A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108

Zhang, Yan; Cao, Xiaohong; Liu, Qingdai; Yang, Zaiyue; Teng, Yuou; Zhao, Juan

doi:10.1007/s12088-012-0311-x

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…To the best of our knowledge, this is the first report suggesting that MM + wheat bran-2 medium could increase the nematicidal activity of T6 culture filtrates against H. avenae J2s. This is consistent with the previous study of the fermentation medium and fermentation parameters in enhancing the bio-control ability of T. viride YC-108 mutant strain (Yan et al, 2012;Yang et al, 2016). Yan et al (2012) reported that wheat bran, avicel, and soya-bean cake powder, nitrogen source, and soybean meal could promote and increase the cellulase production from the strain of T. reesei YC-108.…”

Section: Discussionsupporting

confidence: 93%

“…This is consistent with the previous study of the fermentation medium and fermentation parameters in enhancing the bio-control ability of T. viride YC-108 mutant strain (Yan et al, 2012;Yang et al, 2016). Yan et al (2012) reported that wheat bran, avicel, and soya-bean cake powder, nitrogen source, and soybean meal could promote and increase the cellulase production from the strain of T. reesei YC-108. In addition, Wang et al (2014) revealed that the components of K 2 HPO 4 , beef extract, beef peptone, and glucose in a liquid fermentation medium improved the nematicidal activity of the nematode pathogenic strain Xenorhabdus nematophila HB301.…”

Section: Discussionsupporting

confidence: 93%

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Optimization of the Fermentation Media and Parameters for the Bio-control Potential of Trichoderma longibrachiatum T6 Against Nematodes

et al. 2020

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The cereal cyst nematode Heterodera avenae is one of the important soil-borne pathogens of cereal crops and causes high yield losses worldwide. Trichoderma spp. formulations are applied as commercial bio-control agents against soil-borne plant pathogens such as H. avenae . However, the relationship between Trichoderma longibrachiatum fermentation parameters and its bio-control potential against H. avenae has not been exclusively established. In the present study, the effect of 10 different fermentation media and conditions on the nematicidal activity of T. longibrachiatum T6 (T6) was evaluated with a single-factor method and a Plackett–Burman design, and the interaction between different fermentation parameters was investigated by a Box–Behnken design. The variables for enhancing the nematicidal activity of T6 culture filtrates were explored and optimized using response surface methodology (RSM). The Minor Medium (MM) plus wheat bran-2 medium was found to be the most effective fermentation medium for T6 culture filtrates against the second stage juveniles (J2s) of H. avenae . The maximum mortality of the J2s was obtained using the T6 culture filtrates under the following fermentation conditions: initial pH 6, 28°C culture temperature, 180 rpm rotating speed, 60 ml of fermentation media, 7 days of incubation time, and 1 ml of inoculation volumes. Among these parameters, the initial pH, inoculation volume, and incubation day were identified as the most significant parameters and critical independent variables for enhancing the nematicidal activity of the T6 culture filtrates. After further optimizations based on statistical predictions, the highest nematicidal activity (92.42%) was obtained with the T6 culture filtrates fermented under an initial pH of 6.06, an inoculation volume of 1.62 ml, and an incubation time of 7.15 days. The nematicidal activity was increased approximately by as high as 1.07% compared with that before optimization. Bio-control efficacy of T6 culture filtrates was 83.88% at the 70 th day after wheat seeds sowing in greenhouse experiments. The results from the validation experiments agreed with the model predictions. Our study has improved the bio-control potential of Trichoderma spp. against the plant-parasitic nematodes H. avenae and provided a cost-efficient bio-resource in the future development of novel bio-control agents.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Optimization of the Fermentation Media and Parameters for the Bio-control Potential of Trichoderma longibrachiatum T6 Against Nematodes

et al. 2020

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“…The filter paper activity from mutants obtained with variation of cellulosic substrates ranging from 0.73 to 1.09 FPU/mL. Yan et al (2012) reported that cellulase production using the mutant T. reesei YC-108 increased the FPU activity nearly five times to 15.82 IU/mL. Meanwhile, the mutan NU-6 showed approximately 1.8 fold increase activity of FPase (4.7 U/mL) after the strain of T. reesei Rut C-30 was treatment with nitrosoguanidine followed by UV irradiation (Jun et al, 2009).…”

Section: Cellulolytic Activity After Mutationmentioning

confidence: 99%

<i>ULTRAVIOLET MUTAGENESIS OF LOCAL ISOLATE Trichoderma sp. T065 FOR IMPROVING CELLULASES ACTIVITY </i>(Mutagenesis Isolat Lokal<i> Trichoderma sp. </i>T065 menggunakan Ultraviolet untuk meningkatkan Aktivitas Selulase)

