Optimization of entrapment efficiency and evaluation of nutrient removal (N and P) of Synechococcus elongatus in novel core-shell capsules

Castro-Ceseña, Ana B.; Sánchez‐Saavedra, M. del Pilar; Ruíz-Güereca, Duahmet A.

doi:10.1007/s10811-015-0771-6

Cited by 13 publications

(12 citation statements)

References 27 publications

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“…B). This result is in agreement with literature studies, where higher photosynthetic activity at lower calcium concentration is observed due to greater nutrient diffusion enabled by a lesser degree of calcium‐alginate cross‐linking, causing a more flexible immobilization matrix (Castro‐Ceseña et al, ; Simpson et al, ). In subsequent harvests, biomass yields were remarkably uniform, with variances among 2‐ and 3‐day periods of 0.02−0.08 g DW m −2 , which is explained by delayed effects of calcium dilution on hydrogel flexibility and growth.…”

Section: Resultssupporting

confidence: 93%

“…This alginate composition enabled rigid hydrogels due to the high, ∼50% consecutive guluronic residue content. These hydrogel formulations are similar to the optimal formulation reported of submerged 2% calcium‐alginate hydrogel cultures, of Chlorella vulgaris for growth and retention (0.3:1 and 180 mM, respectively) (Lam and Lee, ) and S. elongatus for photosynthetic activity (90−180 mM) (Castro‐Ceseña et al, ). Hydrogel cultures were of 60‐mm diameter with thicknesses of 1.0, 4.0, or 4.6 mm.…”

Section: Methodssupporting

confidence: 81%

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Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

Pierobon

Riordon

Nguyen

et al. 2017

Biotech & Bioengineering

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High-density biomass production is currently only realized in biofilm-based photobioreactors. Harvest yields of whole biofilms are self-limited by daughter-upon-parent cell growth that hinders light and leads to respiratory biomass losses. In this work, we demonstrate a sustainable multi-harvest approach for prolonged generation of high-density biomass. Calcium-alginate hydrogel cultures loaded with Synechococcus elongatus PCC 7942 achieved production densities comparable to that of biofilms (10 cells/mL) and optimal total productivity in harvest periods of 2 or 3 days that allowed high-density surface growth without self-limiting cell buildup or surface death. Cross-linking calcium concentration had a strong influence on surface growth and harvest yields, especially in the first harvests. Subsequent harvests achieved more uniform biomass yields and distributions, unaffected by bulk respiration or light penetration. Collectively, these results demonstrate the feasibility of sustained, high-density biomass production by periodic harvesting within microalgal hydrogel cultures. Biotechnol. Bioeng. 2017;114: 2023-2031. © 2017 Wiley Periodicals, Inc.

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Section: Resultssupporting

confidence: 93%

Section: Methodssupporting

confidence: 81%

Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

Pierobon

Riordon

Nguyen

et al. 2017

Biotech & Bioengineering

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“…The use of refrigerated microalgae is a viable approach toward maintaining small cultures when there are bottlenecks in the production of microalgae. Further, S. elongatus can be used as a biofilter for "green water culture" due to its high capacity for removing nutrients, especially nitrogen and phosphorous (Aguilar-May & Sánchez-Saavedra, 2009;Castro-Ceseña et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…For example, Synechococcus elongatus was isolated from a shrimp farm in the coastal lagoon of Nayarit, México (21°32'N, 105°17'W) (AguilarMay, 2002). This cyanobacterium has been considered to have tremendous potential for aquaculture use due to its advantageous properties, such as its high tolerance to variations in light and temperature (Aguilar-May, 2002;Castillo-Barrera et al, 2002), biochemical composition (Campa-Ávila, 2002), ability to remove nutrients (Aguilar-May & Sánchez-Saavedra, 2009;Castro-Ceseña et al, 2015), and high food quality for the rotifer Branchionus plicatilis (Campa-Ávila, 2002). The diatom Chaetoceros muelleri is widely used as food for mollusks and is frequently used to feed Artemia in several commercial hatcheries (Gómez-Gil et al, 2002;López-Elías et al, 2003, 2005MeullerFeuga, 2004).…”

