Optimization of cellulase production by a brown rot fungus Fomitopsis sp. RCK2010 under solid state fermentation

Deswal, Deepa; Khasa, Yogender Pal; Kuhad, Ramesh Chander

doi:10.1016/j.biortech.2011.03.032

Cited by 241 publications

(121 citation statements)

References 33 publications

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“…All nitrogen sources tested promoted BGL production, but organic nitrogen sources were found to be especially favorable for cell growth and cellulase production. This result is in agreement with many reports in the literature (Haltrich et al 1994;Deswal et al 2011;Gautam et al 2011). Peptones have been found to support maximum cellulase production by Trichoderma reesei (Enari and Markkanen 1977), and they are known to be suitable nitrogen sources, especially soy peptone (Baig 2005).…”

Section: Effect Of Carbon Source Nitrogen Source and Vitaminsupporting

confidence: 91%

“…Many researchers have reported that the presence of vitamins influences the rate of biosynthesis of many metabolites and increases enzyme activities (Báez-Saldaña et al 2004;Chen et al 2004;Deswal et al 2011;Lee et al 2001). Additionally, vitamins are easy to deliver during liquid fermentation (Joo et al 2009).…”

Section: Effect Of Carbon Source Nitrogen Source and Vitaminmentioning

confidence: 99%

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Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Lee¹,

Lee²,

Kim³

et al. 2014

BioResources

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Medium components for β-glucosidase (BGL) production in Schizophyllum commune KUC9397 were optimized using a central composite design and response surface methodology. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optimal carbon, nitrogen, and vitamin sources, respectively. The highest BGL production was obtained with 2.96% cellulose, 2.30% soy peptone, and 0.11% thiamine HCl. BGL production in the optimized medium was increased 7.2-fold compared to production in an unoptimized medium. Crude enzyme preparation from S. commune KUC9397 was used to saccharify pretreated lignocellulosic biomass. The crude enzyme preparations showed statistically equal saccharification rates as Cellobiase, a commercial BGL. This finding indicates that crude enzymes produced by S. commune KUC9397 have good potential for application in cellulosic biomass conversion systems in place of Cellobiase.

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Section: Effect Of Carbon Source Nitrogen Source and Vitaminsupporting

confidence: 91%

Section: Effect Of Carbon Source Nitrogen Source and Vitaminmentioning

confidence: 99%

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Lee¹,

Lee²,

Kim³

et al. 2014

BioResources

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“…The major problem is cost factor of exploitation of cellulase, with contribute as much as 50 % to the overall cost [4]. In order to improve the yields of cellulase, lots of work has been made in developing new strains, optimizing culture conditions [5][6][7][8], and cultivation mode [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108

et al. 2012

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Trichoderma reesei YC-108, a strain isolated by a kind of newly invented plate was found to over produce cellulase and it was then used as a cellulase producer. To get the maximum amount of cellulase, the combination of the medium ingredients, which has a profound influence on metabolic pathway was optimized using response surface methodology. The optimum composition was found to be 24.63 g/L wheat bran, 30.78 g/L avicel, and 19.16 g/L soya-bean cake powder. By using the optimized medium, the filter paper activity (FPA) increased nearly five times to 15.82 IU/mL in a 30 L stirred fermenter, carboxymethyl cellulase activity (CMCase) was increased from 83.02 to 628.05 IU/mL and the CMCase/FPA ratio was nearly doubled compared with the parent strain at initial medium.

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“…The synergistic action of cellulase and xylanase enzymes is required for the bioconversion of cellulose and hemicellulose into fermentable sugars. Endoglucanase act randomly on the insoluble and soluble chains of cellulose, while exoglucanase liberate cellobiose from the ends of cellulose chains (reducing and non-reducing) and ᵦ-glucosidases will further liberate glucose from cellobiose [30,31]. Xylanase catalysis xylan hydrolysis to produce a mixture of xylose, xylobiose and shorter xylo-oligosaccharides [32].…”

Section: Resultsmentioning

confidence: 99%

Saccharification of Lignocellulosic Materials by Cellulolytic and Xylanolytic <i>Paenibacillus illioisensis </i>CX11

Ahmed

McKay

2017

AJAS

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The utilization of lignocellulosic materials to produce a variety of building blocks (e.g. fermentable sugars) is an interesting alternative approach to meeting the growing demand for high value chemicals. Cellulose and hemicellulose can be hydrolyzed by cellulase and xylanase enzymes into their respective building blocks (hexoses and pentoses), which can later be converted into the targeted compounds. The aim of this study was to test the ability of Paenibacillus illinoisensis CX11 to saccharify different lignocellulosic materials, and to determine its ability to produce cellulolytic and xylanolytic enzymes for possible use in converting lignocellulosic materials into their respective fermentable sugars. The ability of P. illinoisensis CX11 to produce CMCase, xylanase, FPase, and avicelase was tested using SSF of corn stalk. Furthermore, the ability of P. illinoisensis CX11 to saccharify lignocellulosic materials was tested using corn stalk, wheat bran, sawdust, and corn cob. The amount of reducing sugars released from the saccharification of lignocellulosic materials was determined by the 3,5-dinitro-salicylic acid (DNS) method. Obtained results showed that P. illinoisensis CX11 can produce CMCase (400.12 Â± 1.23 U/L), xylanase (385.57 Â± 2.25 U/L), FPase (266.93 Â± 2.22 U/L), avicelase (187.85 Â± 2.22 U/L) and extracellular protein (4.56 Â± 0.14 mg/L). Moreover, P. illinoisensis CX11 showed an ability to saccharify lignocellulosic materials. These findings confirm that P. illinoisensis CX11 has the ability to produce cellulolytic and xylanolytic enzymes, and to hydrolyze different lignocellulosic materials into fermentable sugars. Therefore, this study concludes that P. illinoisensis CX11 can be considered a good source of cellulase and xylanase enzymes to saccharify different lignocellulosic materials.

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Optimization of cellulase production by a brown rot fungus Fomitopsis sp. RCK2010 under solid state fermentation

Cited by 241 publications

References 33 publications

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108

Saccharification of Lignocellulosic Materials by Cellulolytic and Xylanolytic <i>Paenibacillus illioisensis </i>CX11

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