Optimization of Blastocyst Vitrification May Require Artificial Collapse

Fischer, Erin; Givens, C.; Conaghan, J.

doi:10.1016/j.fertnstert.2011.01.047

Cited by 1 publication

(2 citation statements)

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“…Several papers whose authors experimented the application of the laser collapse technique in expanded blastocysts demonstrated its efficiency, with improvement in survival rate, pregnancy rate and implantation rate, reducing cancellations of transfers after thawing. 12,20,31 On the other hand, in the research conducted by Marcos et al, 32 the authors affirm that the collapse reduced the implantation potential of the transferred blastocysts. They reported that the collapse could act adversely in the implantation, either because it affects the quality of the embryo or by directly reducing its implantation capacity resulting in lower rates of clinical pregnancy.…”

Section: Discussionmentioning

confidence: 93%

“…4 In order to overcome this problem many researchers presented methods for obtaining collapse of the blastocele prior to vitrification, with purpose of increasing the success of the technique. 12,[18][19][20][21] The collapse of blastocysts can be induced by mechanical means such as repeated pipetting with micropipettes of different diameters or by drilling the blastocyst using micro needles of different calibers. 19 Recently, the directed laser pulse was proposed as an alternative to mechanical collapse, and its use is currently widespread.…”

Section: /7mentioning

confidence: 99%

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Laser collapse performance in blastocysts applied before vitrification

Macedo¹,

Oliveira²,

Gomes³

et al. 2018

Hum. Reprod. Arch.

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The objetive of the present study was to investigate the effect of the reduction of blastocele fluid before vitrification with the application of collapsing and the effects of this procedure on the expected results of in vitro fertilization protocols. Methods: A retrospective casecontrol study was conducted with patients who sought assisted fertilization services from January 2015 to October 2017 at Reproferty Clinic. After submission to the ethics committee, data from 106 patients were divided into two groups: 42 cases represented by those who had artificially collapsed blastocysts per laser pulse before vitrification and 64 controls corresponding to patients where artificial shrinkage of expanded blastocysts was not performed. By statistical analysis, the means and standard deviation of each group were obtained, which were compared by the Mann-Whitney test, at a 95% significance level. Results: the results showed that there was statistical significance comparing the group of cases and controls in the parameters related to embryo freezing, survival and transference as well as to the rates of positive beta human chorionic gonadotropin, clinical pregnancy and live births. Conclusions: the study demonstrated evidence that vitrification after artificial laser collapse of the blastocyst is safe, effective, and more efficient than vitrification of non-collapsed blastocysts in vitro fertilization protocols, however further studies are needed to evaluate the efficiency of artificial shrinkage in routine protocols.

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