Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design

Radhakrishnan, P.; Jayaprakash, N. S.; Philip, Rosamma; Singh, I. S. Bright

doi:10.1007/bf02931353

Cited by 18 publications

(5 citation statements)

References 20 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to Vibrio (Preetha et al . ). Fishes can modulate their digestive enzyme secretion in response to the source of nutrients and concentration (Rodiles et al .…”

Section: Introductionmentioning

confidence: 97%

Effect of probiotics on digestive enzyme activities and growth of cichlids,Etroplus suratensis(Pearl spot) andOreochromis mossambicus(Tilapia)

Sankar

Philip

et al. 2016

Aquacult Nutr

View full text Add to dashboard Cite

In aquaculture, exposure to stressful conditions and problems related to diseases often occur resulting in serious economic loss. The use of probiotics that control pathogens through different mechanisms, aiding digestion by exoenzyme supply and establishment of beneficial microflora in the digestive tract has got increased attention recently. Two different bacterial species Micrococcus MCCB 104 and Bacillus MCCB 101 were tested as probiotics for two cichlid species Etroplus suratensis and Oreochromis mossambicus. The strains were administered separately and together, at a rate of 103 CFU animal−1 day−1 for 28 days. The effects on digestive enzyme activity and growth were compared with those fed on control diet. FCR of E. suratensis was high in case of Bacillus alone as well as the mixed culture (Micrococcus and Bacillus together) than that of control. However, the SGR of E. suratensis was slightly lower than that of control during 14 days trial. SGR of both the treatments (Bacillus alone and mixed culture) was higher than that of the control on 28th day, and this might be due to the reason that specific duration is needed for the assimilation and conversion of nutrients into proteins. The intestinal digestive hydrolases of E. suratensis were profoundly enhanced mainly by Bacillus and mixed cultures. The hepatopancreatic versions of major digestive enzymes in E. suratensis were influenced variously by different probiotic treatments. The alpha‐amylase activity of hepatopancreas has been elevated significantly by mixed culture during 14 days experiments while on 28 days trials both Bacillus and mixed culture showed an enhancing effect. During 14 days feeding trials, the intestinal lipase activity has been prominently enhanced by all probiotic treatments. It is found that, in O. mossambicus, both 14 days and 28 days feeding experiments, the pepsin as well as intestinal digestive enzymes such as amylase, total alkaline protease, lipase and their hepatopancreatic counter parts showed a significant elevation in their specific activity. Intestinal alpha‐amylase and total alkaline proteases of O. mossambicus showed an increase in specific activities on both 14 days and 28 days feeding experiments with the Bacillus and Micrococcus combination. The hepatopancreatic enzymes were highly influenced by the Bacillus species in O. mossambicus. The results of this study suggest that live probiotic microorganisms such as Micrococcus MCCB 104 and Bacillus MCCB 101 may be incorporated while formulating the cost‐effective, nutritionally balanced diet of E. suratensis and O. mossambicus for better growth performance and nutrient utilization.

show abstract

Section: Introductionmentioning

confidence: 97%

Effect of probiotics on digestive enzyme activities and growth of cichlids,Etroplus suratensis(Pearl spot) andOreochromis mossambicus(Tilapia)

Sankar

Philip

et al. 2016

Aquacult Nutr

View full text Add to dashboard Cite

show abstract

“…Production of aquaculture probiotic Micrococcus MCCB 104 was succeeded, using central composite design (Kim et al, 2004). Moreover, Preetha et al (2007) also described the optimization of medium composition for growth of Leuconostoc citreum.…”

Section: Introductionmentioning

confidence: 99%

Medium component improvement for β-galactosidase production by a probiotic strain Lactobacillus fermentum CM33

