Endoglucanase production by trichoderma reesei cultivated in solid state fermentation using lignocellulosic waste 	/ Utilização de resíduos lignocelulósicos para a produção de endoglucanases por trichoderma reesei cultivado sob fermentação em estado sólido

Pessoa, Vítor Alves; Júnior, Sérgio Dantas de Oliveira; Chevreuil, Larissa Ramos; Aguiar, Lorena Vieira Bentolila de; Silva, Jefte Farias da; Gouvêa, Paula Romenya dos Santos; Sales-Campos, Ceci; Santos, Everaldo Silvino dos

doi:10.34117/bjdv7n11-264

Cited by 2 publications

(3 citation statements)

References 18 publications

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“…It was indicated that a 2.73-fold increase production of endoglucanase was observed compared to the produced CMCase, under an unoptimized conditions (153.467 ± 6.897 U/g). The reported SSF parameters using Trichoderma species were initial moisture content of 40 -60% [18], pH > 5.5 Mandel's medium [27], and incubation temperature of 30 -32 °C [7,26], which agreed well with the with the current study.…”

Section: Resultssupporting

confidence: 89%

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Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Teo¹,

Wahab

2023

J.Trop.Life.Science

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The oil palm biomass produced by plantations and mills in Malaysia is the largest contributor to the nation’s agro-waste, with the oil palm leaves (OPL) topping the list. Nevertheless, the surplus of OPL might have applicability as the substrate for cultivating lignocellulolytic bio-degraders. Ipso facto, this study employed raw OPL as the carbon source for cultivating Trichoderma harzianum under solid-state fermentation (SSF). Optimization of the SSF process using the Taguchi orthogonal design to produce endoglucanase (CMCase) successfully established the optimal fermentation conditions as the following: 7.00 × 108 spore/g inoculum size, 50% moisture content, pH 12 Mandel’s medium, with 3-day incubation at 40°C. The crude enzyme cocktail exhibited the corresponding maximum activity of 417.49 U/g CMCase. The enzymatic activities were significantly affected by factors, moisture content, inoculum size, and initial pH (p-value < 0.05). In short, the high extracellular CMCase activity of the T. harzianum crude enzyme cocktail may prove valuable in accelerating the saccharification of cellulose for biofuel-and nanocellulose production.

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Section: Resultssupporting

confidence: 89%

“…was at pH > 5.5. This value supports most of the industrial applications under an alkaline situation [26,27]. Interestingly, mutual additive effect between the inoculum size (A) and the initial pH (C) was observed by the red colour, which represents the maximum pH and inoculum size (Figure 2ef).…”

Section: Resultssupporting

confidence: 77%

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Teo¹,

Wahab

2023

J.Trop.Life.Science

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“…Generally, SSF studies use the moisture content of the medium between 50 and 70%, depending on the specific capacity of the material to retain water, which is represented by the water activity [47][48][49][50]. Pessoa et al (2021) [51] evaluated the effect of moisture and pH on the production of endoglucanases through SSF using T. reesei CCT-2768 applied to three different lignocellulosic substrates (green coconut fiber, sugarcane bagasse, and cashew apple bagasse). It was observed that the best moisture varied among the three substrates, being 80% (1.55 U/g), 60% (2.29 U/g), and 70% (1.46 U/g), respectively.…”

Section: Runmentioning

confidence: 99%

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Campos,

Asevedo,

Souza Filho

et al. 2024

Biomass

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The industrial processing of coconut to produce valuable foods, such as water and milk, generates large volumes of waste, especially the fruit shell. Despite this, material can be used in bioprocess applications, e.g., the production of enzymes, its recalcitrance hinders the cultivation of microorganisms, and low productivity is usually achieved. In this study, the production of cellulolytic enzymes through solid-state fermentation (SSF) and their extraction was investigated using the green coconut fiber pretreated by steam explosion, followed by alkali. The fungus Trichoderma reesei CCT-2768 was cultivated, using an experimental design, to study the effect of the water activity and the amount of biomass in the reactor. The combination of the pretreatment strategies yielded more porous biomass, with less hemicellulose (5.38%, compared to 10.15% of the raw biomass) and more cellulose (47.77% and 33.96% in the pretreated and raw biomasses, respectively). The water activity significantly affected the production of cellulases, with maximum activity yielded at the highest investigated value (0.995). Lastly, the extraction of the enzymes from the cultivation medium was studied, and a 9 g/L NaCl solution recovered the highest CMCase and FPase activities (5.19 and 1.19 U/g, respectively). This study provides an important contribution to the valorization of the coconut residue through (i) the application of the steam explosion technology to optimize the production of cellulases using the SSF technology and (ii) their extraction using different solvents.

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Endoglucanase production by trichoderma reesei cultivated in solid state fermentation using lignocellulosic waste / Utilização de resíduos lignocelulósicos para a produção de endoglucanases por trichoderma reesei cultivado sob fermentação em estado sólido

Cited by 2 publications

References 18 publications

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

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