Biomaterials / bioinks and extrusion bioprinting

Chen, X.B.; Fazel Anvari-Yazdi, A.; Duan, X.; Zimmerling, A.; Gharraei, R.; Sharma, N.K.; Sweilem, S.; Ning, L.

doi:10.1016/j.bioactmat.2023.06.006

Cited by 21 publications

(19 citation statements)

References 387 publications

(453 reference statements)

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“…11 The mechanical-based system offers better control in the deposition of bioinks than the pneumatic-based system. 56 The screw-driven approach is particularly preferred with viscous materials. 11 Another important advantage of the extrusion technique is that it can be used with hydrogels containing high cell density.…”

Section: Bioprinting Methodsmentioning

confidence: 99%

“…Neural tissues can be created utilizing various types of bioprinting methods which are extrusion-based bioprinting (EBB), light-based bioprinting, and droplet-based (inkjet) bioprinting. , Extrusion bioprinting is the most commonly employed bioprinting method as it has an affordable cost, can be easily installed, and allows one to design constructs using desired bioinks with high density. , EBB creates 3D constructs by discharging small quantities of bioinks by sheer force through a nozzle or multinozzle. The mechanical means used in the extrusion process can be categorized as pneumatic-based, screw-driven, and piston-driven systems . Widely used pneumatic-based bioprinting can be controlled easily by altering the pressure.…”

Section: Key Parametersmentioning

confidence: 99%

“…The mechanical-based system offers better control in the deposition of bioinks than the pneumatic-based system . The screw-driven approach is particularly preferred with viscous materials .…”

Section: Key Parametersmentioning

confidence: 99%

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Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Ozbek,

Saybasili,

Ulgen

2024

ACS Biomater. Sci. Eng.

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Primary brain tumor is one of the most fatal diseases. The most malignant type among them, glioblastoma (GBM), has low survival rates. Standard treatments reduce the life quality of patients due to serious side effects. Tumor aggressiveness and the unique structure of the brain render the removal of tumors and the development of new therapies challenging. To elucidate the characteristics of brain tumors and examine their response to drugs, realistic systems that mimic the tumor environment and cellular crosstalk are desperately needed. In the past decade, 3D GBM models have been presented as excellent platforms as they allowed the investigation of the phenotypes of GBM and testing innovative therapeutic strategies. In that scope, 3D bioprinting technology offers utilities such as fabricating realistic 3D bioprinted structures in a layer-by-layer manner and precisely controlled deposition of materials and cells, and they can be integrated with other technologies like the microfluidics approach. This Review covers studies that investigated 3D bioprinted brain tumor models, especially GBM using 3D bioprinting techniques and essential parameters that affect the result and quality of the study like frequently used cells, the type and physical characteristics of hydrogel, bioprinting conditions, cross-linking methods, and characterization techniques.

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Section: Bioprinting Methodsmentioning

confidence: 99%

Section: Key Parametersmentioning

confidence: 99%

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Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Ozbek,

Saybasili,

Ulgen

2024

ACS Biomater. Sci. Eng.

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“…As demonstrated, cells in a cast construct with perfusable channels indeed show improved cell viability and metabolic activity within the proximity of the channels. , Despite their demonstrated potential for vitalizing encapsulated cells, both strategies suffer from significant limitations. For example, many hydrogels used in the bioprinting of vascular channels lack the adequate mechanical strength and stability to support loads or tissue growth before channels inevitably collapse from material degradation. − Inclusion of other materials, such as polymeric fiber fragments, carbon nanotubes, or hydroxyapatite particles into hydrogels, can partially improve the mechanical stability and can be physically blended without involving cytotoxic solvents. − However, these additives may affect printability, and the degradation byproducts need to be considered for their cytocompatibility. In addition, only a few select materials (e.g., sugar) can be adopted for template-based channel formation without harming cells. − Solvents used for template dissolution can often damage cells or lead to high localized concentrations of template material byproducts.…”

Section: Introductionmentioning

confidence: 99%

Creation of Porous, Perfusable Microtubular Networks for Improved Cell Viability in Volumetric Hydrogels

Buckley,

Wang,

O’Dell

et al. 2024

ACS Appl. Mater. Interfaces

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The creation of large, volumetric tissue-engineered constructs has long been hindered due to the lack of effective vascularization strategies. Recently, 3D printing has emerged as a viable approach to creating vascular structures; however, its application is limited. Here, we present a simple and controllable technique to produce porous, free-standing, perfusable tubular networks from sacrificial templates of polyelectrolyte complex and coatings of saltcontaining citrate-based elastomer poly(1,8-octanediol-co-citrate) (POC). As demonstrated, fully perfusable and interconnected POC tubular networks with channel diameters ranging from 100 to 400 μm were created. Incorporating NaCl particulates into the POC coating enabled the formation of micropores (∼19 μm in diameter) in the tubular wall upon particulate leaching to increase the cross-wall fluid transport. Casting and cross-linking gelatin methacrylate (GelMA) suspended with human osteoblasts over the free-standing porous POC tubular networks led to the fabrication of 3D cell-encapsulated constructs. Compared to the constructs without POC tubular networks, those with either solid or porous wall tubular networks exhibited a significant increase in cell viability and proliferation along with healthy cell morphology, particularly those with porous networks. Taken together, the sacrificial template-assisted approach is effective to fabricate tubular networks with controllable channel diameter and patency, which can be easily incorporated into cell-encapsulated hydrogels or used as tissue-engineering scaffolds to improve cell viability.

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“…[ 16–19 ] But cells enclosed within bioinks face risks of compromised viability, activity and DNA stability from unavoidable mechanical stress and free radical during printing and molding, particularly for delicate cell types like stem cells and neuronal cells. [ 20–23 ] Overcoming this challenge is critical to enable translational applications. Many cell‐friendly processes and parameters were established to minimize the cellular damage during fabrication, [ 24,25 ] however, it may require more improvements in techniques for practical use.…”

Section: Introductionmentioning

confidence: 99%

A 3D‐Printed Dual Driving Forces Scaffold with Self‐Promoted Cell Absorption for Spinal Cord Injury Repair

Qiu,

Sun,

et al. 2023

Advanced Science

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Stem cells play critical roles in cell therapies and tissue engineering for nerve repair. However, achieving effective delivery of high cell density remains a challenge. Here, a novel cell delivery platform termed the hyper expansion scaffold (HES) is developed to enable high cell loading. HES facilitated self‐promoted and efficient cell absorption via a dual driving force model. In vitro tests revealed that the HES rapidly expanded 80‐fold in size upon absorbing 2.6 million human amniotic epithelial stem cells (hAESCs) within 2 min, representing over a 400% increase in loading capacity versus controls. This enhanced uptake benefited from macroscopic swelling forces as well as microscale capillary action. In spinal cord injury (SCI) rats, HES–hAESCs promoted functional recovery and axonal projection by reducing neuroinflammation and improving the neurotrophic microenvironment surrounding the lesions. In summary, the dual driving forces model provides a new rationale for engineering hydrogel scaffolds to facilitate self‐promoted cell absorption. The HES platform demonstrates great potential as a powerful and efficient vehicle for delivering high densities of hAESCs to promote clinical treatment and repair of SCI.

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Biomaterials / bioinks and extrusion bioprinting

Cited by 21 publications

References 387 publications

Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Creation of Porous, Perfusable Microtubular Networks for Improved Cell Viability in Volumetric Hydrogels

A 3D‐Printed Dual Driving Forces Scaffold with Self‐Promoted Cell Absorption for Spinal Cord Injury Repair

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