Optimization of biosensing using grating couplers: immobilization on tantalum oxide waveguides

Real-Time Observation of Affinity Reactions Using Grating Couplers: Determination of the Detection Limit and Calculation of Kinetic Rate Constants

“…Since this cube has two binding sites on two tran matrix, which was coupled covalently on the senopposite sides, one ends up with the factor 1/3. We sor surface (20). This approach provided more accurate agree that there is some freedom in choosing the binding assays.…”

“…Excess reagent was rinsed off and the dextran-coated Kinetic rate constants were determined for two af-sensors were incubated in 1 M bromoacetic acid in 2 finity systems: the avidin-biotin interaction and the M NaOH for 16 h resulting in carboxymethyldextranprotein G-human immunoglobulin (HIgG) binding. coated waveguides (20). Carboxymethyldextran-coated The association rate constants of the different biotinyl-sensors were activated using a solution of 0.1 M 1-ethylated proteins were calculated from single binding 3-(3-dimethylaminopropyl)carbodiimide hydrochloride curves on condition that the dissociation of the complex and 0.1 M N-hydroxysulfosuccinimide in distilled water could be neglected.…”

Nanocomposites: From Fabrications to Electrochemical Bioapplications

“…It is known that Protein A is able to selectively bind the F c regions of various mammalian immunoglobulins while leaving the F ab regions available for antigen binding [183]. One of important findings in this work is that the spacer not only improves the protein attachment but also facilitates the interaction between Protein A and the F c region of IgG.…”

Laser-written nanoporous silicon diffraction gratings for biosensors

“…These variations can be detected by measuring light diffraction intensities. Tantalum pentoxide diffraction grating sensors were reported to study dynamic response of antibody interaction with protein G adsorbed on the sensing surface [4]. Hydrogel grating was used to detect the glucose concentration [5].…”