Optimization of ethanol, citric acid, and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger, and Candida guilliermondii

Abstract

The present study deals with submerged ethanol, citric acid, and α-amylase fermentation by Saccharomyces cerevisiae SDB, Aspergillus niger ANSS-B5, and Candida guilliermondii CGL-A10, using date wastes as the basal fermentation medium. The physical and chemical parameters influencing the production of these metabolites were optimized. As for the ethanol production, the optimum yield obtained was 136.00 ± 0.66 g/l under optimum conditions of an incubation period of 72 h, inoculum content of 4% (w/v), sugars concentration of 180.0 g/l, and ammonium phosphate concentration of 1.0 g/l. Concerning citric acid production, the cumulative effect of temperature (30°C), sugars concentration of 150.0 g/l, methanol concentration of 3.0%, initial pH of 3.5, ammonium nitrate concentration of 2.5 g/l, and potassium phosphate concentration of 2.5 g/l during the fermentation process of date wastes syrup did increase the citric acid production to 98.42 ± 1.41 g/l. For the production of α-amylase, the obtained result shows that the presence of starch strongly induces the production of α-amylase with a maximum at 5.0 g/l. Among the various nitrogen sources tested, urea at 5.0 g/l gave the maximum biomass and α-amylase estimated at 5.76 ± 0.56 g/l and 2,304.19 ± 31.08 μmol/l/min, respectively after 72 h incubation at 30°C, with an initial pH of 6.0 and potassium phosphate concentration of 6.0 g/l.

Production of α-Amylase by Aspergillus terreus NCFT 4269.10 Using Pearl Millet and Its Structural Characterization

“…Monga et al (2011) have also reported the use of casein and peptone for the biosynthesis of amylase using various Aspergillus species. Acourene and Ammouche (2012) reported that urea was the most compatible elicitor for biosynthesis of amylase from A. niger which disagrees with the present findings.…”

“…Aspergillus awamori nakazawa was also reported as an effective producer of glucoamylases and proteases in solid state fermentation with wheat bran as substrate (Negi and Banerjee, 2009). Acourene and Ammouche (2012) have produced α-amylase using three fungal strains, Saccharomyces cerevisiae, A. niger , and Candida guilliermondii from date wastes. Roses and Guerra (2009) have optimized production of amylase by A. niger using sugarcane bagasse as the substrate by solid-state fermentation.…”

“…and Rhizopus spp., probably due to their ubiquity and non-pretentious nutritional requirements. Substrates like vegetable waste, rice husk, banana peels (Khan and Yadav, 2011), wheat bran (Negi and Banerjee, 2010), date wastes (Acourene and Ammouche, 2012), sugarcane baggase (Roses and Guerra, 2009), wheat straw, rye straw, corncob leaf, oil cakes, and many others (Bhargav et al, 2008) have been utilized for the biosynthesis of amylases. Consequently, SSF process is of particular economic concern for such countries which have generation of huge plant biomass and agro-industrial wastes.…”

Generation of bioethanol from common date by-products, Teggaza and Lebghel in Southern Algeria

“…Using bioethanol instead of gasoline leads to the reduction of carbon emission by 80% and overall gasoline consumption by more than 30% (Elsanhoty et al, 2012). The production of bioethanol from lignocellulose raw materials requires generally the incorporation of an efficient pretreatment, followed by a scarification of the carbohydrates to obtain satisfactory effectiveness (Acourene and Ammouche, 2012). Compared to the use of gasoline, bioethanol helps to reduce CO 2 emission to about 80% (Li et al, 2008).…”

Citric Acid Production from Sugarcane Bagasse through Solid State Fermentation Method Using Aspergillus niger Mold and Optimization of Citric Acid Production by Taguchi Method

“…On the other hand, the high level of sugarcane bagasse production after the completion of Khuzestan province projects (three million tons per year) and the problems caused due to its storage require a comprehensive measurement in the direction of its optimized utilization and citric acid production (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16).…”