Optimization of Freeform Reversible Embedding of Suspended Hydrogel Microspheres for Substantially Improved Three-Dimensional Bioprinting Capabilities

Wu, Catherine A.; Zhu, Yuanjia; Venkatesh, Akshay; Lee, Seung Hyun; Woo, Y. Joseph

doi:10.1089/ten.tec.2022.0214

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…This method offers potential for fabricating complex structures, high construct fidelity, tunable mechanical properties to create a suture-able tissue, reinforcing cell survival through indirect extrusion-based bioprinting, reduced influence bio-inks' rheological properties attributed to FRESH's compatibility with lower viscosity bio-inks, and low costs [5]. However, drawbacks include print repeatability and precision [5], structure integrity and cell viability jeopardized by mechanical forces required for FRESH removal [8], and the impacts of FRESH support bath conditions, which have been previously studied in our work [117].…”

Section: Extrusion-based Bioprintingmentioning

confidence: 88%

Advances in 3D Bioprinting: Techniques, Applications, and Future Directions for Cardiac Tissue Engineering

Zhu

Woo

2023

Bioengineering

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Cardiovascular diseases are the leading cause of morbidity and mortality in the United States. Cardiac tissue engineering is a direction in regenerative medicine that aims to repair various heart defects with the long-term goal of artificially rebuilding a full-scale organ that matches its native structure and function. Three-dimensional (3D) bioprinting offers promising applications through its layer-by-layer biomaterial deposition using different techniques and bio-inks. In this review, we will introduce cardiac tissue engineering, 3D bioprinting processes, bioprinting techniques, bio-ink materials, areas of limitation, and the latest applications of this technology, alongside its future directions for further innovation.

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Section: Extrusion-based Bioprintingmentioning

confidence: 88%

Advances in 3D Bioprinting: Techniques, Applications, and Future Directions for Cardiac Tissue Engineering

Zhu

Woo

2023

Bioengineering

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“…One challenge of embedded bioprinting is the one associated with the properties of the supportive medium. In addition to being non-toxic, biocompatible, non-permeable to the bioink and its components to minimize diffusion, the supporting medium must also possess the appropriate rheological properties, as characterized by yield stress and viscosity, to allow for the movement of the needle through the medium during printing while also being strong enough to support the printed construct without movement after printing [ 364 , 369 ]. In some cases, the support medium may also support the cross-linking of the construct [ 367 ].…”

Section: Key Issues and Future Advances In Extrusion Bioprintingmentioning

confidence: 99%

Biomaterials / bioinks and extrusion bioprinting

Chen,

Fazel Anvari-Yazdi,

Duan

et al. 2023

Bioactive Materials

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“…Shiwarski et al [ 153 ] summarized the current achievements of the emerging 3D bioprinting method called freeform reversible embedding of suspended hydrogels (FRESH) 3D printing. Using this technology, the bioink can be extruded within a thermo-reversible support bath composed of a gelatin microparticle slurry that provides support during printing and is then melted at 37 °C [ 154 , 155 , 156 ]. Although this solution supports 3D soft hydrogel bioprinting, it is difficult to match the kinetics of gelatin dissolution and hydrogel crosslinking [ 150 ].…”

Section: Challenges Of Naturally Derived Bioinks: Mechanical Propertiesmentioning

confidence: 99%

Three-Dimensional Bioprinting of Naturally Derived Hydrogels for the Production of Biomimetic Living Tissues: Benefits and Challenges

Merotto,

Pavan,

Piccoli

2023

Biomedicines

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Three-dimensional bioprinting is the process of manipulating cell-laden bioinks to fabricate living structures. Three-dimensional bioprinting techniques have brought considerable innovation in biomedicine, especially in the field of tissue engineering, allowing the production of 3D organ and tissue models for in vivo transplantation purposes or for in-depth and precise in vitro analyses. Naturally derived hydrogels, especially those obtained from the decellularization of biological tissues, are promising bioinks for 3D printing purposes, as they present the best biocompatibility characteristics. Despite this, many natural hydrogels do not possess the necessary mechanical properties to allow a simple and immediate application in the 3D printing process. In this review, we focus on the bioactive and mechanical characteristics that natural hydrogels may possess to allow efficient production of organs and tissues for biomedical applications, emphasizing the reinforcement techniques to improve their biomechanical properties.

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Optimization of Freeform Reversible Embedding of Suspended Hydrogel Microspheres for Substantially Improved Three-Dimensional Bioprinting Capabilities

Cited by 5 publications

References 38 publications

Advances in 3D Bioprinting: Techniques, Applications, and Future Directions for Cardiac Tissue Engineering

Advances in 3D Bioprinting: Techniques, Applications, and Future Directions for Cardiac Tissue Engineering

Biomaterials / bioinks and extrusion bioprinting

Three-Dimensional Bioprinting of Naturally Derived Hydrogels for the Production of Biomimetic Living Tissues: Benefits and Challenges

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