Optimization of Culture Conditions for Maximal Lovastatin Production by Aspergillus terreus (KM017963) under Solid State Fermentation

Kamath, Praveen Vadakke; Dwarakanath, Bhargavi Santebennur; Chaudhary, Avinash; Janakiraman, Savitha

doi:10.1016/j.hjb.2015.11.001

Cited by 28 publications

(23 citation statements)

References 10 publications

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“…Moreover, most of the lovastatin yield was detected in cells extracted with EtOAc (CE) rather than in the broth extracts (BE). This result is not in accordance with the results of cultivation using submerged fermentation, in which most of the lovastatin was found in the broth extracts (Kamath et al 2015;Mulder et al 2015). The separation of mycelial film from broth is also easier than that of spherical pellets from broth leading to easier extraction of the lovastatin.…”

Section: Cultivation Systemcontrasting

confidence: 70%

“…Lovastatin is produced as a secondary metabolite of the polyketide pathway by various fungi such as Monascus spp., Beauveria bassiana, Aspergillus fumigates and Aspergillus terreus (Mukhtar et al 2014). Among the various lovastatin producing species, A. terreus is well known as the most productive (Marcin & Marta 2011;Samiee et al 2003) and can be grown under submerged fermentation (SmF) or solid state fermentation (SSF) (Kamath et al 2015;Mulder et al 2015). Grown under submerged cultivation in soluble starch and sodium glutamate as, respectively, the carbon and nitrogen sources, the lovastatin production from A. terreus LA414 was 523.9 mg/L after cultivation for 8 days at a temperature of 28°C (Jia et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

Suwannarat¹,

Iewkittayakorn²,

Sukpondma³

et al. 2019

JSM

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The effect of lovastatin on cholesterol synthesis is well known. Although lovastatin has been produced from several Aspergillus species and cultivation systems, the yield of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 in a static liquid system has not yet been determined. Therefore, the objective of this study was to optimize lovastatin production from this strain using a central composite design developed through response surface methodology (RSM). The inoculum was prepared from mycelium or spore cultured in static liquid and shaking systems with potato dextrose broth (PDB) as the medium. The best inoculum preparation and cultivation systems were selected to study the optimization of the four fermentation parameters of medium concentration (X-5X), temperature (25-37°C), culture medium to flask volume ratio (2.5-12.5) and time (7-35 days). The best inoculum preparation was the mycelium method and the best cultivation system was the static liquid system. In the optimization procedure, a yield of 1315.69 mg/L lovastatin was obtained at a PDB concentration of 5X, a temperature of 25°C, a medium to flask volume ratio of 1:2.5 and a time of 21 days. In a model employing the optimized parameters, temperature was found to have the most effect on lovastatin production, which will decrease if temperature increases above 25°C. Based on this study, A. sclerotiorum PSU-RSPG178 can be a useful strain for producing lovastatin and RSM as an optimization technique not only reduced the number of experiments but also resulted in an optimized lovastatin yield. ABSTRAK Kesan lovastatin pada sintesis kolesterol telah diketahui. Walaupun lovastatin dihasilkan daripada beberapa spesiesAspergillus dan sistem penanaman, hasil bagi lovastatin daripada Aspergillus sclerotiorum PSU-RSPG178 dalam sistem cecair statik masih belum ditentukan. Oleh itu, objektif kajian ini adalah untuk mengoptimumkan pengeluaran lovastatin daripada strain ini menggunakan reka bentuk komposit pusat yang dibangunkan melalui kaedah gerak balas permukaan (RSM). Inokulum disediakan daripada miselium atau spora dikultur dalam cecair statik dan sistem goncangan dengan kaldu kentang dektrosa (PDB) sebagai medium. Sistem penyediaan dan penanaman inokulum terbaik telah dipilih untuk kajian pengoptimuman empat parameter penapaian kepekatan sederhana (X-5X), suhu (25-37°C), kultur medium kepada nisbah isi padu kelalang (2.5-12.5) dan masa (7-35 hari). Persediaan inokulum terbaik adalah kaedah miselium dan sistem penanaman terbaik adalah sistem cecair statik. Dalam prosedur pengoptimuman, hasil lovastatin sebanyak 1315.69 mg/L telah diperoleh pada kepekatan PDB 5X, suhu 25°C, medium kepada nisbah isi padu kelalang 1:2.5 dan tempoh masa 21 hari. Model yang menggunakan parameter optimum, suhu didapati memberi kesan ketara pada pengeluaran lovastatin, yang akan berkurang jika suhu meningkat melebihi 25°C. Berdasarkan kajian ini, A. sclerotiorum PSU-RSPG178 boleh menjadi strain yang berguna untuk menghasilkan lovastatin dan RSM sebagai teknik pengoptimuman yang bukan s...

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Section: Cultivation Systemcontrasting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

Suwannarat¹,

Iewkittayakorn²,

Sukpondma³

et al. 2019

JSM

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“…In SSF, the optimum moisture content for growth and substrate utilization depends on the nature of the microorganism and the substrate used for cultivation. High initial moisture content leads to the accumulation of substrate particles, poor aeration, and possible anaerobic conditions, while very low moisture content restricts the fungal growth due to a reduction in solubility of the nutrients of the substrate (Kamath et al 2015). In this study, an initial moisture content of 70% was needed to yield the highest enzyme production of 19.82 U/gds (Fig.…”

Section: Effect Of Initial Moisture Contentmentioning

confidence: 77%

Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake

et al. 2017

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This study enhanced the production of thermostable organic solvent-tolerant (TS-OST) lipase by locally isolated thermotolerant sp. strain using solid-state fermentation (SSF) of palm kernel cake (PKC). The optimum conditions were achieved using a series of statistical approaches. The cultivation parameters, which include fermentation time, moisture content, temperature, pH, inoculum size, various carbon and nitrogen sources, as well as other supplements, were initially screened by the definitive screening design, and one-factor-at-a-time using PKC as the basal medium. Three significant factors (olive oil concentration, pH, and inoculum size) were further optimized using face-centred central composite design. The results indicated a successful and significant improvement of lipase activity by almost two-fold compared to the initial screening production. The findings showed that the optimal conditions were 2% (v/w) inoculum size, 2% (v/w) olive oil, 0.6% (w/w) peptone, 2% (v/w) ethanol, 70% moisture content at initial pH 10.0 and 45 °C within 72 h of fermentation. Process optimization resulted in maximum lipase activity of 58.63 U/gram dry solids (gds). The analysis of variance showed that the statistical model was significant ( value <0.0001) and reliable with a high value of (0.98) and adjusted (0.96). This indicates a better correlation between the actual and predicted responses of lipase production. By considering this study, the low-cost PKC through SSF appears to be promising in the utilization of agro-industrial waste for TS-OST lipase production. This is because satisfactory enzyme activity could be attained that promises industrial applications.

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“…This strain was grown in several agro-based natural media to select the best substrate for increased yield of lovastatin (5). Genetic and bioinformatic analysis of the whole genome of the lovastatin-producing soil isolate A. terreus (AH007774) revealed the presence of the lovastatin gene cluster (6, 7).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Soil Fungus Aspergillus terreus (KM017963), a Potent Lovastatin Producer

2016

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Optimization of Culture Conditions for Maximal Lovastatin Production by Aspergillus terreus (KM017963) under Solid State Fermentation

Cited by 28 publications

References 10 publications

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake

Complete Genome Sequence of Soil Fungus Aspergillus terreus (KM017963), a Potent Lovastatin Producer

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