Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5

Kim, Mi-Hee; Kong, Yoon-Jung; Baek, Hong; Hyun, Hyung-Hwan

doi:10.1016/j.jbiotec.2005.06.022

Cited by 51 publications

(33 citation statements)

References 15 publications

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“…Moreover, optimum concentration of the carbon, nitrogen sources and growth factors for both biomass and antagonistic activity, obtained from the regression equations, was glucose 17.4 g/L, lactose 17 g/L, sodium chloride 16.9 g/L, ammonium chloride 3.3 g/L, and mineral salt solution 18.3 g/L. This observation suggested that the antagonistic activity was growth depended as suggested by Kim et al [19].…”

Section: Påêééåáåö=~åç=péäéåíáçå=çñ=kìíêáéåíë=supporting

confidence: 57%

“…and suggested it as novel 'antagonistic probiotic' for shrimp larval rearing systems. Antagonistic compound production in general is affected by composition of medium and therefore medium optimization has been found to be important for the enhancement of bacteriocin production [19]. Accordingly in the present study primary screening of nutrients was done by conventional screening method and the final optimization was conducted by means of central composite design of RSM.…”

Section: Obpriqp=^ka=afp`rppflk=mentioning

confidence: 99%

“…This indicated that lactose played an important role for both biomass production and antagonistic activity compared to glucose. Kim et al [19] reported that highest growth of a bacteriocin producing Micrococcus sp. was in a medium containing both lactose and glucose.…”

Section: Påêééåáåö=~åç=péäéåíáçå=çñ=kìíêáéåíë=mentioning

confidence: 99%

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Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design

Radhakrishnan

Jayaprakash

Philip

et al. 2007

Biotechnol. Bioprocess Eng.

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= ^Äëíê~Åí= A marine isolate of jáÅêçÅçÅÅìë MCCB 104 has been identified as an aquaculture probiotic antagonistic to sáÄêáç. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, sáÄêáç=Ü~êîÉóá, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L). © KSBB hÉóïçêÇëW=éêçÄáçíáÅ, ~åí~Öçåáëã, Micrococcus, êÉëéçåëÉ=ëìêÑ~ÅÉ=ãÉíÜçÇçäçÖó, ÅÉåíê~ä=ÅçãéçëáíÉ=ÇÉëáÖå, ~èì~ÅìäíìêÉ= = = = = fkqolar`qflk= Vibriosis has been recognized as the major systemic bacterial disease of shrimp and prawn larvae in hatcheries [1][2][3]. Administration of antibiotics, although recognized as one of the management measures, leads to multiple antibiotic resistance among aquaculture pathogens [1,4,5] and the same is likely to be transferred to human pathogens too. Therefore, as an alternative strategy in the management of vibrios, application of biological control agents, especially antagonistic probiotics has been suggested [6,7]. Micrococcus MCCB 104 isolated and identified in our previous work [8] as a novel antagonistic probiotic suitable for tropical culture sys- G`çêêÉëéçåÇáåÖ=~ìíÜçêTel: +91-484-2381120 Fax: +91-484-2381120 e-mail: bsingh@md3.vsnl.net.in † Present address: Centre for Bioseparation Technology, Vellore Institute of Technology, Vellore 632 014, Tamil Nadu, India tems was found to inhibit a range of vibrios. As the next step, optimization of carbon, nitrogen and other growth factors were to be accomplished for enhanced production of biomass and antagonistic activity under aerated conditions as the organism was a strict aerobe. This work was undertaken accordingly with the above objective by employing response surface methodology (RSM).RSM is an empirical statistical modeling technique employed for multiple regression analysis using quantitative data obtained from properly designed experiments to solve multivariable equations simultaneously. It is used for studying the effect of several factors influencing the responses by varying them simultaneously and carrying out a limited number of experiments and their by it can improve product yield and reduce process variability, time, cost etc. This technique has been used for designing media for the production bacteriocins [9], enzymes [10], antibiotics [11], polysaccharides [12], and organic acids [13]. This pape...

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Section: Påêééåáåö=~åç=péäéåíáçå=çñ=kìíêáéåíë=supporting

confidence: 57%

Section: Obpriqp=^ka=afp`rppflk=mentioning

confidence: 99%

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Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design

Radhakrishnan

Jayaprakash

Philip

et al. 2007

Biotechnol. Bioprocess Eng.

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“…That the production of bacteriocin is closely related with bacterial growth of producing organism and bacteriocin activity decreases more or less sharply at the end of the growth phase as a result of degradation by proteases was reported earlier in Micrococcus sp. 26 . All the strains of Rhizobium were found to produce antimicrobial activity, which inhibited the growth of the related strains on the agar medium.…”

Section: Resultsmentioning

confidence: 99%

Production Bacteriosin by Rhizobia obtained from Root nodules of Macrotyloma uniflorum (Lam.) Verdc. (Horse Gram)

Edulamudi

Johnson

Divi

et al. 2012

Bangla. J. Microbiol.

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Thirty two Rhizobium isolates obtained from the root nodules of Horse gram plants grown in 32 different soil samples collected from various regions in Andhra Pradesh, India. They were identified as rhizobia by nodulation test. These isolates were used to study the production of bacteriocins. All the isolates produced bacteriocins against the remaining isolates. The isolates HGR-4 and 9 showed maximum activity/inhibition zone among all the isolates, and also found to be inhibiting more number of isolates than that of other bacteriocin producing isolates. The bacteriocin protein substance produced by the isolates HGR-4 and 9 showed a molecular weight of ~ 50 kDa by SDS-AGE and bacteriocin plasmids molecular weight is > 22 kb and > 24 kb.

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“…Em ambientes rico em nutrientes para sua multiplicação ou na ausência de microbiota competitiva é de se esperar que a produção de bacteriocinas seja diminuída ( VAN DEN BERGHE et al, 2006) Sabe-se que o valor do pH é importante para a multiplicação de bactérias láticas e a produção de bacteriocina. Dependendo do pH, ocorre a adsorção da bacteriocina à célula bacteriana, ou a secreção para o meio, onde a degradação proteolítica pode afetar sua atividade (KIM et al, 2006). Neste estudo, foi observado que a produção das bacteriocinas por L. sakei 2a foi maior nos modelos que continham 0,5% de glicose (formulações 3 e 4), nos quais o pH atingiu o valor 5, inferior ao atingido nos demais modelos.…”

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Avaliação da ação dos ingredientes da matriz alimentar na atividade antilisteria das bacteriocinas produzidas por Lactobacillus sakei subsp. sakei 2a

Barbosa¹

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Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5

Cited by 51 publications

References 15 publications

Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design

Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design

Production Bacteriosin by Rhizobia obtained from Root nodules of Macrotyloma uniflorum (Lam.) Verdc. (Horse Gram)

Avaliação da ação dos ingredientes da matriz alimentar na atividade antilisteria das bacteriocinas produzidas por Lactobacillus sakei subsp. sakei 2a

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