Optimization of culture media to enhance the ability of local Bacillus thuringiensis var. tenebrionis

Saberi, Fatemeh; Marzban, Rasoul; Ardjmand, Mehdi; Shariati, Farshid Pajoum; Tavakoli, Omid

doi:10.1016/j.jssas.2020.08.004

Cited by 12 publications

(16 citation statements)

References 18 publications

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“…Biopesticides have several merits over conventional chemical pesticides. They are environmentally friendly, target specific, and not deleterious to nontarget organisms and hence potent enough to replace synthetic pesticides for pest management [46]. Table 2 provides an overview of the disadvantages of using conventional chemical pesticides instead of biopesticides.…”

Section: Merits Of Biopesticides Over Chemical Pesticidesmentioning

confidence: 99%

An Overview of Some Biopesticides and Their Importance in Plant Protection for Commercial Acceptance

Kumar

Ramlal

Mallick

et al. 2021

Plants

219

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Biopesticides are natural, biologically occurring compounds that are used to control various agricultural pests infesting plants in forests, gardens, farmlands, etc. There are different types of biopesticides that have been developed from various sources. This paper underscores the utility of biocontrol agents composed of microorganisms including bacteria, cyanobacteria, and microalgae, plant-based compounds, and recently applied RNAi-based technology. These techniques are described and suggestions are made for their application in modern agricultural practices for managing crop yield losses due to pest infestation. Biopesticides have several advantages over their chemical counterparts and are expected to occupy a large share of the market in the coming period.

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Section: Merits Of Biopesticides Over Chemical Pesticidesmentioning

confidence: 99%

An Overview of Some Biopesticides and Their Importance in Plant Protection for Commercial Acceptance

Kumar

Ramlal

Mallick

et al. 2021

Plants

219

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“…tenebrionis-BN1 using molasses from sugar beet and sugar cane as a carbon source while using corn steep liquor as a nitrogen source. The maximum CFU of 10 7 spore/ml was achieved with the amount of carbon source above 11% and nitrogen source less than 2% [6]. For Bt, the media that support the growth and sporulation consists of: glucose, 5 g/L; glycerol, 1 g/L; sodium acetate, 0 g/L; (NH4)2SO4, 1 g/L; corn steep liquor, 2 mL/L; yeast extract, 1 g/L; hydrolyzed casein, 1 g/L; CaCl2, 20 mg/L; MgSO4, 50 mg/L; MnSO4 100 mg/L provided the growth 6.4 x 10 -7 cell/mL and 99% of sporulation [10].…”

Section: Introductionmentioning

confidence: 95%

“…subspecies israelensis in conventional medium Soyabean Casein Digest medium TSB (HIMEDIA) was prepared for 300 mL per batch in triplicate. The Bti was subcultured in modified TSB [6] overnight as inoculum. Three percent (v/v) of Bti inoculum was added to the TSB and incubated at room temperature (approximately 21.5˚C) under shaking (100 rpm) and static conditions (Figure 1).…”

Section: Determination Of Kinetic Growth Of Bacillus Thuringiensismentioning

confidence: 99%

“…Bti was cultured overnight on the orbital shaker at 30˚C 100 rpm in the modified TSB [6] as inoculum culture.…”

Section: Culturing In Saberi Medium and Modified Saberi Mediamentioning

confidence: 99%

“…The function of the gut is disrupted, causing larval death from starvation [4] The Bti production for mosquito control using laboratory media is expensive. Previous work by Saberi (2020) [6] optimized the culture media for the production of B. thuringiensis var. tenebrionis-BN1 using molasses from sugar beet and sugar cane as a carbon source while using corn steep liquor as a nitrogen source.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of low-cost media for Bacillus thuringiensis subspecies israelensis

Mo-on

Panprivech²,

Kunathigan³

2022

E3S Web Conf.

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Bacillus thuringiensis subspecies israelensis (Bti) has been used as a mosquito control agent in agricultural field and residences. The production of Bti by conventional medium was expensive. The objective of this study was to investigate low-cost media for Bti’s production with a cost analysis of the medium formulated. Soybean Casein Digest Medium (TSB) was used as a conventional medium to compare with Saberi medium at room temperature (approximately 21.5˚C) on static and agitation (by obituary shaker at 100 rpm). Saberi medium was also modified to vary the percentage of nitrogen and carbon source to observe the growth rate and sporulation, compared to the Saberi medium. The growth of Bti in TSB with agitation for 24 hours yielded the highest growth rate. Sporulation of Bti in TSB with agitation at room temperature was 6 days which was faster than Bti in the Saberi medium. Modified Saberi nitrogen source and carbon source medium could not support the growth of Bti due to an imbalance in the percentage of nitrogen source and carbon source. Determination of sugar utilization showed sugar in the medium was utilized mostly in the exponential phase of Bti, from 3 hours to 6 hours. The price of TSB was 76.80 Thai baht per liter while Saberi medium’s cost was 20.43 Thai baht per liter. In this study, the Saberi medium may not be able to replace TSB in terms of growth rate and period of sporulation. Further study will be needed to optimize the media that use agro-industrial wastes as ingredients following the concept of circular economy and sustainability.

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Pesticides and Crop Protection

Nazeer,

Ahmad,

Verma

et al. 2023

Nanotechnology in the Life Sciences

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Optimization of culture media to enhance the ability of local Bacillus thuringiensis var. tenebrionis

Cited by 12 publications

References 18 publications

An Overview of Some Biopesticides and Their Importance in Plant Protection for Commercial Acceptance

An Overview of Some Biopesticides and Their Importance in Plant Protection for Commercial Acceptance

Investigation of low-cost media for Bacillus thuringiensis subspecies israelensis

Pesticides and Crop Protection

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