Optimization of extraction techniques and quantification of Betulinic Acid (BA) by RP-HPLC method from Ancistrocladus heyneanus Wall. Ex Grah.

Pai, Sandeep R.; Nimbalkar, Mansingraj S.; Pawar, Nilesh V.; Dixit, G. B.

doi:10.1016/j.indcrop.2011.05.006

Cited by 40 publications

(24 citation statements)

References 17 publications

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“…Two major parameters affecting content yield of analytes, includes method of extraction and time required for it. There are a number of methods identified for extraction of plant based activities and metabolites (Pai et al, 2011a(Pai et al, ,b, 2015Murugan and Parimelazhagan, 2014;Nimbalkar et al, 2012;Patil et al, 2012;Pawar et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Upadhya

Pai

Hegde

2015

Journal of King Saud University - Science

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The main purpose of this study was to evaluate the effect of extraction method with respect to time of exposure on total phenolic content and antioxidant potential of methanolic extracts (95%) from Achyranthes aspera leaves, stem and roots. Total phenolic content (TPC) was determined using the Folin-Ciocalteu method and antioxidant potential was tested using DPPH radical scavenging and FRAP assays. Plant populations aged 60 and 120 days were considered during the study. Overall, highest TPC was evident in mature plants (120 days) compared to younger (60 days). Similarly, leaves accumulated higher phenolics followed by roots and stem. Results showed the MAE technique to be efficient over USE and CSE methods. Same was evident in both the antioxidant assays tested. A similar trend was observed in both antioxidant assays as that of TPC, indicating phenolics to be the major contributor in the antioxidant potential of the plant. In conclusion it can be said that the yield of phenolic compounds depends on parameters viz. age of plant, part used for extraction, method of extraction and time required for the same. ª 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Section: Introductionmentioning

confidence: 99%

Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Upadhya

Pai

Hegde

2015

Journal of King Saud University - Science

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“…The therapeutic use of the plant is principally attributed to the presence of biomarkers (Shailajan et al, 2015) of which betulinic acid, pentacyclic triterpenoid (Figure 1), is one of the bioactive phytoconstituents betulinic acid has a wide range of biological and medicinal properties, including anti-human immunodeficiency virus (HIV), antibacterial, antimalarial, anti-inflammatory, antihelmintic, antinociceptive, anti-herpes simplex viruses-1 (HSV-1), immunemodulatory, antiangiogenic, and anticancer activity (Sook et al, 2015). Literature survey reveals that various chromatographic methods are available for quantitative estimation of betulinic acid from different plants (Pai et al, 2011;Mukherjee et al, 2010). However, there are few HPTLC and rapid HPLC methods available for analysis of betulinic acid from S. racemosa bark and traditional formulations.…”

Section: Introductionmentioning

confidence: 99%

Application of modern RP-HPLC technique for the quantitation of betulinic acid from traditional drug Symplocos racemosa Roxb.

2017

J App Pharma Sci

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Objective: Symplocos racemosa Roxb. is traditionally used in various ailments like leprosy, diarrhoea, dysentery, menorrhagia and uterine disorders etc. The plant exhibits these therapeutic effects due to the presence of different biologically active phytoconstituents like ellagic acid, symplocoside, betulin, oleanolic acid, β-sitosterol, betulinic acid, etc. The bark has been reported to possess antiandrogenic, anticancer, antioxidant, antiulcer, hepatoprotective, angiogenic activity, in gynecological disorders etc. Therefore, the bark of S. racemosa has been an important ingredient of many traditional and Ayurvedic formulations like Rodhrasava, Pushyanug churna, Dashmularista, etc. The present study aims to develop and validate an efficient RP-HPLC method for the quantification of betulinic acid from the bark of Symplocos racemosa and an Ayurvedic formulation. Material and Methods: Separation was achieved on C18-column using acetonitrile: distilled water (85:15, v/v) as the mobile phase at a flow rate of 1 mL/min. The detection was carried out at 210 nm. Result and Conclusion: The linearity range was 5-150 µg/mL. The content of betulinic acid in Pushyanug churna was found to be 2.55±0.039 mg/g. The validated method was found to be simple, sensitive, accurate, rugged and reproducible. The developed method can be recommended for marker-based standardization of traditional formulations containing bark of S. racemosa.

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“…The use of UAE to recover triterpenic acids from different plant matrix has been recently studied [18][19][20] and has proved to present several advantages in comparison with conventional solid-liquid extraction. These advantages include reduction of the amount of solvent required, time and temperature, which represents an important factor when extracting thermolabile compounds [21].…”

Section: Introductionmentioning

confidence: 99%

Recovery of betulinic acid from plane tree (Platanus acerifolia L.)

Pinilla

López-Padilla

Vicente

et al. 2014

The Journal of Supercritical Fluids

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Betulinic acid (3β, hydroxy-lup-20(29)-en-28-oic acid) is a bioactive triterpenic acid which was identified in various botanical sources and in considerable amounts in the bark of plane tree (Platanus acerifolia L.). In this work, the recovery of betulinic acid from plane tree bark was studied using different liquid solvent based extraction methods, namely Solid-Liquid Extraction (SLE), Ultrasound Assisted Extraction (UAE) and Pressurized Liquid Extraction (PLE). Furthermore, preliminary studies of the Supercritical Fluid Extraction (SFE) of plane tree bark are also reported.The liquid solvent based extraction techniques (SLE, UAE and PLE) were carried out using ethanol and ethyl acetate, and produced a recovery of betulinic acid in the range 10-15 mg per g of bark, with concentrations around 25-35 % mass. A betulinic acid enrichment in the ethanolic extracts was possible by means of a simple precipitation step adding water. The precipitate contained 42-46 % mass of betulinic acid and high 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 recovery (> 95%). Increasing the extraction temperature, by means of the PLE assays, has not resulted in an improvement of betulinic acid recovery. Manuscript Click here to view linked ReferencesThe preliminary SFE assays produced lower recoveries of betulinic acid (0.5-8 mg/g) with respect to liquid extraction. The addition of ethanol as cosolvent produced a significant improvement of both betulinic acid recovery and concentration in the SFE extract.

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Optimization of extraction techniques and quantification of Betulinic Acid (BA) by RP-HPLC method from Ancistrocladus heyneanus Wall. Ex Grah.

Cited by 40 publications

References 17 publications

Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Application of modern RP-HPLC technique for the quantitation of betulinic acid from traditional drug Symplocos racemosa Roxb.

Recovery of betulinic acid from plane tree (Platanus acerifolia L.)

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