Optimization of Electroporation Conditions for Arthrobacter simplex

Luo, Jianmei; Xue, Haijie; Zhao, Shengnan; Liu, Xiangsheng; Chen, Xiangrong; Li, Jiajia; Shen, Yanbing

doi:10.1007/978-981-10-4801-2_40

Cited by 1 publication

(1 citation statement)

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“…The most common biological application for electropermeabilization is the introduction of alien materials into cells wherein the membranes are usually impermeable. In the case of gene transfer, for example, even larger molecules (such as DNA fragments), which are naturally not able to pass the cell membrane, can be introduced [18,19]. In order to treat a large number of cells simultaneously (e.g., within a cell suspension), electroporation cuvettes are normally used, consisting of two capacitor plates.…”

Section: Introductionmentioning

confidence: 99%

Printed Flexible Microelectrode for Application of Nanosecond Pulsed Electric Fields on Cells

et al. 2019

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Medical treatment is increasingly benefiting from biomedical microsystems, especially the trending telemedical application. A promising modality for tumor therapy showed the application of nanosecond pulsed electric fields (nsPEF) on cells to achieve nanoporation, cell death, and other cell reactions. A key technology for this method is the generation of pulsed fields in the nanosecond range with high-field strengths in the range of several kilovolts per centimeter. For further biomedical applications, state-of-the-art setups need to decrease in size and improve their capability of integration into microsystems. Due to demanding electronic requirements, i.e., using high voltages and fast pulses, miniaturization and low-cost fabrication of the electrode is first considered. This paper proposes a proof-of-concept for a miniaturized printed flexible electrode that can apply nsPEF on adherent fibroblast cells. The interdigital gold electrode was printed on polyimide with line-width of about 10 µm using an electrohydrodynamic inkjet printer. Furthermore, an electrical circuit was developed to generate both electrical pulses in the nano-second range and voltages up to 180 V. The electrode was integrated into an experimental setup for in-vitro application to human fibroblasts. Field strengths up to 100 kV/cm with 45 ns pulse duration were applied, depending on the degree of cell confluence. The cells show contraction, detachment from the electrode, and lethal reactions after the nsPEF treatment. Furthermore, this printed miniaturized electrode was found to be suitable for subsequent microsystem integration and further cell experiments to optimize pulse parameters for control of cell reaction and behavior.

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Section: Introductionmentioning

confidence: 99%