A 90% ethanol extract of Corni fructus was fractioned and the active components (i.e., morroniside, loganin) were isolated for functional food materialization. More specifically, the ethanol extract was fractioned according to the solvent polarity (i.e., n-hexane, ethyl acetate, and n-butanol), and the ethyl acetate fraction was found to exhibiti the highest antioxidant capacities (i.e., total phenolic content = 145.84 GAE mg/100 mL, DPPH radical scavenging activity = 7.91 mM GAE, FRAP activity = 18.86 mM TE, ABTS radical scavenging activity = 13.36 mM TE). Diaion HP 20 gel was used for separation of the ethyl acetate fraction, and 20%, 40%, 60%, and 80% ethanol fractions were also examined. The 40% ethanol fraction was found to exhibit the highest DPPH radical scavenging activity (10.22 mM GAE), ABTS radical scavenging activity (37.29 mM TE), and FRAP activity (27.32 mM TE) (p<0.05). The total phenolic compound content (580.84 GAE mg/100 mL), total flavonoid content (166.24 CE mg/100 mL), and total anthocyanin content (0.74 mg/100 mL) followed a similar trend, again being the highest for this fraction. Two components (i.e., morriniside, loganin) were identified in the ethyl acetate and 40% ethanol fractions, and their contents were determined by high-performance liquid chromatography. Furthermore, the 40% ethanol fraction contained the highest quantity of total active components (p<0.05), and the presence of morroniside (405 [M-H]and 451 [M+COOH-H]-) and loganin (389 [M-H]and 435 [M+COOH-H]-) were confirmed by ultra-performance liquid chromatography-mass spectrometry.
To determine the proper picking period for Corni Fructus, the seed removal degree, free sugar contents, antioxidant capacities, and Hunter's color values were investigated at intervals of 15 days from 236 days after full bloom (DAFB) to 327 DAFB. The seed removal degree maximized at 280 DAFB (p<0.05), at this point the moisture contents were 197.40% (db) and 66.00% (wb). The seed removal degrees were also investigated during hot air drying, and the best seed removal degree was obtained at moisture contents of 191.90% (db) and 65.74% (wb), similar to those observed at 280 DAFB. Fructose and glucose were detected, and their contents decreased slowly after 280 DAFB. Total phenolic contents (TPC), and DPPH radical scavening activity both decreased over the picking periods, but total anthocyanin contents (TAC) decreased after 280 DAFB. The redness, and yellowness of Corni Fructus powders, determined by using Hunter's colorimeter, decreased after 280 DAFB, but lightness decreased over the picking periods. Studies of the physicochemical quality characteristics revealed that the proper picking period of Corni Fructus should be 280 DAFB. Key words:seed removal degree, Corni Fructus, picking periods, antioxidant capacity, free sugar contents
초록 우수한 고구마 종순 생산을 위해 식물생장조절제인 BA, kinetin, 2,4-D를 농도별 조합한 처리용액에 서 고구마를 생육시켰다. 연구의 결과, 고구마 종순의 초장 길이는 BA 3 mg·L -1 처리구에서 53.5 cm 로 가장 길었고, 2,4-D 1 mg·L -1 처리구에서 9.00 cm로 가장 짧았다. 고구마 종순의 절수는 BA 3 mg·L -1 처리구에서 20개로 가장 많았고, 2,4-D 1 mg·L -1 처리구에서 9개로 가장 적었다. 고구마 종 순 5엽의 크기는 2,4-D가 첨가된 모든 처리구들에서 대조구보다 작았지만, 엽의 길이는 kinetin 1 mg·L -1 에서 6.00 cm로 다른 처리구보다 길이가 길었고, 엽의 넓이는 kinetin 2 mg·L -1 처리구에서 대조구와 비슷한 수준을 보였다. 고구마 종순의 첫 번째, 세 번째, 다섯 번째 절간들의 직경을 측정했 을 때, 첫 번째 절간에서는 BA 2 mg·L -1 처리구와 BA 2 mg·L -1 과 2,4-D 1 mg·L -1 이 첨가된 혼용 처리구에서 2.4 mm로 가장 두꺼웠고, 세 번째와 다섯 번째 절간들에서는 BA 1 mg·L -1 처리구에서 각각 3.72 mm, 4.32 mm로 가장 두꺼웠다. 따라서 시토키닌류의 식물생장조절제인 BA를 처리했을 때 고구마 종순 생육이 비교적 우수한 것을 확인할 수 있었다.검색어 -고구마, 식물생장조절제, 고구마 종순 ABSTRACT BA, kinetin and 2,4-D were used for increase the yield of sweet potato sapling as plant growth regulators. Sweet potatoes were grown on nine treatments of solution containing different concentration of plant growth regulators. The longest total length of sweet potato sapling was 53.5 cm grown on 3 mg·L -1 BA-treated solution, whereas shortest was 9.00 cm grown on 1 mg·L -1 2,4-D-treated solution. The node number of sapling was 20 grown on 3 mg·L -1 BA-treated solution and 9 grown on 1 mg·L -1 2,4-D-treated solution. The size of fifth leaves of
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