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In the Haptophyta Pavlova pinguis J. C. Green 16 only specific classes of compounds have been analyzed to assess its potential as food for larval hatcheries in aquaculture and as ecological biomarkers 13,17,18. For instance, Milke, et al. 11 and Parrish, et al. 12 assessed the ability of P. pinguis and other Pavlova species to sustain postlarval sea scallop growth focusing on its proximate, fatty acid and sterol composition. In this microalga the complete characterization of lipid components (simple and complex lipids) is still largely unexplored 6,9. Thus, in the present study the analysis of the lipophilic fraction of P. pinguis was performed in order to identify its lipophilic features before and after alkaline hydrolysis through gas chromatography-mass spectrometry (GC-MS) and evaluate its prospects for further improvement in bioactive compounds. Materials and Methods Growth and culture conditions. The haptophyta Pavlova pinguis (RCC 1539) was obtained from the Roscoff Culture Collection (RCC). The microalgal cultures were made by inoculating starter cultures into 1L of sterile f/2-Si medium with pH adjusted to 7.0 under 70 μmol m −2 s −1 light intensity with 16:8 h (light: dark cycles) at 25 °C. At the end of the logarithmic phase, the medium was centrifuged for 7 min. at 3720 g and the pellets washed. Microalgae growth was monitored daily with a Neubauer-improved counting chamber (Marienfield-Superior) and a light microscope (Olympus BX41) with a 40x magnification. The specific growth rate was determined as described in Fernandes, et al. 19 .

We present a case of brain abscess caused by Nocardia otitidiscaviarum in an immunocompromized 44-y-old male. Only 7 other cases of N. otitidiscaviarum brain abscess or involvement were found in the literature. The mortality was 75% despite treatment among cases reviewed. There is a lack of therapeutic guidelines regarding brain abscesses due to Nocardia.

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