Real-Time Observation of Affinity Reactions Using Grating Couplers: Determination of the Detection Limit and Calculation of Kinetic Rate Constants

A Particle-Level Model of Irreversible Protein Adsorption with a Postadsorption Transition

“…Keeping in mind that ϭ K s , we may now integrate Eq. [3] forward in time to obtain the total adsorbed density.…”

“…In Eq. [3], the partial derivative is taken at constant K s . ⌽ (°) is approximated by one of its very accurate interpolants,…”

“…The adsorption of proteins at a liquid-solid interface provides the basis for certain chromatographic separation techniques (1) and is used to form layers that can act as biosensors (2,3). The thrombotic response to an in vivo foreign body is initiated by the adsorption of plasma proteins (4) and can also be passivated by preadsorption of nonthrombogenic proteins such as albumin (5).…”

Simply Modified Biosensor for the Detection of Human IgG Based on Protein AModified Porous Silicon Interferometer

“…[1][2][3][4][5] The PSi optical sensing features were based on the changes of its photonic properties, such as photoluminescence or reflectance, on exposure to the gaseous or liquid substances. [6][7] Two main optical transduction methods for label-free biosensors were both optical interferometric methods based on interferometers, 8 evanescent wave devices, 9 and grating couplers, 10 and surface plasmon methods based on metal films, 11 and colloids. 12 However they required a tedious work and very complex preparation during analysis.…”

Peer Reviewed: Can Affinity Sensors Be Used To Detect Food Contaminants?

“…Thus, the lowest detection limit for a compound depends on the sensitivity of the device to monitor small changes in refractive index and on the compound's molecular weight. Changes in refractive index are directly proportional to the mass loading due to the binding of the analyte to the waveguide surface (18,20).…”