Optimization of Enzymatic Processing of Rapeseed.

Kubaychuk, Oksana; Nosenko, Tamara; Cherstva., Alona

doi:10.21474/ijar01/1634

Cited by 1 publication

(9 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzymatic pretreatmentof rapeseed was carried out by using a mixture of enzyme preparations Protolad and Celuladat the amount from 0.4 to 1.4 % by weight of the substrate. The concentration of enzymes 0.4 % was the most optimal, the oil yield ranged from 32.2 to 45.9 %, depending on other process parameters, such as processing time and moisture content before pressing (109,110).…”

Section: Enzyme:substrate Ratiomentioning

confidence: 99%

“…with proteolytic, cellulolytic and pectolytic activity to improve aqueous extraction, organic solvent extraction and press extraction of the oil (3,4,6,9,10,(109)(110)(111)(112)(113)(114)117,118,130,132,133,137).…”

Section: Temperature Of the Enzymatic Hydrolysismentioning

confidence: 99%

“…Heating to 50-60 °C causes a sharp decrease in its oil viscosity, while with a further increase in temperature, the viscosity changes are less significantly (142). At the same time, a temperature range from 40 to 55 °C is the most optimal for enzymatic pretreatment of oilseeds, at lower temperatures the process will not be efficient enough, and at temperature above 60 °C denaturation of the enzyme protein part and its inactivation will occur (3,4,6,9,10,13,(108)(109)(110)(111)(112)(113)(114)117,118,130,132,133,137).…”

Section: Technical Considerations For Enzymatic Pretreatmentmentioning

confidence: 99%

“…The use of enzymes with different activities has also been investigated for the extraction of vegetable oils from seeds, e.g. a mixture of proteases and cellulases in different ratios and under different conditions in order to increase the yield of pressed oil from pumpkin seed and rapeseed ( 108 - 110 ). Many researchers have treated various oilseeds with mixtures consisting of enzymes with proteolytic, cellulolytic and pectolytic activity ( 6 , 9 , 13 , 111 , 112 ).…”

Section: Combination Of Enzymes Used For Oilseed Pretreatmentmentioning

confidence: 99%

“…Most scientists who developed the technology of enzymatic treatment before pressing of various oilseeds tried to minimize the amount of water added to the substrate during enzymatic hydrolysis ( Fig. 1 ( 3 , 6 , 9 , 108 , 114 , 118 , 130 )), in particular, they reduced the substrate/water mass ratio to 1:1, or to the amount of water relative to substrate 45–50 % ( 3 , 4 , 9 , 10 , 108 - 110 , 119 ). There are also studies where the preliminary enzymatic treatment of oil material was performed at different water amount added to the substrate.…”

Section: Factors Influencing Enzyme Activity and Efficiencymentioning

confidence: 99%

See 4 more Smart Citations

Enzymatic Pretreatment of Plant Cells for Oil Extraction

Vovk

Karnpakdee

Ludwig

et al. 2023

Food Technol. Biotechnol. (Online)

Self Cite

View full text Add to dashboard Cite

Oil from oilseeds can be extracted by mechanical extraction (pressing), aqueous extraction, or by extraction with organic solvents. Although solvent extraction is the most efficient method, organic solvents are a potential hazard to the life and health for workers as well as to the environment, when solvent vapors are released and act as air pollutant with a high ozone forming potential. Pressing is safer, environmentally friendly, and it preserves valuable natural components in the resulting oils. The problems associated with pressing are the high energy consumption and the lower yield on oil extraction, because the applied mechanical force does not completely destroy the structural cell components storing the oil. In seed cells, the oil is contained in the form of lipid bodies (oleosomes) that are surrounded by a phospholipid monolayer with a protein layer on the surface. These lipid bodies are further protected by the seed cell walls consisting mainly of polysaccharides such as pectins, hemicelluloses and cellulose, but also of glycoproteins. The use of hydrolases to degrade these barriers is a promising pretreatment strategy to support mechanical extraction and improve the oil yield. It is advisable to use a combination of enzymes different activities when considering the multicompartment and multicomponent structure of oilseed cells. This article gives an overview on the microstructure and composition of oilseed cells, reviews enzymes capable to destroy oil containing cell compartments, and summarizes the main parameters of enzymatic treatment procedures, such as the composition of the enzyme cocktail, the amount of enzyme and water used, temperature, pH, and the duration. Finally, it analyzes the efficiency of proteolytic, cellulolytic, and pectolytic enzyme pretreatment to increase the yield of mechanically extracted oil from various types of vegetable raw materials with the main focus on oilseeds.

show abstract

Section: Enzyme:substrate Ratiomentioning

confidence: 99%