Optimization of Fermentation Parameters for Higher Lovastatin Production in Red Mold Rice through Co-culture of Monascus purpureus and Monascus ruber

Panda, Bibhu Prasad; Javed, Saleem; Ali, Mazhar

doi:10.1007/s11947-008-0072-z

Cited by 79 publications

(44 citation statements)

References 20 publications

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“…MnSO 4 .7H 2 O, glucose and peptone were found more significant medium components for lovastatin yield with Monascus purpureus (Subhagar et al 2010). Panda et al (2010b) had attempted screening of the nutrients with Plackett-Burman design for lovastatin production and concluded that it was greatly influenced by the concentration of malt extract, (Lopez et al 2003). According to the literature, widely used carbon sources for lovastatin production are lactose, glycerol and glucose.…”

Section: Screening Of Substrates and Nutrientsmentioning

confidence: 99%

Bio-synthesis and screening of nutrients for lovastatin by Monascus sp. under solid-state fermentation

Dikshit

Tallapragada

2015

J Food Sci Technol

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In this study Monascus strains were screened for lovastatin production. These strains namely Monascus purpureus, Monascus sanguineus and their co-culture were able to produce lovastatin in solid state fermentation. Sensitivity of lovastatin was tested on Saccharomycess cerevaceae and Candida sp. where the former exhibited large zone of inhibition as compared to the latter. Presence of lovastatin was confirmed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Quantification of lovastatin was done with UV spectrometer at 238 nm. Further, Plackett-Burman methodology was applied for screening of nutrients for lovastatin production. Different substrates were screened and amongst them, wheat bran was found to be the best substrate for lovastatin synthesis. Seven nutrients were screened according to the Plackett-Burman design for lovastatin yield. MgSO 4 .7H 2 O showed the positive impact on lovastatin yield whereas lactose showed the maximum negative effect with M. purpureus. For M. sanguineus, CaCl 2 .2H 2 O displayed the dominant negative effect and soybean the significant positive. With co-culture, the effect of lactose was positive whereas that of malt extract was negative and dominant. The maximum lovastatin yield for M. sanguineus, M. purpureus and co-culture was estimated to be 0.402, 0.27 and 0.26 mg/g respectively.

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Section: Screening Of Substrates and Nutrientsmentioning

confidence: 99%

Bio-synthesis and screening of nutrients for lovastatin by Monascus sp. under solid-state fermentation

Dikshit

Tallapragada

2015

J Food Sci Technol

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“…In the former case, monacolin K was found to inhibit cholesterol synthesis thus reducing hypolipidemia (Chairote et al 2008;Lee et al 2007), and lovastatin was found to reduce serum cholesterol and triglyceride (Panda et al 2009). The red pigment has been of increasing interest to the food industry because products are extracellular and water soluble making them easy to use.…”

Section: Introductionmentioning

confidence: 99%

Improving Solid-State Fermentation of Monascus purpureus on Agricultural Products for Pigment Production

Nimnoi

Lumyong

2009

Food Bioprocess Technol

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In the present study, the effects of culture medium and temperature on red pigment production and mycelia growth were evaluated. The maximum red pigment production was found when Monascus purpureus CMU001 was cultivated on potato dextrose broth at 30°C for 2 weeks. The highest amount of dry weight was achieved when cultivated on tryptone glucose yeast extract medium. Cheap agricultural products and residues were used as substrates for pigment production. Corn meal was the best substrate for pigment production (19.4 U/gds) when compared to peanut meal, coconut residue, and soybean meal. The highest pigment yield (129.63 U/gds) was found when corn meal was supplemented with 8% (w/w) glucose, followed by coconut residue (63.50 U/gds), peanut meal (52.50 U/gds), and soybean meal (22.50 U/gds). Galactose, sorbose, psicose, and mannitol were found to be good supplements next to glucose but not xylitol. Pigment was not stable at high temperature and long exposure to UV. The intensity of red pigment decayed 30.57% and 5.41% after autoclaving and pasteurization, respectively.

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“…A maximum lovastatin production of 2.83 mg/g fungal dry weight was obtained [18]. Effect of different substrates (sweet potato, potato, cassava and dioscorea) on monacolin-K production was investigated.…”

Section: Introductionmentioning

confidence: 99%

Effect of media composition on citrinin and bio-pigments production by Monascus ruber

2017

J App Biol Biotech

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Monascus species are used for production of red bio-pigments, which are used as food coloring agents. However, presence of the mycotoxin citrinin, which is a secondary metabolite produced in conjunction with the red pigments, prevents the use of these pigments in large scale. In the present study, Monascus ruber Van Tieghem was isolated from the Egyptian soil and identified. Effect of media composition on nine of the pigments produced by this fungus in addition to citrinin was studied. The fungus was grown in four different media containing different carbon sources (glucose, ethanol, yeast malt extract and potato dextrose broth). On the other hand, effect of glucose concentration was also studied. The produced pigments and citrinin were extracted by butanol and analyzed by LC/MS/MS as well as HPLC with fluorescence detection of citrinin. The data revealed that the highest amount of red pigments production (4.3g/l) was obtained at glucose concentration 10g/l. This was combined with complete absence of citrinin. Rubropunctamine (26.1%) and ankuflavine (48.7%) were the most abundant pigments in the glucose and ethanol media, respectively; while Moanascin (61.6%) and N-glutarylrubropunctamine (39.4%) were the most abundant in the PDB and YMB media, respectively. This result enables the large scale production of the red pigments by M. ruber without production of the mycotoxin citrinin.

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Optimization of Fermentation Parameters for Higher Lovastatin Production in Red Mold Rice through Co-culture of Monascus purpureus and Monascus ruber

Cited by 79 publications

References 20 publications

Bio-synthesis and screening of nutrients for lovastatin by Monascus sp. under solid-state fermentation

Bio-synthesis and screening of nutrients for lovastatin by Monascus sp. under solid-state fermentation

Improving Solid-State Fermentation of Monascus purpureus on Agricultural Products for Pigment Production

Effect of media composition on citrinin and bio-pigments production by Monascus ruber

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