Optimization of Cellulase Production by Aspergillus niger Isolated from Forest Soil

Abstract

Background:An impressive increase in the application of cellulases in various fields over the last few decades demands extensive research in improving its quality and large-scale production. Therefore, the current investigation focuses on factors relevant for optimal production of cellulase byAspergillus nigerisolated from forest soil.Method:Throughout this study, the fungal strainAspergillus nigerwas maintained under the submerged condition for a period of 7 days at 120 rpm rotational speed. Various physical and chemical conditions were employed in examining their influence on cellulase production by the selected fungal strain. After appropriate incubation, culture filtrates were withdrawn and checked for FPase, CMCase, and β-D-glucosidase activities.Results:The optimum pH and temperature for cellulase production were found to be 5.0 and 32°C, respectively. Among the various carbon sources tested in the present study, amendment of lactose in the medium yielded peak values of FPase (filter paperase) and CMCase (Carboxy-methyl cellulase) whereas fructose supported the higher titers of β-glucosidase. Among the nitrogen sources, profound FPase and CMCase activity were recorded when urea was used but higher β-glucosidase activity was noticed when yeast extract was added. Various natural lignocellulosic substrates like bagasse, coir, corncob, groundnut shells, litter, rice bran, rice husk, sawdust and wheat bran were tested to find out the induction of cellulase. Among the lignocelluloses, sawdust and litter served as good substrates for cellulase production byAspergillus niger.Conclusion:In gist, the outcome of this study sheds light on the cellulolytic potentiality of the fungal strainAspergillus nigerpromising in its future commercial applications which may be economically feasible.

Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation

“…The variation in pH causes loss in shape of enzyme because of changes in ionic bonding of enzyme (Mmango-Kaseke et al 2016). Akula and Golla (2018) reported pH 5 for optimal cellulase production which was comparable to our finding.…”

“…So there is a dire need to improve the economics of cellulase production either by reducing the productivity cost or enhancing the enzyme activities. The production cost is basically associated with the enzyme productivity of microbial strain as well as the type of fermentation media used (Akula and Golla 2018). Keeping in view the above consideration the current work focused on the process optimization for enhanced cellulase production by locally isolated fungal strain.…”

Cost‐effective cellulase production, improvement strategies, and future challenges

“…Examinations on extracellular fungal cellulases have been focused mostly on Trichoderma sp. recommended the opportunity for different cellulase methods might be used for hydrolysis of cellulose (Akula & Golla, 2018; Darmwal, Schraft, & Qin, 2009).…”

Application of Box–Behnken design for the optimization of cellulase production under solid-state fermentation

“…The maximum cellulase activities (IU/mL) in terms of FPA and CMCase attained by A. niger were 1.47 ± 0.08 and 11.02 ± 0.05 under 30% whey containing wheat bran based solid-state fermentation. Lactose sugars present in the whey may provide a favorable conditions for better cellulase activity for Aspergillus strain [29].…”