Arginine, one among the 20 most common natural amino acids, has a pivotal role in cellular physiology as it is being involved in numerous cellular metabolic and signaling pathways. Dependence on arginine is diverse for both tumor and normal cells. Because of decreased expression of argininosuccinate synthetase and/or ornithine transcarbamoylase, several types of tumor are auxotrophic for arginine. Deprivation of arginine exploits a significant vulnerability of these tumor cells and leads to their rapid demise. Hence, enzyme-mediated arginine depletion is a potential strategy for the selective destruction of tumor cells. Arginase, arginine deiminase and arginine decarboxylase are potential enzymes that may be used for arginine deprivation therapy. These arginine catabolizing enzymes not only reduce tumor growth but also make them susceptible to concomitantly administered anti-cancer therapeutics. Most of these enzymes are currently under clinical investigations and if successful will potentially be advanced as anti-cancer modalities.
Chiral
amines are valuable constituents of many important pharmaceutical
compounds and their intermediates. It is estimated that ∼40%–45%
of small molecule pharmaceuticals contain chiral amine scaffolds in
their structures. The major challenges encountered in the chemical
synthesis of enantiopure amines are the use of toxic chemicals, the
formation of a large number of byproducts, and multistep syntheses.
To address these limitations, cost-effective biocatalytic methods
are maturing and proving to be credible alternatives for the synthesis
of chiral amines in enantiomerically pure forms. Herein, we report
the recent progress achieved and current perspectives in the enzymatic
synthesis of chiral amines using four important enzymes, i.e., imine
reductases, amine dehydrogenases, monoamine oxidases, and cytochrome
P450s. Applications to the industrial synthesis of chiral amines are
highlighted. Protein engineering approaches, which play a critical
role in improving or altering enzyme activity and substrate scope,
are also addressed, along with the discovery of pioneering enzymatic
activities from nature. This survey of recent work demonstrates that
enzymatic approaches to the synthesis of chiral amines will continue
to be a major focus of research in biocatalytic chemistry in the years
to come.
Chiral amines are important components of 40-45% of small molecule pharmaceuticals and many other industrially important fine chemicals and agrochemicals. Recent advances in synthetic applications of ω-transaminases for the production of chiral amines are reviewed herein. Although a new pool of potential ω-transaminases is being continuously screened and characterized from various microbial strains, their industrial application is limited by factors such as disfavored reaction equilibrium, poor substrate scope, and product inhibition. We present a closer look at recent developments in overcoming these challenges by various reaction engineering approaches. Furthermore, protein engineering techniques, which play a crucial role in improving the substrate scope of these biocatalysts and their operational stability, are also presented. Last, the incorporation of ω-transaminases in multi-enzymatic cascades, which significantly improves their synthetic applicability in the synthesis of complex chemical compounds, is detailed. This analysis of recent advances shows that ω-transaminases will continue to provide an efficient alternative to conventional catalysis for the synthesis of enantiomerically pure amines.
The two main strategies for enzyme
engineering, directed evolution
and rational design, have found widespread applications in improving
the intrinsic activities of proteins. Although numerous advances have
been achieved using these ground-breaking methods, the limited chemical
diversity of the biopolymers, restricted to the 20 canonical amino
acids, hampers creation of novel enzymes that Nature has never made
thus far. To address this, much research has been devoted to expanding
the protein sequence space via chemical modifications and/or incorporation
of noncanonical amino acids (ncAAs). This review provides a balanced
discussion and critical evaluation of the applications, recent advances,
and technical breakthroughs in biocatalysis for three approaches:
(i) chemical modification of cAAs, (ii) incorporation of ncAAs, and
(iii) chemical modification of incorporated ncAAs. Furthermore, the
applications of these approaches and the result on the functional
properties and mechanistic study of the enzymes are extensively reviewed.
We also discuss the design of artificial enzymes and directed evolution
strategies for enzymes with ncAAs incorporated. Finally, we discuss
the current challenges and future perspectives for biocatalysis using
the expanded amino acid alphabet.
Bioplastics are derived from renewable biomass sources, such as vegetable oils, cellulose, and starches. An important and high-performance member of the bioplastic family is Nylon 12. The biosynthesis of ω-amino dodecanoic acid (ω-AmDDA), the monomer of Nylon 12 from vegetable oil derivatives is considered as an alternative to petroleum-based monomer synthesis. In this study, for the production of ω-AmDDA from dodecanoic acid (DDA), the cascade of novel P450 (CYP153A), alcohol dehydrogenase (AlkJ), and ω-transaminase (ω-TA) is developed. The regioselective ω-hydroxylation of 1 mM DDA with near complete conversion (>99%) is achieved using a whole-cell biocatalyst co-expressing CYP153A, ferredoxin reductase and ferredoxin. When the consecutive biotransformation of ω-hydroxy dodecanoic acid (ω-OHDDA) is carried out using a whole-cell biocatalyst co-expressing AlkJ and ω-TA, 1.8 mM ω-OHDDA is converted into ω-AmDDA with 87% conversion in 3 h. Finally, when a one-pot reaction is carried out with 2 mM DDA using both whole-cell systems, 0.6 mM ω-AmDDA is produced after a 5 h reaction. The results demonstrated the scope of the potential cascade reaction of novel CYP153A, AlkJ, and ω-TA for the production of industrially important bioplastic monomers, amino fatty acids, from FFAs.
α,ω-Diols are important monomers widely used for the production of polyesters and polyurethanes. Here, biosynthesis of α,ω-diols (C 8-C 16) from renewable free fatty acids using CYP153A monooxygenase, carboxylic acid reductase, and E. coli endogenous aldehyde reductases is reported. The highest yield of α,ω-diol was achieved for the production of 1,12-dodecanediol. In the nicotinamide adenine dinucleotide phosphate (NADPH) cofactor regeneration system, 5 g/L of 1,12-dodecanediol was synthesized in 24 h reaction from the commercial ω-hydroxy dodecanoic acid. Finally, 1.4 g/L 1,12-dodecanediol was produced in a consecutive approach from dodecanoic acids. The results of this study demonstrated the scope of the potential development of bioprocesses to substitute the petroleum-based products in the polymer industry.
A novel ‘parallel anti-sense’ cascade, employing aldehyde reductase and ω-transaminase, has been reported to produce bioplastic monomers with excellent conversion.
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