Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties

Huang, Shuigen; Deng, Guanjun; Yang, Ying; Wu, Zhengyan; Wu, Lifang

doi:10.1590/s1516-89132015050256

Cited by 14 publications

(6 citation statements)

References 29 publications

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“…Cellulolytic activity of marine actinomycetes is commonly observed [48][49][50][51]. In our study, strains that presented stronger cellulolytic activity belonged to the genera Streptomyces, Kocuria, Arthrobacter, Nesterenkonia, Faviflexus, and Brachybacterium, which was in agreement with other studies [52][53][54][55], with the exception of Flaviflexus. To our knowledge, this is the first report showing cellulolytic activity for a Flaviflexus strain.…”

Section: Discussionsupporting

confidence: 92%

Enzyme Bioprospection of Marine-Derived Actinobacteria from the Chilean Coast and New Insight in the Mechanism of Keratin Degradation in Streptomyces sp. G11C

et al. 2020

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Marine actinobacteria are viewed as a promising source of enzymes with potential technological applications. They contribute to the turnover of complex biopolymers, such as pectin, lignocellulose, chitin, and keratin, being able to secrete a wide variety of extracellular enzymes. Among these, keratinases are a valuable alternative for recycling keratin-rich waste, which is generated in large quantities by the poultry industry. In this work, we explored the biocatalytic potential of 75 marine-derived actinobacterial strains, focusing mainly on the search for keratinases. A major part of the strains secreted industrially important enzymes, such as proteases, lipases, cellulases, amylases, and keratinases. Among these, we identified two streptomycete strains that presented great potential for recycling keratin wastes—Streptomyces sp. CHA1 and Streptomyces sp. G11C. Substrate concentration, incubation temperature, and, to a lesser extent, inoculum size were found to be important parameters that influenced the production of keratinolytic enzymes in both strains. In addition, proteomic analysis of culture broths from Streptomyces sp. G11C on turkey feathers showed a high abundance and diversity of peptidases, belonging mainly to the serine and metallo-superfamilies. Two proteases from families S08 and M06 were highly expressed. These results contributed to elucidate the mechanism of keratin degradation mediated by streptomycetes.

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Section: Discussionsupporting

confidence: 92%

Enzyme Bioprospection of Marine-Derived Actinobacteria from the Chilean Coast and New Insight in the Mechanism of Keratin Degradation in Streptomyces sp. G11C

et al. 2020

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“…C. freundii , A. woluwensis , and B. licheniformis were isolated from the gut of root-feeding larvae Holotrichia parallela and found to possess cellulolytic activity in our previous studies [24,25]. For constructing bacterial mixture, C. freundii , A. woluwensis , and B. licheniformis grew in Luria-Bertani (LB) at 30 °C for 20–24 h in a rotary shaker incubator (180 rpm), respectively.…”

Section: Methodsmentioning

confidence: 99%

High Performance Bacteria Anchored by Nanoclay to Boost Straw Degradation

Tang

Chen

et al. 2019

Materials

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Generally, crop straw degrades slowly in soil, which is unfavorable for tillage and next crop growth. Thus, it is important to develop a promising technology to boost degradation of straw. Herein, a nanobiosystem has been developed by loading bacterial mixture in nanostructured attapulgite (ATP) and using it as a straw returning agent (SRA). Therein, ATP could effectively anchor bacteria to the surface of straw and greatly facilitate the adhesion and growth of bacteria. Consequently, this technology could effectively accelerate the degradation and transformation of straw into nutrients, including nitrogen (N), phosphorus (P), potassium (K), and organic matters (OM). Pot and field tests indicated that SRA displayed significant positive effects on the growth of the next crop. Importantly, SRA could effectively decrease greenhouse gas emissions from farmland, which is beneficial for the environment. Therefore, this work provides a facile and promising method to facilitate the degradation of straw, which might have a potential application value.

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“…In general, microorganisms are the main source of cellulases, and are naturally specialized in degrading and recycling different types of plant biomass. As such, fungi are of the great importance for the industrial production of cellulases, due to their ability to produce a large amount and variety of these enzymes, which are secreted in the fungal growth substrate and improve the extraction and purification of these molecules (Huang et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Endoglucanase production by trichoderma reesei cultivated in solid state fermentation using lignocellulosic waste / Utilização de resíduos lignocelulósicos para a produção de endoglucanases por trichoderma reesei cultivado sob fermentação em estado sólido

Pessoa¹,

Júnior²,

Chevreuil³

et al. 2021

BJDV

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Trichoderma reesei is a fungus that has been widely explored for its potential as cellulolytic enzyme producer and has diverse industrial applications. However, obtaining the enzymes is still considered a costly and, sometimes, inefficient process. This study aimed to produce endoglucanases by cultivating T. reesei (CCT-2768) in solid state fermentation, using cashew apple bagasse (CAB), sugarcane bagasse (SCB) and green coconut fiber (GCF) residues as substrates. The influence of moisture and pH on enzyme production was evaluated using a factorial design. T. reesei showed viability for producing endoglucanases in all of the three lignocellulosic residues tested, with maximum activity (2.29 ± 0.01 U/g) observed when cultivated in the SCB substrate and using moisture of 60% and pH 5.5. Thus, use of lignocellulosic residues proves to be a viable alternative for producing endoglucanases by cultivation of Trichoderma reesei, which contributes to the recycling of waste and the reduction of environmental impacts.

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Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties

Abstract: In this study, a potential novel cellulolytic bacterium

Cited by 14 publications

References 29 publications

Enzyme Bioprospection of Marine-Derived Actinobacteria from the Chilean Coast and New Insight in the Mechanism of Keratin Degradation in Streptomyces sp. G11C

Enzyme Bioprospection of Marine-Derived Actinobacteria from the Chilean Coast and New Insight in the Mechanism of Keratin Degradation in Streptomyces sp. G11C

High Performance Bacteria Anchored by Nanoclay to Boost Straw Degradation

Endoglucanase production by trichoderma reesei cultivated in solid state fermentation using lignocellulosic waste / Utilização de resíduos lignocelulósicos para a produção de endoglucanases por trichoderma reesei cultivado sob fermentação em estado sólido

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