Sriphannam¹,

Unban²,

Ashida³

et al. 2012

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Plackett and Burman statistical design was applied to screen the nutritional factors affecting β-galactosidase production by Lactobacillus fermentum CM33. Accordingly, lactose, tryptone and Tween80 were found to be the positive effective factors at the significant level above 80%. Central composite design (CCD) and response surface plot predicted that the maximum enzyme activity of 49.81 U/100 ml would be obtained at 3.23 (w/v) lactose, 4.91% (w/v) tryptone and 0.62% (w/v) Tween80 and a trial experiment validated the activity total at 114% at 24 h of cultivation. However, the maximum activity of 63.31 U/100 ml culture was found at 16 h. The initial pH range 6.0 to 6.5 was found to be the most suitable for enzyme production. The final stage of optimization increased the productivity by 14.60 folds over that obtained with the non-optimized medium. The replacing of the expensive ingredients such as yeast extract, peptone and beef extract composed in the optimized medium by skimmed milk, did not significantly alter the enzyme productivity.

show abstract

“…Several previous studies have reported the precedence of single-factor analysis before using PBD and BBD [ 22 , 23 ]. Accordingly, initial screening was performed for the selection of optimum basal culture medium, wherein 2 × YT was found to be advantageous for the yield of plasmid DNA vaccine pcDNA-CCOL2A1 produced by the engineered E. coli DH5α.…”

Section: Resultsmentioning

confidence: 99%

Optimization of fermentation conditions for an Escherichia coli strain engineered using the response surface method to produce a novel therapeutic DNA vaccine for rheumatoid arthritis

et al. 2018

View full text Add to dashboard Cite

BackgroundFermentation condition optimization and nutrients screening are of equal importance for efficient production of plasmid DNA vaccines. This directly affects the downstream purification and final quality and yield of plasmid DNA vaccines. The present study aimed to optimize the fermentation conditions for high-throughput production of therapeutic DNA vaccine pcDNA-CCOL2A1 by engineered Escherichia coli DH5α, using the response surface method (RSM).ResultsWe hypothesized that optimized fermentation conditions significantly increase the yield of pcDNA-CCOL2A1 therapeutic DNA vaccine, a novel DNA vaccine for treating rheumatoid arthritis (RA). Single-factor analysis was performed to evaluate the optimal basal culture medium from LB, 2 × YT, TB, M9 (Glycerol) and M9 (Glucose), respectively. Thereafter, the Plackett-Burman design (PBD) was used to ascertain the three most significant factors affecting the vaccine yields, followed by the paths of steepest ascent to move to the nearest region of maximum response. Initial screening through the PBD revealed that the most key factors were peptone, mannitol, and inoculum concentration. Subsequent use of RSM was further optimized for the production of therapeutic DNA vaccine pcDNA-CCOL2A1 through Box-Behnken design (BBD). The final optimized fermentation conditions were as follows: peptone, 25.86 g/L; mannitol, 8.08 g/L; inoculum concentration, OD = 0.36. Using this statistical experimental design, the yield of therapeutic DNA vaccine pcDNA-CCOL2A1 markedly increased from 223.37 mg/L to339.32 mg/L under optimal conditions, and a 51.9% increase was observed compared with the original medium.ConclusionsThe present results provide a basis for further production of high-quality and high-yield therapeutic DNA vaccine pcDNA-CCOL2A1 in pilot-scale and even industrial-scale.

show abstract

Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design

Cited by 18 publications

References 20 publications

Effect of probiotics on digestive enzyme activities and growth of cichlids,Etroplus suratensis(Pearl spot) andOreochromis mossambicus(Tilapia)

Effect of probiotics on digestive enzyme activities and growth of cichlids,Etroplus suratensis(Pearl spot) andOreochromis mossambicus(Tilapia)

Medium component improvement for β-galactosidase production by a probiotic strain Lactobacillus fermentum CM33

Optimization of fermentation conditions for an Escherichia coli strain engineered using the response surface method to produce a novel therapeutic DNA vaccine for rheumatoid arthritis

Contact Info

Product

Resources

About