Optimization of diagnosis of SARS-CoV-2 infection using polymerase chain reaction in a large multi-specialty hospital

Жданов, К. В.; Козлов, К. В.; Bulankov, Yu. I.; Захаренко, С. М.; Улюкин, Игорь Михайлович; Zagorodnikov, G. G.; Григорьев, С. Г.; Orlova, E. S.; Shuklina, A. A.; Ivanov, A. M.; Maltsev, Oleg V.; Сукачев, В. С.; Kasyanenko, Kristina V.; Grinchenko, N. E.

doi:10.17816/brmma50036

Cited by 9 publications

(1 citation statement)

References 2 publications

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“…Почти у всех детей к этому времени РНК SARS-Cov-2 в назофарингеальных мазках не определялась. Возможно, это связано с большим количеством тяжелых форм в виде пневмоний у взрослых, при которых, согласно литературным данным, выделение РНК SARS-Cov-2 происходит дольше [12].…”

Section: результаты и их обсуждениеunclassified

New coronavirus infection (COVID-19) in children: clinical, laboratory and diagnostic aspects

2021

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The study of a new coronavirus infection (COVID-19) in children is relevant and of interest for practical health care.Purpose: to identify the clinical and laboratory features of COVID-19 in the context of the six-month dynamics of the pandemic in children of the Orenburg region and to formulate recommendations for optimizing the diagnosis of this disease.Materials and methods. A retrospective analysis of 2661 results of detecting SARS-CoV-2 RNA (including 170 children) was carried out on the basis of the microbiological laboratory of the Orenburg State Medical University. Age and sex, structure of preliminary diagnoses, main clinical manifestations, frequency of testing and dynamics of the PCR threshold cycle in September 2020 and January 2021 were assessed.Results. The proportion of children examined in the Orenburg region from September 2020 to January 2021 increased from 4.6 ± 0.6% to 8.1 ± 0.7%. At the same time, the number of PCR + results also significantly increased from 5.0 ± 2.8% to 8.2 ± 2.6% (χ2 = 54.81; p = 0). The average age of children was 9—10 years old. The main reason for referral was pre-hospitalization screening not related to COVID-19. The proportion of PCR + results in this cohort was significantly lower in children than in adults. Among the clinically manifested forms in adults, the leading diagnosis was «pneumonia», and in children «ARVI». In childhood, a predominantly asymptomatic course of the disease was recorded. In children, unlike adults, PCR Ct values did not correlate with the severity and duration of the clinical manifestations of COVID-19.Conclusion. Due to the low diagnostic value of symptoms in children, it is more expedient to focus on epidemiological data and data from the results of laboratory examination methods. When interpreting the results of PCR diagnostics of COVID-19 for prognostic purposes, it is important to take into account the patient's age.

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Section: результаты и их обсуждениеunclassified

New coronavirus infection (COVID-19) in children: clinical, laboratory and diagnostic aspects

2021

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Experience of Military Medicine in the Fight against the New Coronavirus Infection

Kryukov

Trishkin²,

Салухов³

et al. 2022

Her. Russ. Acad. Sci.

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The results achieved by the medical service of the Armed Forces of the Russian Federation to overcome the new coronavirus infection are presented. The high efficiency of the established unified system of biological safety and strict ranking of the priorities of anti-epidemic measures is shown. The experience of organizing traveling medical and nursing teams, as well as temporary military medical units on the territory of Russia, as well as in foreign countries, is demonstrated. Among the priority scientific results of military doctors, especially noteworthy is the study of the world’s first COVID-19 vaccine Sputnik V, its immunogenicity, the effectiveness of its use in previously ill patients and revaccination, as well as the use of immune plasma from those who have been ill and vaccinated. By the examples of organized military groups and the general population, the features of the formation of herd immunity have been studied. Military doctors were the first in the country to show the effectiveness of hormone therapy in the treatment of coronavirus infection and to study its effects. They carried out ultrastructural studies of the life cycle of the virus. It is shown that the system of comprehensive measures implemented by military medicine determined a lower incidence of new coronavirus infection among the personnel of the Ministry of Defense of Russia and a lower mortality among the military.

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Assessment of post-vaccination collective immunity against new coronavirus infection (COVID-19) among servicemen of the Armed Forces of the Russian Federation

Азаров

Ovchinnikov

Kuzin

et al. 2022

Bulletin of the Russian Military Medical Academy

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The recent vaccination campaign targeting the new coronavirus infection (COVID-19) carried out in the Armed Forces of the Russian Federation, on the background of the current unstable global pandemic situation, makes it necessary to study post-vaccination population immunity to the SARS-CoV-2 virus and thus identify key features of immunity in organized military collectives. In the future, this will make it possible to objectively assess the risks of a worsening pandemic situation, effectively adjust the ongoing sanitary and anti-epidemic measures aimed at preserving and strengthening the health of military personnel, as one of the main conditions for maintaining the combat readiness of the Armed Forces of the Russian Federation. During a study conducted on epidemic indications, it was found that vaccination with Gam-Covid-Vac contributes to the formation of collective immunity with 95% effectiveness. A gender-based analysis of the immune response showed that the proportion of persons who lack class G immunoglobulins to SARS-CoV-2 among females is twice than that among men (9.3% and 4.7%, respectively). Seroprevalence indicators, classified by blood group, range from 94.4% (AB (IV) Rh) to 97.4% (A (II) Rh). There were no significant differences in seroprevalence between groups of people with different blood groups; however, the highest value of seroprevalence was seen among military personnel with blood group A (II) Rh. In this context, it is advisable to continue monitoring the formation of immunity in individuals with various blood groups. The results obtained made it possible to form a primary medical and social portrait of a serviceman with the most adequate immune response to the introduction of the Gam-Covid-Vac vaccine (a man under the age of 20 with blood type A (II) Rh) and to draw a conclusion about the high effectiveness of vaccination in military units (formations) staffed by conscripts and military educational organizations.

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Optimization of diagnosis of SARS-CoV-2 infection using polymerase chain reaction in a large multi-specialty hospital

Cited by 9 publications

References 2 publications

New coronavirus infection (COVID-19) in children: clinical, laboratory and diagnostic aspects

New coronavirus infection (COVID-19) in children: clinical, laboratory and diagnostic aspects

Experience of Military Medicine in the Fight against the New Coronavirus Infection

Assessment of post-vaccination collective immunity against new coronavirus infection (COVID-19) among servicemen of the Armed Forces of the Russian Federation

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