Optimization of conteining dermal coverages production for burn wound treatment at in vivo model

Abstract

Aim. The aim was to estimate the influence of cell suspension quality on the therapeutic efficiency of cell-containing dermal coverages in animal model in vivo. Methods. We carried out the application of gel wound coverages with different quality of cellular compound on the third degree burns of ICR line mice. In the negative control group animals were treated with fresh medium-containing gel, and in positive control – by gel containing high quality cell suspension. Photo fixation of burn wound status was carried out once a day. The results were estimated by ANOVA approach. Results. There was a statistically significant difference of burn wounds development and healing between positive control and both experimental groups. The decreasing of alive cell fraction in prepared gel within 5 hours has been shown as well. Conclusions. It has been shown the dependence of wound healing properties of coatings containing cells on the cell compound quality, in particular, from the viability and integrity of cells. These results are important for developing of clinical protocols using such cell‑containing dermal equivalents.
 Keywords: burn wound, dermal equivalent, stem cells, skin substitute, tissue engineering.

From mesenchymal stem cells to their secretoms as basic components of dermal coverings for the treatment of massive burns

“…The culture medium conditioned by stem cells looks even more attractive from a therapeutic point of view, as it affects through paracrine mechanisms [48][49][50]. In addition, accor ding to our data as well as according to other authors, the cultural medium conditioned by stem cells has antibacterial properties [19,51]. Back in 2010, British scientists [52] using the "scratch assays" method tested the in vitro biological activity of a cell-free medium conditioned by MSCs.…”

“…Although the average wound healing time in this group was not statistically significantly different from the similar indicator of the control group, but average wound condition was the best. As for the change in the size of the wound, here the greatest effectiveness was also observed with the preparation that carried li ving 4BL cells and did not contain cell lysate (impact index η 2 = 0.60, p ≤ 0.05) [19].…”

“…Consequently, we decided to test our hypothesis regarding the possibility of equal therapeutic potential in living cells and cell lysate [19]. For this purpose, we conducted two experiments with the involvement of two types of laboratory animals: the first, a pilot one -with the involvement of a small number of rats, and the next one -with the involvement of mice in a larger number.…”

“…Ensuring reliable fixation of the cell suspension on the wound surface could be achieved due to the fact that the cells will be immobilized on the surface of a filmlike wound co ve ring (for example, on polyacrylamide or collagen membranes) or distributed in a soft pharmaceutical form such as an ointment [16,[19][20][21]. In our opinion, the advantages of ointment-like substances for closing wound surfaces are:…”

“…Therefore, due to use of in vivo animal models later we decided to change slightly the direction of our research, focusing on the use of soft pharmaceutical forms of the ointment type separately from the solid carrier. The analysis of literature data led us to draw the following conclusion: it is the carbopol hydrogel based on the gelling agent Carbopol 980 with a carbopol concentration of 1.5 % that will serve as a soft pharmaceutical form in our experiments with model animals [16,[19][20][21].…”