Optimization of biodegradable PEG/PLGA nanofiber mats electrospinning process for anti‐adhesion application

Abstract

Electrospinning is a direct, continuous, and useful technique to prepare nanofiber by applying electrostatic forces. In this study, poly(lactic‐co‐glycolic acid)/poly(ethylene glycol) (PLGA/PEG) nanofiber mats were prepared, and electrospinning process was optimized to obtain appropriate fiber diameter and hydrophilicity for anti‐adhesion application. Optimization of applied voltage, PEG content, and feeding rate was investigated using response surface methodology. A total of 15 trials were designed to optimize the parameters. Fiber diameter was measured using scanning electron microscopy. Individual and interactive effects of the solution properties were determined. Moreover, the adequacy of the models was verified by validation experiments. For anti‐adhesion test, a nanofiber mat was produced based on the suggested optimum electrospinning conditions. Results showed that optimum fiber diameters were obtained using 7.5% PEG content, applied voltage of 19 kV, and flow rate of 3 mL/h. Experimental results were in good agreement with the predicted fiber diameters. Furthermore, a rat model of sidewall defect‐cecum abrasion was employed to investigate the efficacy of PEG/PLGA in preventing postoperative peritoneal adhesions. Hence, this study provides an overview on the fabrication of PLGA/PEG nanofibers with targeted diameter, which may be used in anti‐adhesion. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46282.

Enhanced Diabetic Wound Healing Using Electrospun Biocompatible PLGA-Based Saxagliptin Fibrous Membranes

“…Additionally, the pristine electrospun fibers showed higher elongation at breakage (218.6 ± 24.2%) than the saxagliptin/PLGA electrospun fibers (134.6 ± 6.7%) ( p = 0.004). The concentration of PLGA, applied voltage, and flow rate are parameters that critically affect the size of the electrospun fiber diameter and the uniformity [ 24 ]. With a fixed high voltage (35 kV) and flow rate (3.6 mL per hour) [ 21 ], the decrease in the PLGA concentration in the solution yielded fibers of smaller diameter, mainly owing to the reductions in the polymer viscosity and the degree of chain entanglements [ 25 , 26 , 27 ].…”

Fabrication, characterization and evaluation of the effect of PLGA and PLGA–PEG biomaterials on the proliferation and neurogenesis potential of human neural SH‐SY5Y cells

“…The water contact angle index of nanofibrous scaffolds reflects surface hydrophilicity which is integral to biocompatibility properties in in vivo conditions. It was previously demonstrated that the cell attachment, flattening and proliferation can be correlated with contact angle value (Li et al, 2016; Wang et al, 2018; Zhao et al, 2018). In the current experiment, the contact angle of the pure PLGA fiber membrane reached 62.60° (Figure 1d).…”

“…The contact angle is commonly used to assess biomaterials wettability (Zhao et al, 2018). The water contact angle index of nanofibrous scaffolds reflects surface hydrophilicity which is integral to biocompatibility properties in in vivo conditions.…”

Study on the preparation and activity of intelligent response poly(lactic-co-glycolic acid)-ss-polyethylene glycol copolymer micelles

“…A strong peak at 1757 cm -1 might be the stretching vibration of C=O. 12 As shown in Figure 3(b), the characteristic absorption peaks of PEG at 3313, 2885, 1375, 1113, 949, and 844 cm -1 represented ν(OH), νs (CH 2 ), νs(CH 3 ), ν(C-O), δ(C-O) and δ(CH 2 CH 2 O), respectively, 25 and these characteristic peaks were also observed in the synthesized copolymer PLGA-ss-PEG. In addition, some blue shifts accrued in FT-IR spectra after a chemical reaction, and -N-H stretching vibration at 3500-3300 cm -1 , -N-H bending vibration and -C=O stretching vibration at 1646.0 cm -1 and 1561.3 cm -1 , respectively, v(C-N) stretching vibration at 1360-1020 cm -1 .…”