Optimization of chromium and tannic acid bioremediation by Aspergillus niveus using Plackett–Burman design and response surface methodology

Chaudhary, Prachi; Chhokar, Vinod; Choudhary, Pooja; Kumar, Anil; Beniwal, Vikas

doi:10.1186/s13568-017-0504-0

Cited by 18 publications

(10 citation statements)

References 44 publications

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“…However, in traditional ‘one-factor-at-a-time’ experiments, the effects of various factors can only be investigated one at a time, and this approach fails to evaluate multifactorial interactions among all components, thereby leading to inefficient and time-consuming work (Lee 2018). Response surface methodology (RSM) includes factorial designs and regression analysis for construction of empirical models, making it an excellent statistical tool for increasing the production of valuable metabolites (Chaudhary et al 2017; Fu et al 2016). RSM can evaluate all the factors simultaneously and determine the optimal culture conditions for microbes.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

Shen

et al. 2019

AMB Expr

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Ascomycin, a key intermediate for chemical synthesis of immunosuppressive drug pimecrolimus, is produced by Streptomyces hygroscopicus var. ascomyceticus . In order to improve the strain production, the original S. hygroscopicus ATCC 14891 strain was treated here with atmospheric and room temperature plasma to obtain a stable high-producing S. hygroscopicus SFK-36 strain which produced 495.3 mg/L ascomycin, a 32.5% increase in ascomycin compared to the ATCC 14891. Then, fermentation medium was optimized using response surface methodology to further enhance ascomycin production. In the optimized medium containing 81.0 g/L soluble starch, 57.4 g/L peanut meal, and 15.8 g/L soybean oil, the ascomycin yield reached 1466.3 mg/L in flask culture. Furthermore, the fermentation process was carried out in a 5 L fermenter, and the ascomycin yield reached 1476.9 mg/L, which is the highest ascomycin yield reported so far. Therefore, traditional mutagenesis breeding combined with medium optimization is an effective approach for the enhancement of ascomycin production. Electronic supplementary material The online version of this article (10.1186/s13568-019-0749-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

Shen

et al. 2019

AMB Expr

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“…At the same time, the adjusted determination coefficient (Adj R-squared, Table 9) (0.82 > 0.80) and coefficient of variance (CV%) was 9.19, which further indicated that the multivariate regression relationship between the dependent variable and the all independent variables was significant [31]. In other words, the regression equation was adequate in predicting the optical density (OD600) under different fermentation conditions [32] ( Figure 6). The profiles of the response surfaces between fermentation time and inoculum level, fermentation time and pH, inoculum level and pH were all convex with an open downward direction, indicating a parabolic relationship between the OD600 value and the three factors of fermentation time, inoculum level and pH [33].The surface plots showed the interactive effects of the three significant fermentation factors on the bacterial growth.…”

Section: Optimization: Box-behnken Designmentioning

confidence: 79%

Section: Optimization: Box-behnken Designmentioning

confidence: 80%

Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

Yang

Guo

et al. 2020

IJERPH

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(1) Objective: The objective of this study was to screen amoxicillin (AMX)-degrading bacterial strains in pig manure and optimize the fermentation conditions for these strains to achieve high fermentation rate, which can provide an effective way for the practical application of bacterial strains as antibiotic-degrading bacterial in treating livestock waste for antibiotic residues. (2) Methods: Antibiotic susceptibility tests and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were employed to screen AMX-degrading bacterial strains in pig manure. The culture conditions were optimized for AMX-degrading bacterial strains using Plackeet–Burman design (PBD), the steepest ascent design, and the response surface methods, coupled with the Box–Behnken design (BBD). The effects of culture time, temperature, rotator (mixing) speed, inoculum level, and initial pH value on the growth of AMX-degrading strains were investigated. Experimental data obtained from BBD were utilized to generate a second-order polynomial regression model for evaluating the effects of the tested variables on the optical density at 600 nm (OD600) of culture solutions as the growth indicator for the screened AMX-degrading strains. (3) Results: The initial pH, culture time, and the inoculum level had significant effects on the OD600 value (growth) of the screened AMX-degrading strains. The initial pH value was found to be the most critical factor influencing the growth of bacteria. The optimized culture condition for the bacterial growth determined by the response surface methodology was: the initial pH of 6.9, culture time of 52 h, and inoculum level of 2%. The average OD value of 12 different fermentation conditions in the initial fermentation tests in this study was 1.72 and the optimization resulted in an OD value of 3.00. The verification experiment resulted in an OD value of 2.94, which confirmed the adequacy of the optimization model for the determining the optimal culture condition. (4) Conclusions: The growth of the screened strain of AMX-degrading bacteria could be optimized by changing the fermentation conditions. The optimization could be achieved by using the Box–Behnken response surface method and Plackett–Burman experimental design.

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“…By moving each variable from the chosen reference point while keeping the other variables at constant reference values, the response changes are presented in the perturbation plot. 50 As shown in Figure S2 , the curve with the most notable change was the fermentation period (A) followed by culture temperature (B). The least notable variable was defined as antifoam reagent addition (C).…”

Section: Resultsmentioning

confidence: 99%

Development of a New Bioprocess for Clean Diosgenin Production through Submerged Fermentation of an Endophytic Fungus

et al. 2021

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Diosgenin is used widely to synthesize steroidal hormone drugs in the pharmaceutical industry. The conventional diosgenin production process, direct acid hydrolysis of the root of Dioscorea zingiberensis C. H. Wright (DZW), causes large amounts of wastewater and severe environmental pollution. To develop a clean and effective method, the endophytic fungus Fusarium sp. CPCC 400226 was screened for the first time for the microbial biotransformation of DZW in submerged fermentation (SmF). Statistical design and response surface methodology (RSM) were implemented to develop the diosgenin production process using the Fusarium strains. The environmental variables that significantly affected diosgenin yield were determined by the two-level Plackett–Burman design (PBD) with nine factors. PBD indicates that the fermentation period, culture temperature, and antifoam reagent addition are the most influential variables. These three variables were further optimized using the response surface design (RSD). A quadratic model was then built by the central composite design (CCD) to study the impact of interaction and quadratic effect on diosgenin yield. The values of the coefficient of determination for the PBD and CCD models were all over 0.95. P -values for both models were 0.0024 and <0.001, with F -values of ∼414 and ∼2215, respectively. The predicted results showed that a maximum diosgenin yield of 2.22% could be obtained with a fermentation period of 11.89 days, a culture temperature of 30.17 °C, and an antifoam reagent addition of 0.20%. The experimental value was 2.24%, which was in great agreement with predicted value. As a result, over 80% of the steroidal saponins in DZW were converted into diosgenin, presenting a ∼3-fold increase in diosgenin yield. For the first time, we report the SmF of a Fusarium strain used to produce diosgenin through the microbial biotransformation of DZW. A practical diosgenin production process was established for the first time for Fusarium strains. This bioprocess is acid-free and wastewater-free, providing a promising environmentally friendly alternative to diosgenin production in industrial applications. The information provided in the current study may be applicable to produce diosgenin in SmF by other endophytic fungi and lays a solid foundation for endophytic fungi to produce natural products.

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Optimization of chromium and tannic acid bioremediation by Aspergillus niveus using Plackett–Burman design and response surface methodology

Cited by 18 publications

References 44 publications

Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

Development of a New Bioprocess for Clean Diosgenin Production through Submerged Fermentation of an Endophytic Fungus

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