Section: Introductionmentioning

confidence: 99%

Potential of refrigerated marine cyanobacterium Synechococcus elongatus used as food for Artemia franciscana

Sánchez‐Saavedra

Paniagua-Chávez

2017

lajar

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ABSTRACT. The aim of this study was to evaluate the performance of the cyanobacterium Synechococcus elongatus after refrigerated storage (4°C) and examine its potential as food for Artemia franciscana. Non-axenic semicontinuous cultures of S. elongatus were maintained at 4°C for 8 weeks. In our bioassay, we fed A. franciscana with refrigerated S. elongatus and fresh cultures of the diatom Chaetoceros muelleri as a control. The S. elongatus cultures could be refrigerated for up to 8 weeks without loss of its viability or alteration in its growth rate, cell size, and proximate composition. Fresh and eight week-refrigerated cultures of S. elongatus were similar with regard to fatty acid profiles. Significant differences in fatty acid profiles were found between refrigerated S. elongatus and fresh C. muelleri cultures. Vibrio bacteria were not detected in any of the S. elongatus cultures (fresh or refrigerated) or fresh cultures C. muelleri. This work demonstrated that refrigerated S. elongatus can be used to feed A. franciscana, maintaining similar growth rates and proximate composition compared with fresh C. muelleri cultures as feed. Thus, S. elongatus has potential aquaculture use as feed for A. franciscana.

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“…Several studies that have compared alginate‐immobilized with suspended algae showed that immobilization increased, reduced, or had no effect on algal growth and nutrient removal . These comparisons, which were based on cultivation in synthetic media, may also not translate well to real wastewaters, with immobilization of Chlorella vulgaris found to increase ammonium (NH 4 + ) removal and reduce phosphate (PO 4 3− ) removal from real wastewater but have no impact when treating synthetic media .…”

Section: Introductionmentioning

confidence: 99%

Nutrient removal by alginate‐immobilized Chlorella vulgaris: response to different wastewater matrices

Kube

Spedding

Gao

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND: Immobilized algae are a promising tool to treat wastewater within a short time (<12 h) and simplify biomass harvesting compared with suspended algae. The potential of alginate-entrapped Chlorella vulgaris to bioremediate secondary (with and without nutrient supplementation), primary and two lagoon municipal wastewaters was investigated. The capability of the system to adapt to these wastewaters was analysed by determining biotic and abiotic nutrient removal, and further evaluated by comparison with suspended cells.RESULTS: The algal nitrogen (N) content (4.6-7.8 wt%) was closely related to the wastewater ammonium concentration (R 2 = 0.97). The algal cells did not adapt N uptake as effectively to wastewater nitrate concentration because both abiotic N and phosphorus (P) removal increased. The algal P content (1.2-3.2 wt%) varied in response to the wastewater P and was inversely related to the initial cellular P content. The algae thus adapted nutrient uptake to the wastewater N:P level and ratio when ammonium predominated. Biomass production (35-73 mg L -1 d -1 ) increased with dissolved organic and inorganic carbon with little impact from other wastewater characteristics. Immobilization did not affect N and P uptake per cell compared with suspended algae. CONCLUSION: Decoupling biotic and abiotic removal showed adaptation to the N:P of the wastewater and luxury P uptake had significant impact during treatment of different wastewater matrices, these traits were not affected by immobilization. Immobilization enables increased N and P removal rates compared with suspended algal systems as nutrient uptake per cell is not affected and higher concentrations of algal biomass within the reactor are facilitated.To meet the desired feed nutrient levels 184 mg L −1 NaNO 3 was added to SE1 + NO3, 115 mg L −1 of NH 4 Cl was added to SE1 + NH4, 268 mg L −1 of NaNO 3 to SE2 + NO3, and 156 mg L −1 of NH 4 Cl to SE2 + NH4.Immobilized algae adapt nutrient removal to different wastewaters www.soci.org

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Optimization of entrapment efficiency and evaluation of nutrient removal (N and P) of Synechococcus elongatus in novel core-shell capsules

Cited by 13 publications

References 27 publications

Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high‐density production

Potential of refrigerated marine cyanobacterium Synechococcus elongatus used as food for Artemia franciscana

Nutrient removal by alginate‐immobilized Chlorella vulgaris: response to different wastewater matrices

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