Optimization of germination protocol for rare endemic Ajuga vestita Boiss. under in vitro conditions

Abstract

Since the seeds of endemic Ajuga vestita are difficult to germinate naturally they were subjected to in vitro culture. Highest rate of germination was obtained in 1/4 MS medium supplemented with 30 g sucrose in light. Microshoots subcultured in 1/1 MS supplemented with 0.125 mg/l Kn rooted well and transferred to soil with 80% success.

Genetic resources and features of clonal micropropagation of Far Eastern species of Actinidia

“…6-BAP is well-known growth regulator for promoting cell division, cell growth, and differentiation of new shoots and generally used during at the micropropagation stage [22]. For effective clonal micropropagation of representatives of the genus Actinidia, 6-BAP has been previously used by other authors [23,24]. In our study, optimal concentrations of 6-BAP were established for the realization of morphogenetic potential of Far Eastern Actinidia species.…”

“…A lot of research has been done to enhance rooting efficiency of the genus Actinidia representatives. Various auxin types and concentrations (IAA, IBA, and α-napthalene acetic acid) were used for root induction at the rooting stage [3,6,7,23]. In this study, explants were rooted on a ½ QL nutrient medium supplemented with auxins IAA and IBA after 14 days, and when use IAA at a concentration of 0.5 mg L -1 the rootability was the highest (96...100%, respectively).…”