Anindyawati¹,

Jusuf²,

Abimanyu

2016

J selulosa

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Mutagenesis of indigenous fungal isolates Trichoderma sp. T065 was achieved by UV light in a laminar air flow and UV crosslinker to increase cellulase activity. Thirty-four mutants were tested for their growth capacity in mineral agar with several carbon sources: Whatman filter paper no.1, 1% carboxymethylcellulose (CMC), 2% cellulose powder, 1% Avicel and 4% delignified oil palm empty fruit bunches (DOPEFB) with granule size of 200 mesh. Three mutants (UV-1.1, 1.2-UV and UV-1.3) showed bigger growth zone on cellulose substrate of 4% DOPEFB than that of wild type Trichoderma sp. T065. The highest cellulase activities were 0.65 FPU/mL and 0.57 FPU/mL from UV-1.1 and UV-1- 3, respectively higher than wild type that is equal to 0.038 FPU/mL.Keywords: Trichoderma sp. T065, mutations, UV light, carbon source, cellulase activityABSTRAKMutagenesis isolat lokal kapang Trichoderma sp. T065 dilakukan dengan sinar UV pada laminar air flow dan UV crosslinker untuk meningkatkan aktivitas selulase. Tiga puluh empat kapang mutan diuji kapasitas pertumbuhannya pada mineral agar dengan beberapa jenis sumber karbon yaitu kertas saring Whatman no.1, 1% carboxymethylcellulose (CMC), 2% serbuk selulosa, 1% avicel dan 4% tandan kosong sawit (TKS) dengan ukuran granula 200 mesh. Tiga mutan (UV-1.1, UV-1.2 dan UV- 1.3) mempunyai zona pertumbuhan yang lebih besar pada substrat selulosa dengan sumber karbon 4% TKS daripada isolat asli Trichoderma sp. T065. Aktivitas selulase tertinggi adalah 0,65 FPU/mL dan 0,57 FPU/mL berturut-turut dari mutan UV-1.1 dan UV-1.3 yang lebih tinggi dari isolat aslinya yaitu 0,038 FPU/mL.Kata kunci : Trichoderma sp. T065, mutasi, sinar UV, sumber karbon, aktivitas selulase

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“…The quadratic model has high F value (21.01) and low P value (0.0064). Sathish et al [8] and Yan et al [14] also noticed that quadratic model is best suitable model for analysis of the mixture design. The special cubic and cubic models have the higher R [11] the model which have the higher R value and lower P value are significant based on this the cubic and special cubic models were rejected and further analysis was carried out with the quadratic model only.…”

Section: Mixture Designmentioning

confidence: 99%

Intensification of Fructosyltransferases and Fructo-Oligosaccharides Production in Solid State Fermentation by Aspergillus awamori GHRTS

Sathish

Prakasham

2013

Indian J Microbiol

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The present work was aimed to investigate the impact of the solid substrates mixture on Fructosyltransferases (FTase) and Fructo-oligosaccharides (FOS) production. An augmented simplex lattice design was used to optimize a three component mixture for FTase production. Among selected substrates corn cobs has highest impact on FTase production followed by wheat bran and rice bran. All two substrates and three substrate combinations showed the highest enzyme production than their individual levels. Among the tested various models quadratic model was found to be the best suitable model to explain mixture design. Corncobs, wheat bran and rice bran in a ratio of approximately 45:29:26 is best suitable for the FTase production by isolated Aspergillus awamori GHRTS. This study signifies mixture design could be effective utilize for selection of best combination of multi substrate for improved production of high value products under solid state fermentation.

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A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108

Cited by 5 publications

References 16 publications

Optimization of the Fermentation Media and Parameters for the Bio-control Potential of Trichoderma longibrachiatum T6 Against Nematodes

Optimization of the Fermentation Media and Parameters for the Bio-control Potential of Trichoderma longibrachiatum T6 Against Nematodes

<i>ULTRAVIOLET MUTAGENESIS OF LOCAL ISOLATE Trichoderma sp. T065 FOR IMPROVING CELLULASES ACTIVITY </i>(Mutagenesis Isolat Lokal<i> Trichoderma sp. </i>T065 menggunakan Ultraviolet untuk meningkatkan Aktivitas Selulase)

Intensification of Fructosyltransferases and Fructo-Oligosaccharides Production in Solid State Fermentation by Aspergillus awamori GHRTS

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