Optimization of conditions for the extraction of antioxidants from solid parts of medicinal plants

Gribova, N. Yu.; Filippenko, T. A.; Nikolaevskii, A. N.; Belaya, N. I.; Tsybulenko, A. A.

doi:10.1134/s1061934808110038

Cited by 19 publications

(8 citation statements)

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“…The extraction yield of total saponins increased significantly when the ethanol concentration varied from 60% to 70%, while the yield increased little when concentration was increased above 70%. On the basis of the theory of similarity and intermiscibility, when polarities of solvent and solute are similar, the solute is easily dissolved from plant cells (Gribova and others 2008). In this experiment, when the ethanol concentration reached 70%, it got a maximum yield.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Ultrasonic‐Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

Hu¹,

Guo²,

Zhou³

et al. 2012

Journal of Food Science

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Eclipta prostrasta L. is a traditional Chinese medicine herb, which is rich in saponins and has strong antiviral and antitumor activities. An ultrasonic-assisted extraction (UAE) technique was developed for the fast extraction of saponins from E. prostrasta. The content of total saponins in E. prostrasta was determined using UV/vis spectrophotometric methods. Several influential parameters like ethanol concentration, extraction time, temperature, and liquid/solid ratio were investigated for the optimization of the extraction using single factor and Box-Behnken experimental designs. Extraction conditions were optimized for maximum yield of total saponins in E. prostrasta using response surface methodology (RSM) with 4 independent variables at 3 levels of each variable. Results showed that the optimization conditions for saponins extraction were: ethanol concentration 70%, extraction time 3 h, temperature 70 °C, and liquid/solid ratio 14:1. Corresponding saponins content was 2.096%. The mathematical model developed was found to fit well with the experimental data. Practical Application: Although there are wider applications of Eclipta prostrasta L. as a functional food or traditional medicine due to its various bioactivities, these properties are limited by its crude extracts. Total saponins are the main active ingredient of E. prostrasta. This research has optimized the extraction conditions of total saponins from E. prostrasta, which will provide useful reference information for further studies, and offer related industries with helpful guidance in practice.

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Section: Resultsmentioning

confidence: 99%

Optimization of Ultrasonic‐Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

Hu¹,

Guo²,

Zhou³

et al. 2012

Journal of Food Science

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“…However, further increasing the concentration of ethanol from 85% to 95% resulted in a decrease in the content of steroidal saponins. The reason for this phenomenon might be attributed to the theory of similarity and intermiscibility; if the polarities of the solvent and solute are similar, the solute is easily dissolved from plant cells [ 30 ]. As a result, an ethanol fraction of 85% ( v / v ) was selected as the optimal volume fraction for the extraction of steroidal saponins.…”

Section: Resultsmentioning

confidence: 99%

Optimization of the Ultrasonic-Assisted Extraction Technology of Steroidal Saponins from Polygonatum kingianum Collett & Hemsl and Evaluating Its Quality Planted in Different Areas

Wang

Chen

et al. 2022

Molecules

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Polygonatum kingianum Collett & Hemsl is one of the famous traditional Chinese herbs with satisfactory therapeutic effects on invigorating Qi, nourishing Yin and moistening lungs, in which steroidal saponins are one class of important active substances. The main purpose is to determine the optimal extraction technology of steroidal saponins and evaluate the quality of P. kingianum planted in five different areas. The optimal ultrasonic-assisted extraction (UAE) technology was established by using single-factor experiments and the response surface methodology (RSM), and the determination method of high-performance liquid chromatography (HPLC) for dioscin and diosgenin, two primary types of acid-hydrolyzed steroidal saponins, was constructed with good linear range and precision. The results showed that UAE was an efficient extraction method for steroidal saponins, and the extraction yield was significantly affected by the liquid-solid ratio. The optimal extraction technology was generated following a liquid-solid ratio of 10:1 (mL/g), an ethanol concentration of 85% (v/v), an extraction time of 75 min, an extraction temperature of 50 °C and three extractions, of which these parameters were in line with the predicted values calculated by RSM. Considering only dioscin and diosgenin, the quality of P. kingianum planted at five sample plots presented non-significant difference. However, the content of diosgenin in Pingbian Prefecture (PB) was higher than that of the other four areas with a value of 0.46 mg/g. Taken together, the optimal UAE technology for P. kingianum steroidal saponins was determined via RSM. The quality evaluation revealed that there was a non-significant difference among P. kingianum planted in different areas based on the contents of the sum of dioscin and diosgenin. This work has important reference value for the exploitation and utilization of P. kingianum.

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“…Even lower yields than the maceration at room temperature were achieved by sonication. Generally, sonication is efficient and rapid extraction method [ 91 , 92 ]. Using ultrasonic waves we observed relatively low yield for protocatechuic acid, vanillin and quercitrin ( Figure 4 B).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Various Easy-to-Use Procedures for Extraction of Phenols from Apricot Fruits

et al. 2011

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Phenols are broadly distributed in the plant kingdom and are the most abundant secondary metabolites of plants. Plant polyphenols have drawn increasing attention due to their potential antioxidant properties and their marked effects in the prevention of various oxidative stress associated diseases such as cancer. The objective of this study was to investigate a suitable method for determination of protocatechuic acid, 4-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferulic acid, quercetin, resveratrol and quercitrin from apricot samples. A high-performance liquid chromatograph with electrochemical and UV detectors was used. The method was optimized in respect to both the separation selectivity of individual phenolic compounds and the maximum sensitivity with the electrochemical detection. The lowest limits of detection (3 S/N) using UV detection were estimated for ferulic acid (3 µM), quercitrin (4 µM) and quercetin (4 µM). Using electrochemical detection values of 27 nM, 40 nM and 37 nM were achieved for ferulic acid, quercitrin and quercetin, respectively. It follows from the acquired results that the coulometric detection under a universal potential of 600 mV is more suitable and sensitive for polyphenols determination than UV detection at a universal wavelength of 260 nm. Subsequently, we tested the influence of solvent composition, vortexing and sonication on separation efficiency. Our results showed that a combination of water, acetone and methanol in 20:20:60 ratio was the most effective for p-aminobenzoic acid, chlorgenic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, resveratrol and quercetin, in comparison with other solvents. On the other hand, vortexing at 4 °C produced the highest yield. Moreover, we tested the contents of individual polyphenols in the apricot cultivars Mamaria, Mold and LE-1075. The major phenolic compounds were chlorgenic acid and rutin. Chlorgenic acid was found in amounts of 2,302 mg/100 g in cultivar LE-1075, 546 mg/100 g in cultivar Mamaria and 129 mg/100 g in cultivar Mold. Generally, the cultivar LE-1075 produced the highest polyphenol content values, contrary to Mold, which compared to cultivar LE-1075 was quite poor from the point of view of the phenolics content.

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Optimization of conditions for the extraction of antioxidants from solid parts of medicinal plants

Cited by 19 publications

References 0 publications

Optimization of Ultrasonic‐Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

Optimization of Ultrasonic‐Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

Optimization of the Ultrasonic-Assisted Extraction Technology of Steroidal Saponins from Polygonatum kingianum Collett & Hemsl and Evaluating Its Quality Planted in Different Areas

Comparison of Various Easy-to-Use Procedures for Extraction of Phenols from Apricot Fruits

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