Optimization of extraction conditions of Corni Fructus for functional materialization

Abstract

In order to determine a proper extraction condition of Corni Fructus, antioxidant capacities and Hunter's colors values of Corni Fructus powder (CFP) were evaluated as functions of the particle size, extraction solvents and extraction time. The antioxidant capacity was determined in terms of the total phenolic contents (TPC), total anthocyanin contents (TAC), and DPPH radical scavenging activity. CFP was classified as CFP-1 (25-45 mesh), CFP-2 (45-60 mesh), CFP-3 (60-100 mesh), and CFP-4 (>100 mesh). The antioxidant capacities and extraction yield, depending on the particle size of CFP, were the highest for CFP-2, with an extraction yield of 5.45%, a-value of 30.71, TPC of 3.52 μM GAE, TAC of 19.20 mg/L. Depending on the extraction solvent, the extraction yields were the highest to 60% ethanol, at 9.15% (p<0.05); further, antioxidant capacities were that TAC was highest to 90% EtOH with 12.18 μM GAE, whereas DPPH was not significantly different (p<0.05). Depending on the extraction time, extraction yields were highest (4.43%) for extraction in 3 h, and. TAC and DPPH were not significantly different (p<0.05). Therefore, the antioxidant capacity of CFP was excellent with 45-60 mesh (CFP-2), an extraction solvent of 90% EtOH, and extraction time of 3 h. The findings establish the proper extraction conditions of Corni Fructus for its use as a functional material.