Optimization of Dietary Restriction Protocols in Drosophila

Bass, Timothy M.; Grandison, Richard C.; Wong, Richard; Martı́nez, Pedro; Partridge, Linda; Piper, Matthew D.W.

doi:10.1093/gerona/62.10.1071

Cited by 281 publications

(365 citation statements)

References 42 publications

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“…1a-d). The base and DR diets used here are routinely employed in aging intervention and DR studies in D. melanogaster (Bross et al 2005;Bass et al 2007a;Zou et al 2007). Our findings suggest that supplementation of resveratrol at up to 200 μM is not sufficient to promote longevity of flies under standard or restricted dietary conditions.…”

Section: Resultsmentioning

confidence: 99%

The effect of resveratrol on lifespan depends on both gender and dietary nutrient composition in Drosophila melanogaster

Wang

Wheeler

Alberico

et al. 2011

AGE

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Resveratrol, a polyphenolic compound, has been shown to extend lifespan in different organisms. Emerging evidence suggests that the prolongevity effect of resveratrol depends on dietary composition. However, the mechanisms underlying the interaction of resveratrol and dietary nutrients in modulating lifespan remain elusive. Here, we investigated the effect of resveratrol on lifespan of Drosophila melanogaster fed diets differing in the concentrations of sugar, yeast extract, and palmitic acid representing carbohydrate, protein, and fat, respectively. Resveratrol at up to 200 μM in diets did not affect lifespan of wild-type female flies fed a standard, restricted or high sugar-low protein diet, but extended lifespan of females fed a low sugar-high protein diet. Resveratrol at 400 μM extended lifespan of females fed a high-fat diet. Lifespan extension by resveratrol was associated with downregulation of genes in aging-related pathways, including antioxidant peroxiredoxins, insulin-like peptides involved in insulin-like signaling and several downstream genes in Jun-kinase signaling involved in oxidative stress response. Furthermore, resveratrol increased lifespan of superoxide dismutase 1 (sod1) knockdown mutant females fed a standard or high-fat diet. No lifespan extension by resveratrol was observed in wild-type and sod1 knockdown males under the culture conditions in this study. Our results suggest that the gender-specific prolongevity effect of resveratrol is influenced by dietary composition and resveratrol promotes the survival of flies by modulating genetic pathways that can reduce cellular damage. This study reveals the context-dependent effect of resveratrol on lifespan and suggests the importance of dietary nutrients in implementation of effective aging interventions using dietary supplements.

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Section: Resultsmentioning

confidence: 99%

The effect of resveratrol on lifespan depends on both gender and dietary nutrient composition in Drosophila melanogaster

Wang

Wheeler

Alberico

et al. 2011

AGE

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“…The treatment effects included in this study spans many of the variables that currently are thought to have a strong implication for lifespan, and the full-factorial design employed makes it possible to thoroughly analyze their effect as well as potential interactions between them. It is, however, important to note that longevity data are very sensitive even to small changes in experimental procedures, e.g., the type and manufacturer of yeast used in the diet (Bass et al 2007). …”

Section: Experimental Designmentioning

confidence: 99%

Interactive effects of sex, social environment, dietary restriction, and methionine on survival and reproduction in fruit flies

Zajitschek

Friberg

et al. 2012

AGE

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For the evolution of life histories, the trade-off between survival and reproduction is fundamental. Because sexes optimize fitness in different ways, this trade-off is expected to be resolved differently by males and females. Consequently, the sexes are predicted to respond differently to changes in resource availability. In fruit flies, research on dietary restriction has focused largely on females maintained in the absence of males, thereby neglecting sexual interactions that affect reproductive behavior of both sexes under more natural conditions. Here, we tested for the interactive effects of diet (40, 60, 100, and 300 % of standard yeast concentrations) and social environment (separate-sex vs. mixed-sex groups) on male and female Drosophila melanogaster life histories. Additionally, we evaluated the essential amino acid methionine as an agent that can uncouple the survival-reproduction trade-off. We show sex differences in the effect of social environment on survival patterns, but not on reproductive fitness. In females, yeast had a positive effect on reproduction and a negative effect on survival. In males, yeast had a negative effect on reproduction and the effect on survival depended on the social environment. Methionine reduced survival, but had no effect on reproduction. Our findings highlight the need to include both sexes and to vary social environments in research programs aimed at lifespan extension and call for further evaluation of the fecundityrestoring effect of methionine.

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“…The Drosophila strain w 1118 was used in all control crosses and as the background for the generation of transgenic lines. All stocks were maintained and all the experiments were conducted at 25 °C on a 12‐hr:12‐hr light:dark cycle at constant humidity using standard sugar/yeast/agar (SYA) media (Bass et al ., 2007). For all the experiments, flies were reared at standard larval density, and enclosing adults were collected over a 12‐h period.…”

Section: Methodsmentioning

confidence: 99%

β‐Guanidinopropionic acid extends the lifespan of Drosophila melanogaster via an AMP‐activated protein kinase‐dependent increase in autophagy

Yang

Long

et al. 2015

Aging Cell

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SummaryPrevious studies have demonstrated that AMP‐activated protein kinase (AMPK) controls autophagy through the mammalian target of rapamycin (mTOR) and Unc‐51 like kinase 1 (ULK1/Atg1) signaling, which augments the quality of cellular housekeeping, and that β‐guanidinopropionic acid (β‐GPA), a creatine analog, leads to a chronic activation of AMPK. However, the relationship between β‐GPA and aging remains elusive. In this study, we hypothesized that feeding β‐GPA to adult Drosophila produces the lifespan extension via activation of AMPK‐dependent autophagy. It was found that dietary administration of β‐GPA at a concentration higher than 900 mm induced a significant extension of the lifespan of Drosophila melanogaster in repeated experiments. Furthermore, we found that Atg8 protein, the homolog of microtubule‐associated protein 1A/1B‐light chain 3 (LC3) and a biomarker of autophagy in Drosophila, was significantly upregulated by β‐GPA treatment, indicating that autophagic activity plays a role in the effect of β‐GPA. On the other hand, when the expression of Atg5 protein, an essential protein for autophagy, was reduced by RNA interference (RNAi), the effect of β‐GPA on lifespan extension was abolished. Moreover, we found that AMPK was also involved in this process. β‐GPA treatment significantly elevated the expression of phospho‐T172‐AMPK levels, while inhibition of AMPK by either AMPK‐RNAi or compound C significantly attenuated the expression of autophagy‐related proteins and lifespan extension in Drosophila. Taken together, our results suggest that β‐GPA can induce an extension of the lifespan of Drosophila via AMPK‐Atg1‐autophagy signaling pathway.

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Optimization of Dietary Restriction Protocols in Drosophila

Cited by 281 publications

References 42 publications

The effect of resveratrol on lifespan depends on both gender and dietary nutrient composition in Drosophila melanogaster

The effect of resveratrol on lifespan depends on both gender and dietary nutrient composition in Drosophila melanogaster

Interactive effects of sex, social environment, dietary restriction, and methionine on survival and reproduction in fruit flies

β‐Guanidinopropionic acid extends the lifespan of Drosophila melanogaster via an AMP‐activated protein kinase‐dependent increase in autophagy

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