octanal, 1 pentanol, decanal, phenylmethanol and 2-nonen-1-ol were the major contributors in the aroma of Sona mung bean. The significantly higher level proline, methylglyoxal and lower level of BADH2 transcript were detected in Sona mung than non-scented mung, suggesting similar 2AP biosynthesis mechanism in Sona mung bean as reported in scented rice, sorghum and soybean.

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Section: Plant Materialsmentioning

confidence: 99%

Identification of aroma volatiles and understanding 2-acetyl-1-pyrroline biosynthetic mechanism in aromatic mung bean (Vigna radiata (L.) Wilczek)

Attar

Hinge

Zanan

et al. 2017

Physiol Mol Biol Plants

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“…The quantity of plant material was varied from 5 mg to 1,000 mg. All the experiments for optimization of HS-SPME conditions from Bassia flowers were performed in triplicate. The carboxen/DVB/PDMS SPME fiber of 1 cm attached to manual holder (Supelco, Bellefonte, PA) was selected for analysis of 2AP based on earlier report (Grimm et al 2001;Wakte et al 2010). The fiber was conditioned as per manufacturer's guidelines and then exposed to headspace of the sample in a 4 ml vial at varied temperature and time regimes as specified above.…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…Quantification of 2AP from different growth stages of flower Quantification of 2AP was done by using standard 2AP calibration curve following the method reported in our earlier report (Wakte et al 2010). Standard 2AP solutions in a concentrations series of 25, 50, 75, 100 and 125 ng/l were prepared in benzene and subjected to HS-SPME-GC …”

Section: Chemicals and Reagentsmentioning

confidence: 99%

Mechanism of 2-acetyl-1-pyrroline biosynthesis in Bassia latifolia Roxb. flowers

Wakte

Kad

Zanan

et al. 2011

Physiol Mol Biol Plants

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The flowers of Bassia latifolia are known to contain 2-acetyl-1-pyrroline (2AP), the compound responsible for pleasant aroma in basmati and other scented rice. Four growth stages of Bassia flowers were identified and 2AP contents were analysed in each stage. It was found that 2AP (3.30 ppm) gets synthesized only in fleshy corolla of mature flowers (fourth stage). The activity of γ-aminobutyraldehyde dehydrogenase (AADH); an enzyme responsible for synthesis of γ-aminobutyricacid (GABA) from γ-aminobutyraldehyde (GABald) was assessed in these four stages. The AADH activity was absent in the fourth stage. It was concluded that ceased activity of AADH in fourth stage flowers leads to the accumulation of γ-aminobutyraldehyde which is cyclised spontaneously to Δ 1 -pyrroline, the key precursor of 2AP. Δ 1 -pyrroline further reacts unenzymatically with methylglyoxal to form 2AP.

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“…These compounds are also found in scented rice (aromatic rice) [10][11][12]. Aside from being a food aroma booster, pandanus also has medical uses as a diuretic, cardio-tonic, and an anti-diabetic [13].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of resistant starch, glycemic index and fortificants content of premix rice coated with various concentrations and types of edible coating materials

Yulianto

Susiati

Adhini

2018

IOP Conf. Ser.: Earth Environ. Sci.

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Abstract. The incidence of diabetes in Indonesia has been increasing year by year. Diets with a low glycemic index and high resistant starch foods can assist diabetics in controlling their blood glucose levels. Diabetics are known to have micro-nutrient deficiencies of chromium, magnesium and vitamin D that can be overcome by consuming parboiled rice fortified by use of a coating method. The fortification of parboiled rice (premix rice) can be achieved by coating with HPMC (hydroxypropyl methyl cellulose), MC (methyl cellulose), CMC (carboxyl methyl cellulose), gum arabic and rice starch. This research aimed to evaluate the levels of resistant starch, glycemic index and fortificants of premix rice coated with different concentrations and types of edible coating materials. This research used completely randomized design, with treatments to the concentrations and the types of edible coating (HPMC, CMC, MC, gum arabic and rice starch). The concentrations of edible coating were 0.15%, 0.2% and 0.25% for cellulose derivative coatings; 25%, 30%, 35% for gum arabic and 2%, 3.5% and 5% for rice starch. This research shows that fortified premix rice coated with various concentrations and types of edible coating materials is high in resistant starch and has a low glycemic index. The coating treatment affects the levels of magnesium and vitamin D, but does not affect the levels of chromium in parboiled rice. The premix rice with a low glycemic index and high nutrient content (chromium, magnesium and vitamin D) was premix rice coated by CMC 0.25% and HPMC 0.25% with glycemic indeces of 39.34 and 38.50, respectively.

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Optimization of HS-SPME conditions for quantification of 2-acetyl-1-pyrroline and study of other volatiles in Pandanus amaryllifolius Roxb.

Cited by 40 publications

References 25 publications

Identification of aroma volatiles and understanding 2-acetyl-1-pyrroline biosynthetic mechanism in aromatic mung bean (Vigna radiata (L.) Wilczek)

Identification of aroma volatiles and understanding 2-acetyl-1-pyrroline biosynthetic mechanism in aromatic mung bean (Vigna radiata (L.) Wilczek)

Mechanism of 2-acetyl-1-pyrroline biosynthesis in Bassia latifolia Roxb. flowers

Evaluation of resistant starch, glycemic index and fortificants content of premix rice coated with various concentrations and types of edible coating materials

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