Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys

Rosoklija, Gorazd; Mancevski, Branislav; Ilievski, Boro; Perera, Tarique D.; Lisanby, Sarah H.; Coplan, Jeremy D.; Duma, A.; Serafimova, Tereza; Dwork, Andrew J.

doi:10.1016/j.jneumeth.2003.06.001

Cited by 42 publications

(48 citation statements)

References 26 publications

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“…The Golgi-Cox method is considered to be one of the most reliable techniques for demonstrating dendritic arborization, with a low interference level from background structures (Buell, 1982;Koyama, 2013). In addition, the method has the advantage in staining various types of neurons compared with other staining methods, and the entire dendritic tree of cortical neurons can be clearly visualized (Rosoklija et al, 2003). However, as with the other staining methods, there is the disadvantage that neurons are randomly stained.…”

Section: Golgi-cox Methodsmentioning

confidence: 99%

“…The neuronal body, axon, and dendrites can be clearly visualized, but tiny structures such as dendritic spines are not clear. The Golgi-Kopsch method can efficiently label long-termfixed brains, up to 50 years after fixation, although it does not provide good labeling of fresh fixed brains (Rosoklija et al, 2003). To enhance Golgi-Kopsch staining, Triton X-100 can be used during the process.…”

Section: Golgi-kopsch Methodsmentioning

confidence: 99%

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Comprehensive Review of Golgi Staining Methods for Nervous Tissue

Kang

Kim

Moon

et al. 2017

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Golgi staining has been modified and developed since Camillo Golgi introduced the black reaction in 1873. This study focuses on the commonly used Golgi staining methods and presents comprehensive data regarding three Golgi staining methods along with their strong and weak points. The Golgi-Cox method uses mercuric chloride for brain tissue impregnation and is a reliable technique for analyzing the complete dendritic tree of cortical neurons. However, specimens tend to shrink during the staining steps. Recent combination of the Golgi-Cox method and immunofluorescence provides additional options for neuroscientists. Rapid Golgi staining requires osmium tetroxide for the postfixation process. It homogenously stains whole structures of neurons and provides their detailed anatomical morphology. This staining is influenced by the age of the specimen, temperature of the laboratory, and duration of each procedure. The Golgi-Kopsch method uses formaldehyde and glutaraldehyde instead of osmium tetroxide and can be used regardless of the age of the specimen and the duration after fixation. This method is suitable for research using human brain fixed for a long time or for specimens obtained from old-aged animals. Selecting a Golgi staining protocol that is appropriate for the specimen type and research purpose is important to achieve best results.

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Section: Golgi-cox Methodsmentioning

confidence: 99%

Section: Golgi-kopsch Methodsmentioning

confidence: 99%

Comprehensive Review of Golgi Staining Methods for Nervous Tissue

Kang

Kim

Moon

et al. 2017

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show abstract

“…However, its major drawbacks are lack of uniform crystallization of the stained particles within the cells (Zhang et al, 2003) and lack of reproducible results (Rosoklija et al, 2003). Also, we did not know why and how neurons and glia could be stained separately.…”

Section: Discussionmentioning

confidence: 99%

Differential staining of glia and neurons by modified Golgi-Cox method

Ranjan

Mallick

2012

Journal of Neuroscience Methods

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“…Es importante destacar que muchos de los recientes trabajos realizados con el método de Golgi corresponden a estudios sobre neuropatología humana o experimental (62)(63)(64)(65)(66)(67). Finalmente, una prueba más de la vigencia de este método de impregnación neuronal es el hecho de que todavía, y por extraño que parezca, se publican modificaciones a la técnica de Golgi (68)(69)(70)(71).…”

Section: Vigencia De La Técnica De Golgiunclassified

La técnica de impregnación argéntica de Golgi. Conmemoración del centenario del premio nobel de Medicina (1906) compartido por Camillo Golgi y Santiago Ramón y Cajal

Torres‐Fernández¹

2006

biomedica

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La técnica de Golgi es un sencillo procedimiento histológico que revela la morfología neuronal completa en tres dimensiones. Este método se fundamenta en la formación de depósitos opacos intracelulares de cromato argéntico, producto de la reacción entre el bicromato de potasio y el nitrato de plata (reacción negra). Camillo Golgi, su descubridor, y Santiago Ramón y Cajal, su principal exponente, recibieron el premio nobel de Medicina y Fisiología en 1906 por su contribución al conocimiento de la estructura del sistema nervioso. Gran parte de sus logros se obtuvieron a través de la aplicación del método de impregnación argéntica. Sin embargo, Golgi y Cajal tenían interpretaciones diferentes sobre la estructura del tejido nervioso. Golgi era defensor de la teoría reticular, la cual proponía que el sistema nervioso estaba conformado por una red de células fusionadas a través de los axones a manera de un sincitio. Por el contrario, la doctrina neuronal, defendida por Cajal, sostenía que las neuronas eran células independientes. También se debe a Golgi y su reazione nera el descubrimiento del organelo celular conocido como 'aparato de Golgi'. La microscopía electrónica confirmó los postulados de la doctrina neuronal, así como la existencia del complejo de Golgi, y contribuyó al resurgimiento de la técnica de impregnación argéntica. Aunque existen métodos modernos de tinción intracelular que revelan imágenes excelentes de la morfología neuronal, la técnica de Golgi se mantiene vigente por ser un método más práctico y menos costoso para el estudio de la morfología normal y patológica de las neuronas.Palabras clave: neuroanatomía, neuronas, técnicas histológicas, aparato de Golgi, historia de la medicina, premio nobel. The Golgi silver impregnation method: commemorating the centennial of the Nobel Prize in Medicine (1906) shared by Camillo Golgi and Santiago Ramón y CajalThe Golgi silver impregnation technique is a simple histological procedure that reveals complete three-dimensional neuron morphology. This method is based in the formation of opaque intracellular deposits of silver chromate obtained by the reaction between potassium dichromate and silver nitrate (black reaction). Camillo Golgi, its discoverer, and Santiago Ramón y Cajal its main exponent, shared the Nobel Prize of Medicine and Physiology in 1906 for their contribution to the knowledge of the nervous system structure, Their successes were largely due to the application of the silver impregnation method. However, Golgi and Cajal had different views on the structure of nervous tissue. According to the Reticular Theory, defended by Golgi, the nervous system was formed by a network of cells connected via axons within a syncytium. In contrast, Cajal defended the Neuron Doctrine which maintained that the neurons were independent cells. In addition, Golgi had used a variant of his "black reaction" to discover the cellular organelle that became known as the Golgi apparatus. Electron microscopy studies confirmed the postulates of the Neuron Doctrine as well as th...

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Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys

Cited by 42 publications

References 26 publications

Comprehensive Review of Golgi Staining Methods for Nervous Tissue

Comprehensive Review of Golgi Staining Methods for Nervous Tissue

Differential staining of glia and neurons by modified Golgi-Cox method

La técnica de impregnación argéntica de Golgi. Conmemoración del centenario del premio nobel de Medicina (1906) compartido por Camillo Golgi y Santiago Ramón y Cajal

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