Optimization of cell disruption methods for efficient recovery of bioactive metabolites via NMR of three freshwater microalgae (chlorophyta)

Ling, Nyuk; Teh, Kit Yinn; Lam, Su Shiung; Kaben, Anne Marie; San, Thye

doi:10.1016/j.biortech.2015.03.036

Cited by 11 publications

(5 citation statements)

References 25 publications

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“…The extraction of non-polar phases was made as described by Ma et al 20 , with some modifications. To 0.10 g of microalgal freeze dried biomass an aqueous solution (methanol:water in a 1:1 ratio) and chloroform in 1:1 ratio were added.…”

Section: Solvent Extractionmentioning

confidence: 99%

Exploring Pavlova pinguis chemical diversity: a potentially novel source of high value compounds

Fernandes

Martel

Cordeiro

2020

Sci Rep

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In the Haptophyta Pavlova pinguis J. C. Green 16 only specific classes of compounds have been analyzed to assess its potential as food for larval hatcheries in aquaculture and as ecological biomarkers 13,17,18. For instance, Milke, et al. 11 and Parrish, et al. 12 assessed the ability of P. pinguis and other Pavlova species to sustain postlarval sea scallop growth focusing on its proximate, fatty acid and sterol composition. In this microalga the complete characterization of lipid components (simple and complex lipids) is still largely unexplored 6,9. Thus, in the present study the analysis of the lipophilic fraction of P. pinguis was performed in order to identify its lipophilic features before and after alkaline hydrolysis through gas chromatography-mass spectrometry (GC-MS) and evaluate its prospects for further improvement in bioactive compounds. Materials and Methods Growth and culture conditions. The haptophyta Pavlova pinguis (RCC 1539) was obtained from the Roscoff Culture Collection (RCC). The microalgal cultures were made by inoculating starter cultures into 1L of sterile f/2-Si medium with pH adjusted to 7.0 under 70 μmol m −2 s −1 light intensity with 16:8 h (light: dark cycles) at 25 °C. At the end of the logarithmic phase, the medium was centrifuged for 7 min. at 3720 g and the pellets washed. Microalgae growth was monitored daily with a Neubauer-improved counting chamber (Marienfield-Superior) and a light microscope (Olympus BX41) with a 40x magnification. The specific growth rate was determined as described in Fernandes, et al. 19 .

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Section: Solvent Extractionmentioning

confidence: 99%

Exploring Pavlova pinguis chemical diversity: a potentially novel source of high value compounds

Fernandes

Martel

Cordeiro

2020

Sci Rep

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“…Extraction of the lipophilic fraction. The lipophilic phase was extracted as reported in Ma et al (2015), with some modifications. Approximately 100-150 mg of freeze-dried sample was extracted with an aqueous solution (methanol:water, 1:1) and chloroform in a 1:1 ratio.…”

Section: Methodsmentioning

confidence: 99%

Hemiselmis andersenii and Chlorella stigmatophora As New Sources of High‐value Compounds: A Lipidomic Approach

Fernandes

Cordeiro

2020

Journal of Phycology

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To unlock the potential of Chlorella stigmatophora (Trebouxiophyceae, Chlorophyta) and Hemiselmis andersenii (Cryptophyceae, Cryptophyta) as natural reactors for biotechnological exploitation, their lipophilic extracts were characterized using Fourier Transform Infrared spectroscopy with Attenuated Total Reflectance (FTIR-ATR) and Gas Chromatography-Mass Spectrometry (GC-MS) before and after alkaline hydrolysis. The GC-MS analysis enabled the identification of 62 metabolites-namely fatty acids (27), aliphatic alcohols (17), monoglycerides (7), sterols (4), and other compounds (7). After alkaline hydrolysis, monounsaturated fatty acids increased by as much as 87%, suggesting that the esterified compounds were mainly neutral lipids. Hemiselmis andersenii yielded the highest Σω3/Σω6 ratio (7.26), indicating that it is a good source of ω3 fatty acids, in comparison to C. stigmatophora (Σω3/Σω6 = 1.24). Both microalgae presented significant amounts of aliphatic alcohols (6.81-10.95 mg Á g dw −1), which are recognized by their cholesterol-lowering properties. The multivariate analysis allowed visualization of the chemical divergence among H. andersenii lipophilic extracts before and after alkaline hydrolysis, as well as speciesspecific differences. Chlorella stigmatophora showed to be a valuable source of essential fatty acids for nutraceuticals, whereas H. andersenii, due to its high chemical diversity, seems to be suitable for different fields of application.

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“…Various drying techniques showed high efficiency for seaweeds, including oven drying [133], sun drying [134], and freeze drying [135]. In a previous study evaluating the impact of different drying methods on the composition of L. digitata, it was observed that the quantities of laminarin, the primary carbohydrate in brown seaweeds, were 10.9, 10.8, and 14.7% dw for oven-dried, frozen-oven dried, and freeze-dried seaweed, respectively [130,136]. These findings indicate that freeze-drying is a more viable option.…”

Section: Marine Biomass Conversion Into Bioethanolmentioning

confidence: 99%

Exploring the Prospects of Fermenting/Co-Fermenting Marine Biomass for Enhanced Bioethanol Production

Osman,

Abo-Shady,

Elshobary

et al. 2023

Fermentation

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With the rising demands for renewable fuels, there is growing interest in utilizing abundant and sustainable non-edible biomass as a feedstock for bioethanol production. Macroalgal biomass contains a high content of carbohydrates in the form of special polysaccharides like alginate, agar, and carrageenan that can be converted to fermentable sugars. In addition, using seagrass as a feedstock for bioethanol production can provide a sustainable and renewable energy source while addressing environmental concerns. It is a resource-rich plant that offers several advantages for bioethanol production, including its high cellulose content, rapid growth rates, and abundance in coastal regions. To reduce sugar content and support efficient microbial fermentation, co-fermentation of macroalgae with seagrass (marine biomass) can provide complementary sugars and nutrients to improve process yields and economics. This review comprehensively covers the current status and future potential of fermenting macroalgal biomass and seagrass, as well as possible combinations for maximizing bioethanol production from non-edible energy crops. An overview is provided on the biochemical composition of macroalgae and seagrass, pretreatment methods, hydrolysis, and fermentation processes. Key technical challenges and strategies to achieve balanced co-substrate fermentation are discussed. The feasibility of consolidated bioprocessing to directly convert mixed feedstocks to ethanol is also evaluated. Based on current research, macroalgae-seagrass co-fermentation shows good potential to improve the bioethanol yields, lower the cost, and enable more optimal utilization of diverse marine biomass resources compared to individual substrates.

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Optimization of cell disruption methods for efficient recovery of bioactive metabolites via NMR of three freshwater microalgae (chlorophyta)

Cited by 11 publications

References 25 publications

Exploring Pavlova pinguis chemical diversity: a potentially novel source of high value compounds

Exploring Pavlova pinguis chemical diversity: a potentially novel source of high value compounds

Hemiselmis andersenii and Chlorella stigmatophora As New Sources of High‐value Compounds: A Lipidomic Approach

Exploring the Prospects of Fermenting/Co-Fermenting Marine Biomass for Enhanced Bioethanol Production

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