Optimization of culture media of pathogenicMycoplasma hyopneumoniaeby a response surface methodology

Abstract

Composition of culture medium for mass production of Mycoplasma hyopneumoniae was optimized using a response surface methodology (RSM). Initially, the influence of glucose, thallium acetate, fresh yeast extract, horse serum, and porcine serum on the production of mycoplasmal protein was assessed using a 'one factor at a time' technique. Next, factors with a significant effect, including fresh yeast extract, and horse and porcine sera, were selected for further optimization using a central composite design (CCD) of RSM. The experimental results were fitted into a second order polynomial model equation. Estimated optimal condition of the factors for maximum production of mycoplasmal protein (i.e., triple-fold increase from 0.8 mg/L produced by basal mycoplasma media to 2.5 mg/L) was 10.9% fresh yeast extract, 15% horse serum, and 31.5% porcine serum (v/v). For the optimized conditions, a 2.96 mg/L experimental result was observed, similar to the estimated optimal conditions result of the CCD.

The Mycoplasma spp. ‘Releasome’: A New Concept for a Long-Known Phenomenon

“…The authors pointed out that the high polypeptide concentrations in the growth media contributed by components such as serum dramatically jeopardizes the purification of proteins released by mycoplasmas, as is the case for exoproteins from eukaryotic cells that require serum for growth (Armengaud et al, 2012). Indeed, the 20% serum supplementation, usually used in mycoplasma medium, adds up to 12 g of proteins/L, which is dramatically more than the 2 mg of mycoplasma biomass/L reached by M. hyopneumoniae under optimized growth conditions (Hwang et al, 2010). A simple solution is to lower the relative concentrations of serum, as has been done to study the exoproteome of M. hyopneumoniae/M.…”

Thallium-induced DNA damage, genetic, and epigenetic alterations

“…Since its discovery, Tl has been used in industrial applications as rodenticide, cement, insecticides, alloys, electronic devices, special glass, and optical lenses, in the industries of photography, semiconductors, and superconductivity ( Taylor and McKillop, 1970 ). In addition, it has been used as a constituent of the growth media used for culture of some species of Mycoplasma ( Hwang et al, 2010 ; Puschner et al, 2012 ). It is well documented that some anthropogenic activities such as the pyrite mining, smelting and related steel-making industry are also important source for Tl and other toxic metalloids pollution in the environment with the consequences in increasing the associated health risks ( Wang et al, 2021 ).…”

Biofilm formation and determination of minimum biofilm eradication concentration of antibiotics in <i>Mycoplasma hyopneumoniae</i>

“…Despite the use of many antibiotics in pigs, more than 60% of the pig farms in Korea are infected with M. hyopneumoniae . We have been studying the pathogenesis of mycoplasmas with the objective of finding an effective treatment [ 5 , 11 , 12 , 19 ]. Despite a wealth of information on other species of mycoplasma and bacteria [ 4 , 13 , 14 , 15 , 16 ], biofilm formation and its effect on antimicrobials used for the treatment of enzootic pneumonia caused by M. hyopneumoniae in pigs is not studied.…”