Optimization of glucose-6-phosphate dehydrogenase releasing from Candida guilliermondii by disruption with glass beads

Gurpilhares, Daniela B.; Hasmann, Francislene A.; Pessoa, Adalberto; Roberto, Inês Conceição

doi:10.1016/j.enzmictec.2005.11.018

Cited by 19 publications

(14 citation statements)

References 16 publications

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“…It must be noted that, though the total amount of ADI released in the present case was marginally better in bath sonication, higher specific activity of ADI was achieved by solid shear method. Nevertheless, the release of intracellular enzymes is also dependent on microorganism type, growth phase, nutrition medium and disruption method …”

Section: Discussionsupporting

confidence: 92%

Combined effect of attrition and ultrasound on the disruption of Pseudomonas putida for the efficient release of arginine deiminase

Patil

Shinde

Dev

et al. 2018

Biotechnology Progress

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Disruption of Pseudomonas putida KT2440 by ultrasound treatment in a bath sonicator, in presence of the glass beads, was carried out for the release of arginine deiminase (ADI) and the results were compared with that of by Dyno-mill. The release of ADI depended mainly on the bead size and cellmass concentration being disrupted in bead mill. Nearly 23 U mL ADI was released when slurry with a cell-mass concentration of 250 g L was disintegrated for 9 min with 80% bead loading (0.25 mm) in Dyno-mill. Marginally higher amount of ADI (24.1 U mL ) was released by the bath sonication of 250 g L cellmass slurry for 30 min with the beads (0.1 mm) and a sonication power of 170 W. The glass beads, suspended along with the cellmass slurry in bath sonicator, efficiently disrupted the microbial cells to release ADI. Variation in the kinetic constants for the performance parameters implied that ADI release and cell disruption kinetics is a function of disruption technique used and the process variables thereof. Estimation of location factor suggested that selective release of ADI can be achieved. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018 © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1185-1194, 2018.

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Section: Discussionsupporting

confidence: 92%

Combined effect of attrition and ultrasound on the disruption of Pseudomonas putida for the efficient release of arginine deiminase

Patil

Shinde

Dev

et al. 2018

Biotechnology Progress

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“…The Wnal suspension (about 3 g l ¡1 ) was mechanically disrupted in centrifuge tubes under vortex stirring using glass beads (; 0.5 mm) in a proportion of 3 ml of cell suspension to 3 ml of glass beads (1:1 v/v), at regular time of 5 min [11]. The disruption period was 1 min separated by 30 s interval in an ice bath.…”

Section: Cell Disruptionmentioning

confidence: 99%

The behavior of key enzymes of xylose metabolism on the xylitol production by Candida guilliermondii grown in hemicellulosic hydrolysate

Gurpilhares

Hasmann

Pessoa

et al. 2008

J Ind Microbiol Biotechnol

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A variety of raw materials have been used in fermentation process. This study shows the use of rice straw hemicellulosic hydrolysate, as the only source of nutrient, to produce high added-value products. In the present work, the activity of the enzymes xylose reductase (XR); xylitol dehydrogenase (XD); and glucose-6-phosphate dehydrogenase (G6PD) during cultivation of Candida guilliermondii on rice straw hemicellulosic hydrolysate was measured and correlated with xylitol production under different pH values (around 4.5 and 7.5) and initial xylose concentration (around 30 and 70 g l(-1)). Independent of the pH value and xylose concentration evaluated, the title of XD remained constant. On the other hand, the volumetric activity of G6PD increased whereas the level of XR decreased when the initial xylose concentration was increased from 30 to 70 g l(-1). The highest values of xylitol productivity (Q (P) approximately 0.40 g l(-1)) and yield factor (Y (P/S) approximately 0.60 g g(-1)) were reached at highest G6PD/XR ratio and lowest XR/XD ratio. These results suggest that NADPH concentrations influence the formation of xylitol more than the activity ratios of the enzymes XR and XD. Thus, an optimal rate between G6PD and XR must be reached in order to optimize the xylitol production.

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“…It can be performed without sophisticated devices and does not require chemical treatments or enzymatic cocktails. This technique is easy, cheap, and rivals electroporation for being the least time consuming methodology, but it is also one of the least efficient, because high quantities of DNA are damaged in the process and the viability of the cells is drastically reduced [2,47].…”

Section: Agitation With Glass Beadsmentioning

confidence: 99%

Genetic Transformation of Cells using Physical Methods

Loske¹

2014

J Genet Syndr Gene Ther

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The production of transgenic cells is a routinely process that allows to insert genes from plants, fungi, viruses, bacteria and even animals into cells. Genetic transformation requires penetration of the transgene through the cell wall. This process is facilitated by biological, chemical or physical methods. We present a short review of the state of the art of physical methods used for genetic transformation. A general panorama of the traditional physical genetic transformation methods like electroporation, biolistic, and agitation with glass beads, vacuum infiltration, silicon carbide whisker, laser microbeams, ultrasound, and the newly promising technique of shock wave-mediated genetic transformation of cells are described. These techniques have been applied to transform cells of bacteria, algae, plants, fungi, and animals. In human cells, genetic transformation is currently used for DNA vaccines, tissue engineering, and cancer therapy.

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Optimization of glucose-6-phosphate dehydrogenase releasing from Candida guilliermondii by disruption with glass beads

Cited by 19 publications

References 16 publications

Combined effect of attrition and ultrasound on the disruption of Pseudomonas putida for the efficient release of arginine deiminase

Combined effect of attrition and ultrasound on the disruption of Pseudomonas putida for the efficient release of arginine deiminase

The behavior of key enzymes of xylose metabolism on the xylitol production by Candida guilliermondii grown in hemicellulosic hydrolysate

Genetic Transformation of Cells using Physical Methods

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