Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components

Abstract

By using extraction yield, total polyphenolic content, antidiabetic activities (PTP-1B and α-glycosidase), and antioxidant activity (ABTS and DPPH) as indicated markers, the extraction conditions of the prescription Kursi Wufarikun Ziyabit (KWZ) were optimized by response surface methodology (RSM). Independent variables were ethanol concentration, extraction temperature, solid-to-solvent ratio, and extraction time. The result of RSM analysis showed that the four variables investigated have a significant effect (p < 0.05) for Y1, Y2, Y3, Y4, and Y5 with R2 value of 0.9120, 0.9793, 0.9076, 0.9125, and 0.9709, respectively. Optimal conditions for the highest extraction yield of 39.28%, PTP-1B inhibition rate of 86.21%, α-glycosidase enzymes inhibition rate of 96.56%, and ABTS inhibition rate of 77.38% were derived at ethanol concentration 50.11%, extraction temperature 72.06°C, solid-to-solvent ratio 1 : 22.73 g/mL, and extraction time 2.93 h. On the basis of total polyphenol content of 48.44% in this optimal condition, the quantitative analysis of effective part of KWZ was characterized via UPLC method, 12 main components were identified by standard compounds, and all of them have shown good regression within the test ranges and the total content of them was 11.18%.

Optimization of Extraction Conditions of Phytochemical Compounds and Anti‐Gout Activity of Euphorbia hirta L. (Ara Tanah) Using Response Surface Methodology and Liquid Chromatography‐Mass Spectrometry (LC‐MS) Analysis

“…As shown in Figure 3(a), XO inhibitory activity Evidence-Based Complementary and Alternative Medicine increased when the temperature increased from 50 to 75°C and started to decrease towards 100°C as the extraction time decreased. is was in agreement with Edirs et al [46] where the antidiabetic activity of Kursi Wufarikun Ziyabit using protein tyrosine phosphatase-1B and α-glucosidase inhibition assays increased as the extraction temperature increased from 70 to 75°C and 65 to 70°C, respectively. is explains that an increase in the extraction temperature beyond certain value will denature or decompose some of the valuable compounds responsible for inhibiting XO activity [47].…”

Kursi Wufarikun Ziyabit Improves the Physiological Changes by Regulating Endoplasmic Reticulum Stress in the Type 2 Diabetes db/db Mice

“…Then, our results demonstrated that KWZ successfully induced the HK, which is consistent with the increase of insulin signaling activity. KWZ is rich in antioxidant components, and the previous study gave evidence of the antioxidant activity of the effective part [ 21 ]. Diabetes is related to oxidative stress, marked by increased production of free radicals or impaired antioxidant defenses [ 35 , 36 ].…”

“…Voucher specimens (Barcode: G. collinum WY01053, H. Scabrum WY01054) were deposited at the Herbarium of the Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences. e KWZ (the roots of G. collinum and aerial parts of H. Scabrum were mixed with the ratio of 7 : 3) were prepared according to the article [21]. e dried matter after preparation was powdered, weighed, and packed in zip pack bags, stored at 4°C for further study.…”

“…We have referred to the above report messages, which previously found the effective part of two herbs, named as Kursi Wufarikun Ziyabit (KWZ). Firstly, we optimized the best proportion and extraction conditions, identified the main components, and detected the antidiabetic activities by protein tyrosine phosphatase-1B (PTP-1B) and α -glucosidase inhibitors [ 21 ]. Then, the hypoglycemic effect of KWZ on L6 rat skeletal muscle cells and its mechanism was investigated, mainly including the glucose consumption, treatment time, and the expression of the major proteins in insulin resistance and ER stress signaling pathways [ 22 ].…”

“…We have referred to the above report messages, which previously found the effective part of two herbs, named as Kursi Wufarikun Ziyabit (KWZ). Firstly, we optimized the best proportion and extraction conditions, identified the main components, and detected the antidiabetic activities by protein tyrosine phosphatase-1B (PTP-1B) and α-glucosidase inhibitors [21].…”