The most abundant biopolymer accessible on earth is lignocellulose. The hydrolysis of lignocelluloses is completed through enzyme groups such as ligninases, hemicelluloses, and cellulases as independently and together called lignocellulolytic enzymes. This review is focused on dealing with enhanced production of cellulose using modern techniques like strain improvement, consolidated bioprocessing, metabolic engineering, and recombinant technology, and defines the technology to boost the enzyme activity and its yield. Furthermore, the restrains linked with the production of cellulolytic enzyme and the direction of upcoming research to offer a complete strategy to improve cellulase yield with novel properties for industrial application at cost‐effective levels are mentioned.Practical applicationsCellulase is a complex enzyme consisting of an endoglucanase, exoglucanase, and β‐D‐glucosidase which acts in combination to release a small unit of glucose. Cellulase has vast potential in the industrial sector especially in the food and beverage as well as paper and pulp industries. The parameters such as strain improvement and heterologous gene expression are involved to enhance the cellulase production. This review discusses the cost‐effective production of cellulolytic enzyme and also emphasizes the limitations and future prospects of this enzyme production on a large scale.
Strain improvement through random mutagenesis is an extremely developed practice and it plays an important role in the economical growth of microbial agitation processes. The present study comprises genetic improvement of fungus isolated from petrol pump soil by ethyl methane sulfonate (EMS) mutagenesis for increased production of extracellular lipase. Random mutagenesis was performed by incubating the spore suspension of fungus with EMS at a concentration of 5% (v/v) and 8% (v/v) for 30, 60 and 90 minutes, respectively. Control set was prepared by incubating the spore suspension with sterile distilled water. Control plate showed maximum number of fungal colonies whereas number of colonies was decreased as we increased exposure time of EMS from 30 to 90 minutes. The lipase activity of six mutagenic strains and wild strain was determined under submerged fermentation and solid state fermentation. Treated culture named as EMS5%-60min (obtained after 60 minutes exposure with 5% EMS) exhibited maximum activity (32.09±1.84 IU/ml/min) in SmF as compared to wild strain (8.77±3.52 IU/ml/min) and another treated strain named as EMS8%-90min (obtained after 90 minutes exposure with 8% EMS) exhibited maximum activity (7.99±0.12 IU/g/min) in SSF as compared to wild strain (1.77±0.71 IU/g/min). The activity of mutagenic strain i.e. EMS5%-60min was increased to 365.90% as compared to 100% activity of wild strain in SmF whereas activity of another mutagenic strain i.e. EMS 8%-90min was increased to 451.41% as compared to 100% activity of wild strain in SSF.
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