Optimization of bacteriocin production by Lactobacillus sp. MSU3IR against shrimp bacterial pathogens

Abstract

BackgroundAquaculture is one amongst the growing and major food producing sectors. Shrimp culture is one of the subsectors of aquaculture that attracts more attention because of the economic interest. However, the shrimp culture systems have been facing severe consequences and economical losses due to disease outbreaks. Risk of disease outbreak can be combated with the application of probiotics. For economically viable production of such probiotic products, the present study provides information on the optimization and partial purification of bacteriocin produced by a goat milk isolate Lactobacillus sp. MSU3IR against the shrimp bacterial pathogens.ResultsBacteriocin production was estimated as a measure of bactericidal activity (arbitrary Unit/ml) over the test strains. The optimum culture conditions and media components for maximum bacteriocin production by Lactobacillus sp. MSU3IR were: pH: 5.0, temperature: 30°C, carbon source: lactose; nitrogen source: ammonium acetate; NaCl: 3.0% and surfactant: Tween 80. MRS medium was found to extend better bacteriocin production than other tested media. Upon partial purification of bacteriocin, the SDS-PAGE analysis had manifested the presence of two peptide bands with the molecular weight of 39.26 and 6.38 kDa, respectively.ConclusionThe present results provide baseline trend for the statistical optimization, scale up process and efficient production of bacteriocin by the candidate bacterial strain Lactobacillus sp. MSU3IR which could be used to replace the usage of conventional chemotherapeutics in shrimp culture systems.

Biological potency and characterization of antibacterial substances produced by Lactobacillus pentosus isolated from Hentak, a fermented fish product of North-East India

“…Our study strongly favours the findings of Iyapparaj et al (2013), who demonstrated the maximum production of bacteriocin from lactic acid bacteria at pH 5.0. On the other hand, our results showed partial agreement with the findings of Zamfir et al (2000), Aasen et al (2000), Yang and Ray (1994) and Todorov and Dicks (2005), who observed maximum bacteriocin production in the range of pH 4.5-6.0.…”

“…Growth temperature and antagonistic substance production from lactic acid bacteria are often correlated, as indicated in the present report. Our study favours the findings of Iyapparaj et al (2013) and Moonchai et al (2005), who reported that the production of antibacterial substances from LAB was maximal at 30 °C. The present investigation was in complete agreement with the finding of Ekhay et al (2013), who demonstrated that maximal antibacterial substance production by the bacterium correlates with the optimal cell growth temperature.…”

Exploring Anti-Klebsiella Pneumoniae Activity of Probiotic Lactobacilli of Curd Origin

“…as 'a measure of production of bioactive components' using the formula mentioned elsewhere [14]: AU/mL = ; where ZDI denotes "zone diameter of inhibition", following agarwell diffusion method.…”

Effect of Heat Stress on Yield, Monoterpene Content and Antibacterial Activity of Essential Oils of <em>Mentha </em>X <em>Piperita</em> Var. Mitcham and <em>Mentha Arvensis</em> Var. <em>Piperascens</em>

“…Briefly, 5 μL of essential oil were placed on Muller Hinton agar plates which were overlaid with approximately 8 mL of soft agar (0.7%) pre-mixed with 10 μL of Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, and Bacillus cereus strains grown for 24 h at 37 °C. Plates were incubated overnight at 37 °C and the anti-microbial activity was calculated as a measure of anti-microbial molecule production, according to Equation 1 [22,23]:…”